Analysis of three serological tests results of hemolytic disease of newborn in 1 350 cases / 中国免疫学杂志

Objective:To explore blood type distribution of newborns hemolytic disease ( HDN ) caused by maternal and neonatal blood type incompatibility and analyze the value of hemolysis three trials in the diagnosis of HDN.Methods:Hemolysis three trials of type O or Rh negative maternal cord blood samples and hyperbilirubinemia of the newborn blood samples from January 2014 to 2016 were detected by micro-column gel test cards.Then the results were statistically analyzed.Results:(1) There were 918 cases of maternal and neonatal blood type incompatibility in all 1350 cases.569 cases were detected HDN positive with the rate of 62%( 569/918).Among 569 cases,the positive rate of direct anti-globulin test,free antibody test and antibody released test were 27.9%(159/569),86.5%(492/569) and 100% respectively.There was statistical difference of the combination of direct anti-globulin test negative,free antibody test positive and antibody released test positive compared with other combinations ( P<0.05 ).( 2 ) There was statistical difference of HDN positive rate between ABO 73.8%(551/747) and Rh 10.5%(18/171)in 918 cases of blood type incom-patibility.(3)There was statistical difference between A positive rate of 80%(280/350) and B positive rate of 68.3%(271/397) in 747 cases of ABO incompatibility.(4)There was statistical difference among RhD positive rate of 17.7%(14/79),RhE positive rate of 6.8%(4/59) and RhC positive rate of 0(0/33).Conclusion: Antibody released test was the most sensitive test in hemolysis three trials to diagnose HDN.The probability of HDN positive caused by maternal and neonatal ABO blood type incompatibility was significantly higher than Rh.The probability of HDN positive with type A newborns was significantly higher than type B.The probability of HDN positive caused by RhD blood type incompatibility was significantly higher than RhE and RhC.

Study of the relationship between antibody release test and the morbidity of hyperbilirubinemia in newborns / 中国小儿急救医学

Objective To study the relationship between antibody-rdeased test and the morbidity of hyperbilirubinemia in newborns.Methods According to the laboratory rules,serological tests were practised with neonatal umbilical blood of 1 013 A or B blood-type neonates delivered by O-type blood mothers.The morbidity of hyperbilirubinemia were grouping studied in 7 days after birth tO the neonates according to test results.Results(1)The morbidity of hyperbilirubinemia neonates with positive antibody release test results was much higher than that of control group(P＜0.01).(2)Of the antibody release test positive results,there were significant differences among the three groups in which the degree of red cell agglutination were different (P＜0.01).(3)Of the antibody-released test positive results,there was no significant difference in the morbidity of hyperbihrubinemia between the neonates sensitized by the anti-A+B IgG and those not sensitized by the anti-A+B IgG(P＞0.05).There had significant difference in the morbidity of hyperbilirubinemia between the neonates with free antibody and those without free antibody(P＜0.01).Conclusion The different results of antibody release test play some role in the clinical judgement whether hyperbilirubinemia of newborns will occur.

Study on the early diagnosis of HDN in ABO blood-group system / 中国小儿急救医学

Objective To study the early diagnosis of heamolytic disease of newborn(HDN) by umbilical blood immunoserological test. Methods According to the laboratory rules, immunoserological tests were practised with neonatal umbilical blood of 916 A or B blood-type neonates delivered by O-type blood mothers. The morbidity and the pathogenic time of hyperbilirubinemia were studied in 7 days after birth to the neonates whose erythrocyte was sensitized by the anti-A(B) IgG. Results (1) There was significant difference in the morbidity of hyperbilirubinemia of the neonates whose umbilical blood antibody-released test results were postive compared with control group (P<0.01), There had no significant difference in the morbidity of hyperbilirubinemia of the neonates compared with puerperal times. (2) There had significant difference in the morbidity of hyperbilirubinemia between the neonates having free antibody and those without free antibody (P < 0.01). (3) The peek time of hyperbilirubinemia was in 1-3 days after birth in patient-infants whose antibodyreleased test result was positive. Conclusion The umbilical blood immunoserological test is an exact method of the early diagnosis to HDN. The free antibody helps to estimate the developing trend of HDN.

A discussion about the relationship between the different Ab-A(B) titer of the pregnant women and the morbidity of hyperbilirubinemia happened to the neonates / 中国基层医药

Objective To discuss the relationship between the titer of immunoglobulin Ab-A(B) in the O- type-blood pregnant women and the morbidity of hyperbilirubinemia which the A(B)-type-blood neonates developing. Methods The Ab-A(B) titer of the pregnant wives whose blood type was O and didn't match their husbands' was determined. Determine the serology with umbilical blood of the A(B)-type-blood neonates was determined, and the morbidity of hyperbilirubinemia which happened to those antibody-released test positive neonates was determined.Results (1)In the 271 A(B)-type-blood cases,when the pregnant woman's Ab-A(B) titer≥32,from low to high,the positive incidence of the antibody-released test and indirect antiglobulin test heightened remarkably(P