RESUMO
OBJECTIVE:To establish a method for the determination of antiseptics in Anshen bunao oral liquid. METHODS:HPLC method was adopted. The determination was performed on Agilent Zorbax SB-C18 column with mobile phase consisted of ace-tonitrile-0.05% phosphorice acid solution (gradient elution) at the flow rate of 1.0 mL/min. The detection wavelength was set at 236 nm,and column temperature was 30℃. The injection volume was 10μL. RESULTS:The linear range of benzoic acid and eth-ylparaben were 12.85-128.54 μg/mL(r=0.9999)and 4.05-40.48 μg/mL(r=0.9998),respectively. The limits of quantitation were 0.514,1.012 μg/mL. The limits of detection were 0.171,0.353 μg/mL. RSDs of precision,stability and reproducibility tests were all lower than 1.0%. The recoveries were 99.38%-100.21%(RSD=0.31%,n=6),97.96%-100.72%(RSD=0.91%,n=6). CON-CLUSIONS:The method is simple and accurate,and can be used for content determination of antiseptics in Anshen bunao oral liq-uid.