Clinical significance of antiendothelial cell antibody in systemic vasculitis and their autoantigens / 基础医学与临床

Objective To investigate the prevalence,their autoantigen and the clinical significants of antiendothelial cell antibodies(AECAs) in systemic vasculitis.Methods Western blotting was performed to detect specific AECA in serum of systemic vasculitis,SLE,RA,SS and healthy donors.Then to analyze the relationships of AECA with the disease manifestation.Results(1)The prevalence of AECA was 77.7% in systemic vasculitis,87.5% in SLE,66.7% in SS,7.14% in RA and 10% in normal group respectively.(2)AECA reacted with a heterogeneous series of endothelial proteins which ranged in molecular size from 16 to 120 ku Furthermore,AECA against a 47 ku endothelial cell antigen were more frequently found in a variety of systemic vasculitis and SLE.(3)Compared with those in AECA-negative patients,the mean levels of ESR in AECA-positive patients with TA and the mean levels of BVAS in patients with WG,MPA and CSS were both significantly higher in AECA-positive patients.Patients with BD who have AECA against 47 ku endothelial cell proteins were more frequently found to have neuropathy than those 47 ku-AECA-negative patients,and the prevalence of inhanced CRP are also more frequent.Conclusion AECA showed to be correlated with the disease manifestation,and the same molecular sizeantigen could be found in a variety of systemic vasculitis and SLE.

Clinical Significance of Anti-endothelial Cell Antibody in Renal Transplant Recipients

In order to evaluate the role of anti-endothelial cell antibody (AECA) in acute rejection in renal transplantation, serum AECA IgG titers were measured in 68 healthy controls, 111 chronic hemodialysis (HD) patients and 58 first renal transplant recipients. The AECA titer in hemodialysis patients was higher than in healthy controls (13.9+/-5.0 vs. 4.8+/-2.3 U/mL, p0.05). After renal transplantation, AECA titer was decreased significantly (vs. 4.7+/-3.6 U/mL, p<0.01). The serum AECA IgG titers increased significantly in recipients with acute rejection (6.9+/-3.1 vs. 13.5+/-9.9 U/mL, p<0.01), but decreased to 5.6+/-3.0 U/mL (p<0.01) after formal rejection therapy. In the recipients with acute rejection (n=27), the pre-renal transplant AECA titer was higher than in that without acute rejection (14.0+/-8.6 vs. 7.7+/-3.8 U/mL, p<0.01). The results of this study lead us to conclude that pre- and post-renal transplant AECA titer might be a useful predictor for acute rejection and useful for monitoring acute rejection in renal transplant recipients.

The Analysis of Anti-Endothelial Cell Antibody Expression in the Sera of Buerger's Disease Patients Using Enzyme-Linked Immunosorbent Assay

PURPOSE: The pathogenesis of Buerger's disease is unknown and only smoking is considered as an important factor of the occurrence and recurrence of the disease. The development of antiendothelial cell antibody (AECA), a kind of autoantibody against the vascular endothelium, is recognized as a common serological feature in several diseases characterized by immune-mediated vascular damage. The purpose of this study is whether AECAs develop in the sera of patients of Buerger's disease or not and, if develop, to find what relationship exists between the status of the disease and the degree of AECA expression. METHODS: Twenty sera were obtained from 15 patients with Buerger's disease, and 11 from 11 healthy male smokers as controls. Additionally 5 sera from nonsmoking females and 4 from nonsmoking males were tested. Among 5 out of 15 Buerger's disease patients, the blood samples were obtained twice. Statistically there was no age difference between patients and controls. Human umbilical cord vein endothelial cells (HUVEC) were cultured and used as the target of AECA. The expression of AECA was measured with enzyme-linked immunosorbent assay (ELISA) and anti-human peroxidase conjugated goat IgG was used as a secondary antibody. RESULTS: The value of whole patient's group 666 60 vs. controls 88 10, there was statistically significant (P<0.05); a group of active disease status 797 61 vs. remission 423 60, higher in active group (P<0.05); smokers in patients 732 73 vs. quit-smokers 544 94, higher in smokers, but not significant; no difference was observed between healthy females and males regardless of smoking history. CONCLUSION: The AECA seems to be closely related with Buerger's disease and the measurement of AECA expression with ELISA may be considered as a useful tool for the diagnosis of Buerger's disease and as a helpful predictor of occurrence and relapse of the disease.

The Analysis of Anti-Endothelial Cell Antibody Expression in the Sera of Buerger's Disease Patients Using Enzyme-Linked Immunosorbent Assay

PURPOSE: The pathogenesis of Buerger's disease is unknown and only smoking is considered as an important factor of the occurrence and recurrence of the disease. The development of antiendothelial cell antibody (AECA), a kind of autoantibody against the vascular endothelium, is recognized as a common serological feature in several diseases characterized by immune-mediated vascular damage. The purpose of this study is whether AECAs develop in the sera of patients of Buerger's disease or not and, if develop, to find what relationship exists between the status of the disease and the degree of AECA expression. METHODS: Twenty sera were obtained from 15 patients with Buerger's disease, and 11 from 11 healthy male smokers as controls. Additionally 5 sera from nonsmoking females and 4 from nonsmoking males were tested. Among 5 out of 15 Buerger's disease patients, the blood samples were obtained twice. Statistically there was no age difference between patients and controls. Human umbilical cord vein endothelial cells (HUVEC) were cultured and used as the target of AECA. The expression of AECA was measured with enzyme-linked immunosorbent assay (ELISA) and anti-human peroxidase conjugated goat IgG was used as a secondary antibody. RESULTS: The value of whole patient's group 666 60 vs. controls 88 10, there was statistically significant (P<0.05); a group of active disease status 797 61 vs. remission 423 60, higher in active group (P<0.05); smokers in patients 732 73 vs. quit-smokers 544 94, higher in smokers, but not significant; no difference was observed between healthy females and males regardless of smoking history. CONCLUSION: The AECA seems to be closely related with Buerger's disease and the measurement of AECA expression with ELISA may be considered as a useful tool for the diagnosis of Buerger's disease and as a helpful predictor of occurrence and relapse of the disease.

Enhanced Binding of Antiendothelial Cell Antibody by IL-1alpha in Systemic Lupus Erythematosus / 대한류마티스학회지

Systemic lupus erythematosus(SLE) is a multiorgan disease which is associated with the occurrence of immune vascular damage. Antiendothelial cell antibodies(aECA) have been demonstrated in patients with SLE, and their presence could be related to vascular injury. Endothelial cell(EC) in vasculitic lesions of patients with SLE show evidence of activation. In vitro treatment of EC with cytokines can produce a pattern of activation such as the increased expression of surface molecules. Activation of EC was shown to influence the interaction between aECA and endothelial surface. The authors, therefore, examined the prevalence of IgG aECA and investigated the association of aECA with clinical findings and anticardiolipin antibodies(aCL). We also investigated the role of cytokine in binding of IgG-aECA to EC in patients with SLE. Sera from 92 patients with SLE and 20 healthy controls were assayed for IgG- aECA by cellular ELISA method using cultured human umbilical vein endothelial cell(HUVEC) and IgG-aCL by ELISA method, and We also examined the binding of IgG-aECA to EC activated with IL-1 or TNF-a< in sera from 60 patients with SLE. IgG-aECA was detected in the serum of 31 out of 92 patients(33.7%). The mean serum titers of IgG-aECA were significantly higher in SLE than in healthy controls(19.38 U/ml vs 8.79 U/ml, P<0.05). There was no correlation between serum titers of IgG-aECA and IgG-aCL(r=-0.0265). The mean serum titers of IgG-aECA in patients with digital vasculitis or Raynaud' s phenomenon were significantly increased in comparison with patients without these manifestations (35. 28 U/ml vs 18. 16 U/ml, P<0.05, 27.18 U/ml vs 15. 24 U/ml, P<0.05 respectively). The mean serum titers of IgG-aECA in 60 patients were significantly increased(P<0.0001) following the stimulation with IL-la as compared with unstimulated EC, but those of IgG-aECA were not increased following the stimulation with TNF-a<. The IgG-aECA binding reactivity to IL-la stimulated EC was significantly higher in patients with digital vasculitis, Raynaud s phenomenon, renal disorder, thrombosis, neuropsychiatric manifestation and avascular necrosis as compared with unstimulated EC. IgG-aECA was detected in 28 of the 60 patients(46. 7%). Its frequency was increased following the stimulation with IL-1<(68. 3%), but there was no significant difference in frequency following the stimulation with TNF-a<(48.3%). These data suggest that presence of IgG-aECA and enhanced binding of IgG- aECA to EC induced by IL-la< can play an important role in the occurrence of clinical manifestations associated with vascular damage in SLE.