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Objective To observe the clinical efficacy of Ziyin Yikang Recipe plus hexadecadrol on anti-HCG antibody positive secondary sterility and the influence on anti-HCG antibody titers. Methods Sixty patients with anti-HCG antibody positive secondary sterility were randomly divided into treatment group (TG) and control group (CG), 30 cases in each. Patients in the TG were treated with Ziyin Yikang Recipe plus hexadecadrol, while patients in the CG were given hexadecadrol only. The Chinese medicine symptom scores and changes of anti-HCG antibody titers were evaluated and compared between the two groups.Results The Chinese medicine symptom scores in the two groups decreased compared with those before treatment (P0.05). The total effective rate in the TG was higher that that in the CG (P<0.05), with 63.33% (19/30) in the TG and 47.66% (14/30) in the CG.Conclusion The treatment of Ziyin Yikang Recipe plus hexadecadrol on anti-HCG positive secondary sterility can obviously improve the clinical syndrome of yin deficiency and fire hyperactivity, and promote pregnancy success.
RESUMO
Objective:In the past study, a plasmid of pcDNA3 hCG? C3d3 had been constructed. It was suggested that the fusing protein be expressed by the pcDNA3 hCG? C3d3 plasmid both in the transient expression system in COS 7 cells, and the stable expression system in CHO cells. In the present research, it would be testified that the C3d molecular adjuvant could improve the immunogenicity of the hCG? DNA immunization or not. Methods:BALB/C mice of 6 weeks old were immunized intramuscularly two times at interval of 3 weeks by the plasmid pcDNA3, pcDNA3 hCG?, pcDNA3 hCG? C3d3 at dosage of 5, 10, 20 pmol, respectively. The anti hCG? antibody titers were determined by indirect ELISA in 6 weeks of last immunization. Results:The result showed that the C3d molecular adjuvant could increase significantly the titer of anti hCG? antibody in a dose dependent manner after DNA immunization.Conclusion:The C3d molecular adjuvant does improve the humoral immunity of the hCG? DNA immunization, which would be helpful for technical progress and clinical trial of the contraceptive vaccine. [
RESUMO
The product of a hybrid cell clone, P3W80, obtained as ascites fluid from mouse peritoneal cavity had high titres of anti human chorionic gonadotropin antibodies e.g. 30 to 40% binding of 125I human chorionic gonadotropin at 107dilution in a radioimmunoassay. The antiserum SB6 (raised against ß human chorionic gonadotropin distributed by National Institutes of Health, USA gave similar binding at 5000 dilution in parallel runs. The monoclonal antibody recognized best human chorionic gonadotropin (0.3 mIU of hormone/tube with B/Bo <75%), but also bound ß and α subunits of human chorionic gonadotropin, 12 and 800 folds lower than human chorionic gonadotropin respectively. No binding was observed with car boxy terminal peptides of β human chorionic gonadotropin ranging from 93 to 145 amino acid residues, indicating the lack of recognition of the C terminal region. No cross reaction with human leutinizing hormone was obtained at the physiological surge levels, a significant competition (B/B0 <75%, obtainable only at 60 mIU of LER 960 human leutinizing hormone/ tube. T h e antibody had heavy chain of IgG1 and light chain of kappa type. It neutralized the bio activity of human chorionic gonadotropin both in vitro and in vivo.