RESUMO
The study was aimed to evaluate the analgesic and anti-inflammatory activity (by both in-vitro and in-vivo) of both chloroform and methanol root extracts of Andrographis serpyllifolia (Rottl. Ex Vahl.) Wt. Methods used for the studies were In-vitro 5-Lipoxygenase inhibition assay and In-vivo measurement of rat paw edema and ear edema in rats, acetic acid induced writhing response and hot plate method in albino mice. Chloroform and methanolic extracts of A. serpyllifolia root have shown moderate potency in inhibiting 5-LOX and shown significant anti-inflammatory activity. Despite the IC50 values are little higher, anti-inflammatory efficacy of these extracts possibly due to other mechanisms apart of 5-LOX inhibition. However, In-vivo anti-inflammatory studies revealed that A. serpyllifolia methanolic extract has shown higher degree of efficacy when compared to the chloroform extract. In terms of analgesic activity in writhing test, methanolic extract has shown more efficacy than chloroform extract. Hence, it is important to isolate the active principles for further testing the anti-inflammatory efficacy.
RESUMO
The study was aimed to evaluate the analgesic and anti-inflammatory activity (by both in-vitro and in-vivo) of both chloroform and methanol root extracts of Andrographis serpyllifolia (Rottl. Ex Vahl.) Wt. Methods used for the studies were In-vitro 5-Lipoxygenase inhibition assay and In-vivo measurement of rat paw edema and ear edema in rats, acetic acid induced writhing response and hot plate method in albino mice. Chloroform and methanolic extracts of A. serpyllifolia root have shown moderate potency in inhibiting 5-LOX and shown significant anti-inflammatory activity. Despite the IC50 values are little higher, anti-inflammatory efficacy of these extracts possibly due to other mechanisms apart of 5-LOX inhibition. However, In-vivo anti-inflammatory studies revealed that A. serpyllifolia methanolic extract has shown higher degree of efficacy when compared to the chloroform extract. In terms of analgesic activity in writhing test, methanolic extract has shown more efficacy than chloroform extract. Hence, it is important to isolate the active principles for further testing the anti-inflammatory efficacy.
RESUMO
The present study was carried out to evaluate the antimicrobial activity of the crude methanolic extracts of Memecylon malabaricum Clarke. (leaves), Cochlospermum religiosum Linn. (leaves and flowers) and Andrographis serpyllifolia Vahl. (leaves) using the standard disc diffusion assay against eight strains of bacterial species, viz., Staphylococcus aureus, Salmonella typhi, Enterobacter aerogenes, Pseudomonas aeruginosa, Xanthomonas oryzae pv. oryzae, Xanthomonas axonopodis pv. malvacearum, Bacillus cereus and Micrococcus sp. The extracts of the plants at a concentration of 1.25 mg/disc showed minimum to moderate activity against both Gram positive and Gram negative bacteria indicating a broad spectrum activity. A preliminary phytochemical screening was conducted on the selected plant extracts using standard qualitative procedures that revealed the presence of several secondary metabolites. The extracts failed to show antioxidant activity by reducing power assay. The result indicates the potential usefulness of these plants especially Memecylon malabaricum and Cochlospermum religiosum, in treating microbial infections in humans and plants and justifies the need for further investigations and characterization of the bioactive compounds present in the methanolic extracts of the plants.