RESUMO
Objective To study the chemical constituents from the whole plant of Anisomeles indica. Methods The constituents were isolated and purified by various column chromatographic techniques including silica gel, Sephadex LH-20, and ODS. The structures were identified by spectroscopic analyses and physicochemical properties. Results Ten compounds were isolated from the ethanol extract of the whole plant of Anisomeles indica, in which seven phenylethanoids were identified as epimeredinoside A (1), crenatoside (2), verbascoside (3), cistanoside D (4), 3'-O-methyl isocrenatoside (5), isocrenatoside (6), and glycosmisic acid (7). Three phenolic acids were vanillic acid (8), caffeic acid (9), and ferulic acid (10). Conclusion Compounds 4-7 are isolated from the title plant and Anisomeles genus for the first time.
RESUMO
The chloroform (4.20% w/w), ethyl acetate (4.23% w/w) and aqueous decoction (12.11% w/w) extracts of the aerial parts of A. indica were screened for the antiepileptic activity against maximal electroshock (MES) model and pentylenetetrazole (PTZ) models at doses of 200, 400 mg/kg, po once. Phenytoin and diazepam (25 and 2 mg/kg, ip) were used as standard drugs in MES and PTZ model, respectively. Further, ethyl acetate extract (active extract) was fractionated into flavonoid and tannin fraction, which were subsequently evaluated for the antiepileptic potential against both MES and PTZ models at a dose of 50 mg/kg, po. Pretreatment with ethyl acetate extract 200, 400 mg/kg, po, for 1 week showed significant antiepileptic activity against PTZ induced convulsions only. Isolated flavonoid fraction showed more potent antiepileptic activity as compared to ethyl acetate extract, without any neurotoxic effect. However, tannin fraction did not produce antiepileptic activity against PTZ induced convulsions. It may be concluded that the flavonoids fraction of ethyl acetate extract of aerial parts of A. indica, but not the aqueous decoction has antiepileptic potential, without producing neurotoxic effects.