Soroepidemiologia de anticorpos igg (imunoglobina g) anti helicobacter pylori em universitários / Seroepidemiology of antibodies igg (immunoglobulin g) anti-helicobacter pylori at university

Objetivo: caracterização do perfil sorológico de anticorpos anti-H. pylori da classe IgG (Imunoglobulina G) de amostras de soro de estudantes universitários, a fim de verificar a soroprevalência de anticorpos anti-H. pylori em universitários de uma instituição privada do interior de São Paulo. Método: a técnica a ser empregada refere-se à técnica de ImmunoComb H. Pylori IgG (ELISA) comercial da empresa Orgenics. Resultados: foram analisadas 90 amostras de estudantes voluntários, sendo 45 do sexo masculino. Verificou-se que 75,5% (68/90) amostras analisadas eram soropositivas para IgG anti-H. pylori, sendo 86,6% (39/45) do sexo feminino e 64,4% (29/45) do sexo masculino. Conclusões: estes resultados indicam alta prevalência de anticorpos anti-H. pylori, predominantemente para o sexo feminino, quando comparado com as amostras do sexo masculi

Objective: to characterize the serological profile of anti-H. pylori IgG (Immunoglobulin G) of degree students samples at private university to determine the seroprevalence of antibodies anti-H. pylori. Methods: Therefore, the technique to be used refers to ImmunoComb H. pylori IgG technique (ELISA) of Orgenics company's. Results: 90 samples of students were analyzed with 45 male samples. It was found that 75,5% (68/90) samples were seropositive for IgG anti-H. pylori, with 86,6% (39/45) female and 64,4% (29/45) male. Conclusions: these results indicated a high prevalence of IgG anti-H. pylori predominantly in females compared with male samples.

Falha na sexagem por inibição do desenvolvimento de embriões bovinos produzidos in vitro com anticorpos anti H-Y / Failure of sexing by developmental arrest of bovine embryos in vitro produced with H-Y antisera

Embriões bovinos produzidos in vitro, em estádio de mórula, foram cultivados em meio contendo anticorpos anti H-Y de alto título proveniente de ratos por 24h e, após este tempo, classificados em dois grupos: 1) embriões inibidos em estádio de mórula (classificados como machos) e 2) embriões que se desenvolveram e formaram a blastocele (classificados como fêmeas). O sexo de 311 embriões, distribuídos em três grupos de concentração dos anticorpos, 3 por cento, 5 por cento ou 7 por cento, foi identificado pela reação em cadeia da polimerase. Não houve desvio da proporção entre machos e fêmeas (P>0,05) nos grupos em que se utilizaram os anticorpos anti H-Y, quando comparadas ao grupo-controle, sem adição de anticorpos anti H-Y. Diferentemente dos resultados obtidos utilizando-se embriões bovinos produzidos in vivo, a sexagem com anticorpos anti H-Y de alto título em embriões produzidos in vitro não propiciou sucesso.

In vitro produced bovine embryos at morula stage were cultured in medium containing high titer of rat H-Y antisera for 24h. The embryos were classified in two groups: 1) embryos arrested at morula stage (classified as males); and 2) embryos that developed and formed a blastocoele (classified as female). The sex of 311 embryos, divided in three groups of concentration of H-Y antisera, 3 percent, 5 percent or 7 percent, was identified by polimerase chain reaction. The results showed no difference (P>0.05) on sexual deviation in groups in which the H-Y antisera was added, in relation to control group, in which no H-Y antisera was added. In contrast with results obtained with in vivo produced bovine embryos, the sexing of in vitro produced bovine embryos with high H-Y antisera titer did not succed.

Preparation and determination of polyclonal immune milk to H pylori / 中国免疫学杂志

Objective:To prepare anti-H pylori immune milk to prevent the infection of H pylori.Methods:The whole active H pylori as immunogen, cows were immunized and the polyclonal immune milk to H pylori was obtained. Results: With direct agglutination test and doubled agar diffusion detection, the titer of antibody was 1:2 048 and 1:32.Conclusion: The prepared immune milk against H pylori has good polyclonal specificity and high antibody titeration.

Comparison of Different Methods for the Diagnosis of Helicobacter pylori Infection: Histology, CLO test, Culture, Serologic test and PCR / 대한임상미생물학회지

BACKGROUNDS: Helicobacter pylori infection is now recognized as a cause of chronic gastritis, peptic ulcer disease, gastric carcinoma and lymphoma. Several diagnostic methods of H. pylori infection, such as histopathology, culture, rapid urease test, urea breath test, serologic test and polymerase chain reaction(PCR) have been used. This study aimed to compared with different diagnostic methods of H. pylori infection and determined the appropriate cut-off value of IgG anti-H. pylori antibody using receiver operating characteristic(ROC) curve. METHODS: We compared sensitivities, specificities and efficiencies of histology, CLO test, culture, IgG anti-H. pylori Ab and PCR using the ureC gene in gastric biopsy specimens from 112 H. pylori patients and 140 control group. RESULTS: The sensitivities of histology, CLO test, culture, IgG anti-H. pylori Ab and PCR were 72%, 91%, 86%, 82% and 94%, respectively and the specificities of histology, CLO test, culture, IgG anti-H. pylori Ab and PCR were 96%, 99%, 100%, 73% and 99%, respectively. The efficiencies of histology, CLO test, culture, IgG anti-H. pylori Ab and PCR were 88%, 96%, 89%, 77% and 97%, respectively. From the ROC curve, the cut-off value of the anti-H. pylori Ab determined 10U/mL in which sensitivity was 82% and specificity was 82%. CONCLUSIONS: These findings suggest that the PCR assay in gastric biopsy is the most sensitive and efficient diagnostic method of H. pylori infection and the cut-off value of the anti-H. pylori Ab determines 10U/mL showing highest efficiency.

Identification of the para-Bombay phenotype AB h m / 中国输血杂志

Objective To identify para-Bombay phenotype AB h m. Method ABO and H phenotype were typed. Absorption and elution were performed. Saliva was tested by inhibitory reaction. Direct sequencing was performed and family study was done. Results Proband was typed as rare para-Bombay phenotype AB h mand anti-H was detected in his serum. Family study suggested that the inheritance was autosomal recessive. Conclusion Rare AB h m phenotype was identified and anti-H has been detected in his serum.