Analysis of combined screening results for hearing and deafness in 842 newborns in Dalian / 中国医师进修杂志

Objective:To analyze the results of the joint screening of newborn hearing and deafness genes in Dalian to provide a reference for the prevention and control of hereditary deafness.Methods:Eight hundred and forty-two neonates born in Dalian Women and Children′s Medical Group from January 1, 2022 to May 30, 2022 were screened retrospectively, using AABR (automatic brainstem evoked potential). And 20 mutation sites of common genetic deafness 4 genes , including GJB2, GJB3, SLC26A4 (PDS) and mitochondrial genes associated with drug-induced deafness (MT-RNRI)(12SrRNA), were detected by high-throughput sequencing.Results:Among the 842 newborns, 840 passed hearing screening (99.8%); 36 cases (4.3%) passed the hearing screening but not the hearing loss gene screening; 804 cases passed through the both screening (95.5%); 2 cases (0.24%) failed in the both screening. 38 cases of deafness gene mutations were detected, with a total carrying rate of 4.51% (38/842). Among them, the carrying rates of heterozygous mutations in GJB2, GJB3, SLC26A4 (PDS), MT-RNRI (12SrRNA) were 1.90%, 0.24%, 1.30%, and 0.95%, respectively. The carrying rates of GJB2/GJB3 composite heterozygous mutations were 0.12%.Conclusions:The combined screening of neonatal hearing and deafness genes can reduce the missed rate of hearing screening. The carrier rate of neonatal deafness gene in Dalian is 4.51%, with the highest GJB 2 carrier rate, followed by SLC26A4 (PDS) carrier rate.

Teoría de las representaciones sociales: Una revisión de la literatura / Teoria das representações sociais: Uma revisão deliteratura / Theory of social representations: A review of the literature

Las Representaciones Sociales son un conjunto de conocimientos, opiniones e imágenes que nos permiten evocar un evento, persona u objeto en particular. Estas representaciones son el resultado de la interacción social, que son comunes a un determinado grupo de individuos. El representante principal de la Teoría de las Representaciones Sociales es Serge Moscovici. Este artículo tiene como objetivo discutir la Representación Social a partir de una revisión de la literatura sobre la a) Historia de la Psicología Social y de las Representaciones Sociales; b) Teoría de las Representaciones Sociales como una forma de conocerse a sí mismo; c) Representación social, historia y memoria colectiva del sujeto; d) Objetivación y anclaje: la dinámica sociocognitiva de las representaciones sociales; e) La Teoría de las Representaciones Sociales como metodología de investigación; y f) Teoría de las Representaciones Sociales y la superación de algunas dicotomías AU

As Representações Sociais são oconjunto de conhecimentos, opiniões e imagens que nos permitem evocar um determinado acontecimento, pessoa ou objeto. Essas representações são o resultado da interação social, que são comuns aum determinado grupo de indivíduos. O principal representante da Teoria das Representações Sociais é Serge Moscovici. Este artigo tem como objetivo discorrer sobre a Representacao Social a partir de uma revisão da literatura sobre a) História da Psicologia Social e Representações Sociais; b) Teoria das Representações Sociais como forma de se conhecer; c) Representação social, história e memória coletiva do sujeito; d) Objetivação e ancoragem: a dinâmica sociocognitiva das representações sociais; e) A Teoria das Representações Sociais como metodologia de pesquisa; e f) Teoria das Representações Sociais e superação de algumas dicotomias AU

Social Representations are the set of knowledge, opinions and images that allow us to evoke a particular event, person or object. These representations are the result of social interaction, which are common to a certain group of individuals. The main representative of the Theory of Social Representations is Serge Moscovici. This article aims to discuss Social Representation from a literature reviewon a) History of Social Psychology and Social Representations; b) Theory of Social Representations as a way of getting to know each other; c) Social representation, history and collective memory of the subject; d) Objectification and anchoring: the socio-cognitive dynamics of social representations; e) The Theory of Social Representations as a research methodology; and f) Theory of Social Representations and overcoming some dichotomies AU

Experimental study of magnetic anchoring and traction device assisting thoracoscopic esophagectomy / 中国胸心血管外科临床杂志

@#Objective    To verify the feasibility of a self-designed magnetic anchoring and traction device (MATD) for assisting two-port video-assisted thoracoscopic esophagectomy. Methods    Three Beagle dogs were selected as animal models with age ranging from 1-6 years and weight ranging from 8-12 kg, and they underwent two-port video-assisted thoracoscopic esophagectomy after general anesthesia. We used the MATD to retract the esophagus to different directions, which assisted mobilizing esophagus, detecting the nerves along esophagus and dissecting paraesophagus lymph nodes. The operation time, blood loss and feasibility of the MATD were recorded. Results    With the aid of the MATD, we successfully retracted and mobilized the esophagus, detected the nerves and dissected the lymph nodes in three Beagle dog models. During the operation, the MATD provided sufficient and steady traction of esophagus to achieve a good exposure of the operative field, effectively decreasing the interference between working instruments. The MATD worked well. The mean operation time was 30 min, and the mean intraoperative blood loss was about 10 mL. Conclusion    It is effective to use the MATD to assist retracting esophagus during video-assisted thoracoscopic esophagectomy. The magnetic anchoring and traction technique can assist to expose the surgical field, decrease the interference between the working instruments and have the potential clinical application.

Una Descripción General y Actualizada de Miniplacas y Minitornillos. Efectos Dentoalveolares y Esqueléticos / A General and Updated Description of Miniplates and Miniscrews. Dentoalveolar and Skeletal Effects

RESUMEN: En ortodoncia, las miniplacas se utilizan como dispositivo de anclaje temporal (TAD) para la realización de movimientos dentales que permiten el uso de fuerzas ortopédicas en ellos. En comparación con los mini tornillos, las miniplacas tienen la ventaja de una tasa de falla muy baja, pero la desventaja es que para la extracción se necesita el mismo acto quirúrgico que se realizó para la instalación. El objetivo de este estudio es realizar una revisión bibliográfica de las indicaciones de miniplacas en pacientes con mordidas abiertas, clase II y anomalías de clase III, y buscar cómo las miniplacas han mejorado los tratamientos de ortodoncia. La información principal se reunió buscando en PubMed con las palabras clave enumeradas a continuación. Afirmamos que las miniplacas están indicadas para la retracción en masa de la arcada, donde se observó que la fuerza de 150 g aplicada en los molares superiores es suficiente no solo para empujar los molares hacia atrás en una clase I corregida, sino también para iniciar la retracción de premolares, caninos e incisivos. En pacientes con mordida abierta, las miniplacas se definen como un método seguro, una alternativa rápida y menos costosa a la cirugía ortognática. Y en pacientes de las clases II y III se utilizan sin producir efectos dentoalveolares que sustituyan a los dispositivos extraorales como máscaras, con dispositivos intraorales y elásticos (BAMP).

ABSTRACT: In orthodontics, miniplates are used as a Temporary Anchoring Device (TAD) for the purpose dental movements, allowing the use of orthopedic forces. In comparison with mini-screws, miniplates have the advantage of a very low rate of failure. Nonetheless, their removal requires the same surgical procedure as during installation, which is an obvious disadvantage. The aim of this study is to review the indications of miniplates in patients with open bite, class II and class III anomalies, and review how miniplates improved orthodontics treatments. Information was obtained by a search in PubMed with the keywords listed below. Miniplates are indicated for retraction in mass of the arcade, where it was seen that the force of 150 g applied on maxillary molars, is sufficient not only to push the molars back into a corrected class I, but also to initiate retraction of premolars, canines, and incisors. In open-bite patients, mini plates, are achieved as a safe method, that is quick and a less expensive alternative to orthognathic surgery. Further, in class II and III patients they are used without producing dentoalveolar effects replacing extraoral devices as facemasks, with intraoral devices and elastics. (BAMP).

Application of magnetic surgery technique in thoracic surgery / 中国胸心血管外科临床杂志

@#The earliest research of magnetic surgery was the application of magnetic anastomotic device to anastomose the blood vessels. Now, it has been widely used for anastomosis of blood vessels, gastrointestinal tract and biliary tract. The concept of "magnetic surgery" was named firstly by LU Yi in 2010 and magnetic surgery was classified into magnetic anchoring technique, magnetic navigation technique, magnetic compression technique, magnetic tracing technique, and magnetic suspension technique. The applications of magnetic surgery in the field of thoracic surgery mainly include magnetic compression technique, magnetic anchoring technique and magnetic navigation technique. This paper summarizes the application of magnetic surgery in thoracic surgery and prospects its future development in the field of thoracic surgery.

Memória coletiva e teoria das representações sociais: confluências teórico-conceituais / Collective memory and social representations theory: a theoretical-conceptual confluence

Este artigo objetiva apresentar os principais aspectos teóricos e conceituais de duas teorias: Memória Coletiva, termo cunhado por Maurice Halbwachs (1877-1945) e a Teoria das Representações Sociais, pensada pelo psicólogo Serge Moscovici (1928-2014), bem como de que maneira esses dois campos se confluem, tendo em vista a influência sociológica de Émile Durkheim (1858-1917). Esse estudo reúne elementos teórico-conceituais do pensamento durkheimiano, que pode ampliar a discussão acerca das articulações entre as duas perspectivas teóricas apresentadas. Sob esse enfoque epistemológico, a discussão entre Memória Coletiva e Teoria das Representações Sociais tem sido pouco enfatizada nas produções científicas que se propuseram a concatenar as duas teorias, o que imprime originalidade ao presente trabalho. É perceptível uma relação de similaridade entre o processo de ancoragem moscoviciano com os quadros sociais da memória, sendo que ambos dependem de processos relacionais para construção social das práticas cotidianas e da capacidade de teorização da realidade social.

This paper aims to present the main theoretical and conceptual aspects of two theories: Collective Memory, a term coined by Maurice Halbwachs (1877-1945) and Social Representations Theory, developed by the psychologist Serge Moscovici (1928-2014), and how both fields converge, considering the sociological influence of Emile Durkheim (1858-1917). This study brings together theoretical and conceptual elements of Durkheim´s thought, which can broaden the discussion about the connections between the theoretical perspectives presented. Under this epistemological approach, the discussion between Collective Memory and Social Representations Theory has been little emphasized in the scientific productions that have proposed to concatenate the two theories, which brings originality to the present work. It is noticeable the similarity between the anchoring process of Moscovici and the social frames of memory, both of which depend on relational processes for the social construction of daily practices and the ability to theorize social reality.

The T-loop in details

ABSTRACT Introduction: The T-loop as designed by Burstone is a space closure spring used in the rational application of orthodontic biomechanics. Despite the diversity of studies, there is still no consensus on the optimal parametric characteristics for its conformation. Objective: This study aimed at reviewing the literature on the force systems released by different conformations of the T-loop, according to the type of anchorage and the main characteristics and factors that influence them. Results: Comparing the studies, the need for standardization was perceived in the methodology to shape the loops, regarding the variables that influence the force system. Most of the experimental studies with this loop do not report the vertical movement, nor the steps and angles that occur in the brackets. Clinical studies have obtained more variable results in relation to vertical acting forces, considering the influence of chewing. Conclusion: There is great potential for future studies with this type of loop, especially using nickel-titanium alloys, in order to achieve a pure translational movement without friction, with optimal and constant levels of force.

RESUMO Introdução: a alça T preconizada por Burstone é um instrumento de fechamento de espaços utilizado na aplicação racional da biomecânica ortodôntica, dentro da técnica do arco segmentado. Apesar da diversidade de estudos, ainda não há consenso quanto às características paramétricas ótimas para a sua conformação. Objetivo: esse estudo teve como objetivo revisar a literatura sobre os sistemas de forças liberados por diferentes conformações da alça T, de acordo com o tipo de ancoragem necessária, salientando suas principais características e os fatores que as influenciam. Resultados: comparando-se os estudos, foi perceptível a necessidade de padronização da metodologia usada para conformar as alças, levando em consideração as variáveis que exercem influência no sistema de forças, de forma a se obter sistemas mais acurados, permitindo a realização de testes mais precisos e uma comparação mais eficaz dos estudos. A maioria dos estudos experimentais com esse tipo de alça não relata a movimentação vertical, nem os degraus e ângulos que ocorrem nos braquetes. Estudos clínicos obtiveram resultados menos rígidos em relação às forças verticais atuantes, levando em consideração a influência da mastigação. Conclusões: há grande potencial para futuros estudos com esse tipo de alça, especialmente utilizando ligas de níquel-titânio, para que se chegue cada vez mais perto de um movimento de translação puro e sem atrito, com níveis de força ótimos e constantes.

Roles of AKAP1 in high-glucose induced mitochondrial fission in podocytes / 中华肾脏病杂志

Objective To investigate the roles of A kinase anchoring protein1(AKAP1)in high-glucose induced mitochondrial fission in podocytes.Methods Conditionally immortalized human podocytes were cultured in serum-free medium for 24 hours,and then exposed to different glucose concentration conditions in different time periods.The protein expressions of AKAP1 were observed by immunofluorescence,and AKAP1,dynamin related protein1 (Drp1) and phospho Ser 637-Drp1 (p-Drp1)were analyzed by Western blotting.AKAP1 siRNA was transfected to block AKAP1 expression.Podocytes were then divided into normal control group (5 mmol/L glucose),hypertonic group (30 mmol/L mannitol+5 mmol/L glucose),high glucose group (35 mmol/L glucose),and high glucose+AKAP1 siRNA group.Mitochondrial morphological changes were assessed by mitotracker red staining.Podocyte apoptosis was assessed by flow cytometry.Results Compared with normal group,high-glucose induced more podocytes apoptosis (P ＜ 0.05),more mitochondrial fission with decreased aspect ratio and form factor (all P ＜ 0.05).Upregulated AKAP1 protein level,and increased ratio of p-Drp1/Drp1 (all P ＜ 0.05) in time and concentration dependent manners were also obscrvcd.Compared with high glucose group,transfection of AKAP1 siRNA showed less apoptosis (P ＜ 0.05),less mitochondrial fission with increased aspect ratio and form factor (all P ＜ 0.05),and down-regulated AKAP1 protein level as well as p-Drp1/Drp1 ratio (all P ＜ 0.05).Conclusion High glucose induced mitochondrial fission might be induced through AKAP1-Drp1 pathway.

Representações Sociais de Mulher Cigana entre População Não-Cigana Brasileira e Italiana: Ancoragem Psicológica e Social / Social Representations of the Gypsy Woman among Non-Gypsy Brazilian and Italian Population: Psychological and Social Anchoring

RESUMO Referenciados pela abordagem não consensual da Teoria das Representações Sociais, objetivou-se investigar os processos de ancoragem psicológica e social frente às representações sociais de mulher cigana, o que permite analisar a modulação dos objetos sociais a partir do posicionamento interindividual e das experiências compartilhadas pelos sujeitos da representação segundo seu contexto sociocultural de inserção. Participaram do estudo 643 sujeitos não ciganos, brasileiros e italianos, com idade média de 22,81 anos (DP=5,73). Aplicando a análise de correspondência lexical, o processo de ancoragem psicológica indicou a formulação de oito clusters, que caracterizam diferentes representações, segundo as dimensões: mágico-religiosa, maternidade e cuidado com o grupo-família, exclusão social e figuras do feminino. Por meio da análise do processo de ancoragem social, verificou-se que variáveis como contato, sexo e nacionalidade atuam na modulação dessas diferentes representações sobre o objeto, produzindo ambiguidades que, historicamente, têm orientado processos de discriminação contra grupos ciganos.

ABSTRACT Based on the non-consensual approach of the Theory of Social Representations, the present study aimed to investigate the processes of social and psychological anchoring regarding the social representations of the gypsy woman, dimension which allows analyzing the modulation of the social objects from the interindividual positioning and the experiences shared by subjects of the representation according to their sociocultural context of insertion. The study included 643 non-gypsy, Brazilian and Italian individuals with mean age of 22.81 years (SD=5.73). Once lexical correspondence analysis was conducted, the process of psychological anchoring indicated the formation of eight clusters, which characterize different representations, according to the following dimensions: magical-religious, maternity and care of the family-group, social exclusion, and female figures. Through the analysis of the social anchoring process, we verified that variables such as contact, gender, and nationality act in the modulation of these different representations over the object, producing ambiguities that have historically led to processes of discrimination against gypsy groups.

How to unleash mitochondrial apoptotic blockades to kill cancers?

Apoptosis, especially the intrinsic mitochondrial cell death pathway, is regulated by the BCL-2 family of proteins. Defects in apoptotic machinery are one of the main mechanisms that cells employ to evade cell death and become cancerous. Targeting the apoptotic defects, either by direct inhibition of BCL-2 family proteins or through modulation of regulatory pathways, can restore cell sensitivity to cell death. This review will focus on the aspects of BCL-2 family proteins, their interactions with kinase pathways, and how novel targeted agents can help overcome the apoptotic blockades. Furthermore, functional assays, such as BH3 profiling, may help in predicting responses to chemotherapies and aid in the selection of combination therapies by determining the mitochondrial threshold for initiating cell death.

Animal model experiment on application of new magnetic anchoring and guidance system on endoscopic submucosal dissection(with video) / 中华消化内镜杂志

Objective To evaluate the safety and efficacy of a new magnetic anchoring and guidance system on endoscopic submucosal dissection(ESD)in pig models. Methods Two skilled operators performed ESD at the rectum part of 2 pigs with the help of a new magnetic anchoring and guidance system. The dissection time,dissection rate per unit time, en-bloc resection rate and complications were analyzed. Results Five simulated lesions underwent ESD with the help of the magnetic anchoring and guidance system. The dissection time was 26.6 min and the dissection rate was 0.38± 0.1 cm2/min. All the lesions were dissected totally. There was no perforation or delayed bleeding. Conclusion The new magnetic anchoring and guidance system is effective and safe for pig rectal ESD. It can effectively expose submucosal layer,and assist resection under the endoscopic observation.

Cluster hepaticojejunostomy with radial spreading anchoring traction technique for secure reconstruction of widely opened hilar bile ducts

Secure reconstruction of multiple hepatic ducts that are severely damaged by tumor invasion or iatrogenic injury is a challenge. Failure of percutaneous or endoscopic biliary stenting requires lifelong placement of one or more percutaneous transhepatic biliary drainage (PTBD) tubes. For such difficult situations, we devised a surgical technique termed cluster hepaticojejunostomy (HJ), which can be coupled with palliative bile duct resection. The cluster HJ technique consisted of applying multiple internal biliary stents and a single wide porto-enterostomy to the surrounding connective tissues. The technique is described in detail in the present case report. Performing cluster HJ benefits from three technical tips as follows: making the multiple bile duct openings wide and parallel after sequential side-to-side unification; radially anchoring and traction of the suture materials at the anterior anastomotic suture line; and making multiple segmented continuous sutures at the posterior anastomotic suture line. Thus, cluster HJ with radial spreading anchoring traction technique is a useful surgical method for secure reconstruction of severely damaged hilar bile ducts.

A comparison of strength for two continuous peripheral nerve block catheter dressings / 대한마취과학회지

BACKGROUND: Despite the benefits of continuous peripheral nerve blocks, catheter dislodgment remains a major problem, especially in the ambulatory setting. However, catheter dressing techniques to prevent such dislodgment have not been studied rigorously. We designed this simulation study to test the strength of two commercially available catheter dressings. METHODS: Using a cadaver model, we randomly assigned 20 trials to one of two dressing techniques applied to the lateral thigh: 1) clear adhesive dressing alone, or 2) clear adhesive dressing with an anchoring device. Using a digital luggage scale attached to a loop secured by the dressing, the same investigator applied steadily increasing force with a downward trajectory towards the floor until the dressing was removed or otherwise disrupted. RESULTS: The weight, measured (median [10th–90th percentile]) at the time of dressing disruption or removal, was 1.5 kg (1.3–1.8 kg) with no anchoring device versus 4.9 kg (3.7–6.5 kg) when the dressing included an anchoring device (P < 0.001). CONCLUSIONS: Based on this simulation study, using an anchoring device may help prevent perineural catheter dislodgement and therefore premature disruption of continuous nerve block analgesia.

S-palmitoylation regulates AMPA receptors trafficking and function: a novel insight into synaptic regulation and therapeutics

Glutamate acting on AMPA-type ionotropic glutamate receptor (AMPAR) mediates the majority of fast excitatory synaptic transmission in the mammalian central nervous system. Dynamic regulation of AMPAR by post-translational modifications is one of the key elements that allow the nervous system to adapt to environment stimulations. S-palmitoylation, an important lipid modification by post-translational addition of a long-chain fatty acid to a cysteine residue, regulates AMPA receptor trafficking, which dynamically affects multiple fundamental brain functions, such as learning and memory. In vivo, S-palmitoylation is controlled by palmitoyl acyl transferases and palmitoyl thioesterases. In this review, we highlight advances in the mechanisms for dynamic AMPA receptors palmitoylation, and discuss how palmitoylation affects AMPA receptors function at synapses in recent years. Pharmacological regulation of S-palmitoylation may serve as a novel therapeutic strategy for neurobiological diseases.

Clinical outcome of artificial cervical disc arthroplasty combined with anchoring cervical intervertebral fusion cage for the treatment of multilevel cervical disc herniation / 中国医师进修杂志

Objective To evaluate the clinical outcome of artificial cervical disc arthroplasty combined with anchoring cervical intervertebral fusion cage (ACIFC) in the treatment of multilevel cervical disc herniation. Methods From October 2011 to September 2013, 0.26 patients with multilevel cervical disc herniation underwent artificial cervical disc arthroplasty combined with ACIFC, there were 10 male and 16 female with age from 32 to 60 years (mean 46.8 years). Two segment were involved in 15 patients, three segment were involved in 11 patients. Neck disability index (NDI) and Japanese Orthopaedic Association (JOA) score were used to evaluate neurofunctional recovery preoperatively and postoperatively. The postoperative stabilization and the range of motion (ROM) of implanted disc and the fusion of cage were observed on dynamic radiograph. Results The average operation time and amount of bleeding were (95±15) min and (100±20) ml respectively. The average postoperative follow-up was 15 months(range 6-20 months). At the end of follow up,the scores of JOA was increased and the scores of NDI was decreased:(9.08±1.72) scores vs. (14.88±1.37) scores, (40.31±4.97) scores vs. (25.23±4.10) scores,there had significant differences (P0.05). Solid fusion was achieved in all levels brought by cage.There was no cage subsidence or displacement. All artificial cervical disc achieved definite stabilization, no intraoperative and postoperative complication was noted. Conclusions Artificial cervical disc arthroplasty combined with ACIFC provides a feasible way for the treatment of multilevel cervical disc herniation. The definite stabilization and maintained ROM can reduce the fusion segments and avoid the increased stress of adjacent segment which can ensure a good preliminary clinical outcome.

Distalización con el C-DFD modificado con mini-tornillos. Reporte de caso / Distalization with a C-DFD modified with mini-screws. A case report

En la actualidad existen muchos dispositivos para distalizar los molares cuando se presenta una maloclusión clase II; como son la tracción extraoral, el péndulo dento y óseosoprtado, el distal jet, sliding jig entre otros. Todos con efectos secundarios indeseables. Este reporte de caso pretende ilustrar los resultados obtenidos con un dispositivo con anclaje cortical y óseo en una maloclusión clase II. Reporte de caso clínico en un paciente clase II esquelético y dental. Se usó un aparato de anclaje cortical, diseñado en la Universidad CES de Medellín, llamado Cortical Dual Forcé Distalizer (C-DFD), el cual fue modificado con un par de mini-tornillos para reforzar el anclaje, logrando la distalización superior de los molares evitando un tratamiento con exodoncias. El C-DFD es un distalizador óseo-soportado que logra un movimiento distal del primer molar superior.

There are currently many devices used for molar distalization when there is a Class II malocclusion; some of these devices include headgear, tooth-supported and bone-supported pendulums, the distal jet, and the sliding jig, among others. All of them have undesirable side effects. This case report aims to illustrate the results obtained by using a device with cortical and bone anchorage to treat a Class II malocclusion of a patient with a skeletal and dental class II malocclusion. A Cortical Dual Forcé Distalizer (C-DFD, a cortical-anchoring appliance designed at CES University in Medellín, Colombia was used. This device was modified by using a couple of mini-screws to reinforce anchorage, thus achieving upper distalization of molars and avoiding treatment with extractions. The C-DFD is a bone-supported distalizer that achieves a more controlled and in-body distal movement of the first upper molar, avoiding the inclinations produced by other distalizers.

Acoso en el ambiente escolar: análisis de un cuestionario mediante Teoría de Respuesta al Ítem y Análisis de Correspondencias Múltiples / Analysis of a School Bullying Questionnaire Using Item Response Theory and Multiple Correspondence Analysis

En este estudio se reanalizaron los datos de Cepeda C., Pacheco Durán, García Barco y Piraquive Peña (2008) mediante un modelo de Teoría de Respuesta al Ítem (TRI), y mediante Análisis de Correspondencias Múltiples (ACM) y de clasificación. Se encontró que las propiedades psicométricas del cuestionario de acoso escolar son bastante buenas (α = 0.96 y buen ajuste al modelo unidimensional a partir del análisis paralelo). Además, se presentan las coincidencias entre los resultados de los dos tipos de análisis, y se encuentran resultados similares a otros estudios en Colombia. Los resultados de este estudio indican que el cuestionario analizado es una medida válida y confiable del acoso en el contexto escolar.

This study presents reanalyzes of Cepeda et al. (2008)'s data using an Item Response Theory (IRT) model and Multiple Correspondence Analysis (MCA). Both internal consistency (α = 0.96) and fit to the unidimensionality assumption (per parallel analysis) were found to be satisfactory. Coincidences between both analyses are shown. The percentage of students affected by bullying in the sample is similar to that found in other studies in Colombia. Finally, the questionnaire appears to be a valid and reliable measure of bullying.

The detection and significance of AKAP12 methylation levels in peripheral blood in patients with lung cancer / 重庆医学

Objective To detect the levels of AKAP12 methylation by methylation‐specific high‐resolution melting curve(MS‐HRM ) in peripheral blood in patients with colorectal cancer and investigate its clinical significance .Methods We used MS‐HRM technology to detect the levels of AKAP12 methylation in peripheral blood in 60 lung cancer patients ,and analyzed the relationship between the levels of AKAP12 methylation and pathological parameters of lung cancer patients .Results 34(56 .7% ) of the 60 lung cancer patients were found to be methylated at the AKAP12 promoter region by MS‐HRM ,the methylation levels of 18 cases ranged between 1% -20% ,14 cases ranged between 20% -60% ,2 cases ranged between 60% -100% .There was no significant differences between the levels of AKAP12 methylation and lung cancer patients′age and gender(P>0 .05) .However ,it was signifi‐cantly higher in the patients with high pathological stage and differentiation degree (P<0 .05) .Conclusion AKAP12 promoter re‐gion methylation was related to tumor progression and malignant degree .

Single-Port Thoracic Surgery: A New Direction

Single-port video-assisted thoracic surgery (VATS) has slowly established itself as an alternate surgical approach for the treatment of an increasingly wide range of thoracic conditions. The potential benefits of fewer surgical incisions, better cosmesis, and less postoperative pain and paraesthesia have led to the technique's popularity worldwide. The limited single small incision through which the surgeon has to operate poses challenges that are slowly being addressed by improvements in instrument design. Of note, instruments and video-camera systems that are narrower and angulated have made single-port VATS major lung resection easier to perform and learn. In the future, we may see the development of subcostal or embryonic natural orifice translumenal endoscopic surgery access, evolution in anaesthesia strategies, and cross-discipline imaging-assisted lesion localization for single-port VATS procedures.