Antipyretic and anti-inflammatory effects of Mosla chinensis based on integration processing technology of origin / 中草药

Objective To compare the effect of integration processing technology of origin with traditional cutting processing technology of Mosla chinensis on pyretic rats caused by LPS and inflammatory RAW264.7 cells induced by LPS, and provide the feasible basis for the implementation of integration processing technology of origin and traditional efficacy of the relieving exterior effects of M. chinensis. Methods The rats were intervened by M. chinensis (volatile oil, decoction), and the model of heat syndrome was established by ip of LPS. The temperature changes of the fever rats were observed. The levels of serum prostaglandin E2 (PGE2), tumor necrosis factor-α (TNF-α), adenosine cyclic phosphate (cAMP), interleukin-1β (IL-1β), and myeloperoxidase (MPO) in liver tissue were detected. The effects of integration processing of origin and traditional cutting processing of M. chinensis on heat syndrome rats were compared. The cell viability of LPS stimulated RAW264.7 macrophages cells after administration of different concentration of M. chinensis (decoction and volatile oil) was detected, and the NO secretion was determinated by ELISA for comparing the anti-inflammatory effect of two processing methods. Results The treatment of processed M. chinensis can decrease the anus temperature and the content of PGE2, TNF-α, CAMP, IL-1β, MPO, and enhance cellular immunity. Moreover the efficacy of integration processing of origin is better than traditional cutting processing, and the efficacy of M. chinensis decoction is better than volatile oil. Conclusion The integration processing technology of origin for M. chinensis is feasible.

Study on Antipyresis and Cholagogue Effect ofFructus Gardeniae - Fructus Forsythiae Herb Couple / 世界科学技术-中医药现代化

This study was aimed to observe the antipyresis and cholagogue effect of theFructus Gardeniae-Fructus Forsythiaeherb couple in order to explore the possible pharmacodynamic mechanism. Dry yeast suspension was subcutaneously injected into the back of rat to establish the fever model. The freeze-dried powders ofF. Gardeniae,F. Forsythiae, andF. Gardeniae-F. Forsythiaeherb couple were prepared. They were dissolved in the water for intragastric administration. SD rats were randomly divided into the normal group, model group, positive control group and Chinese medicine group. The Chinese medicine group was subdivided into the group ofF. Gardeniae,F. Forsythiae, andF. Gardeniae-F. Forsythiaeherb couple. The concentration of herbal water extract was 10 mL·kg-1 (which equaled to 3 g·kg-1 of a single crude herb). The concentration of positive control was 10 mL·kg-1. Intragastric administration of equal amount of normal saline was given to the blank group and the model group. Except rats in the normal group, rats in other groups were subcutaneously injected with 10 mL·kg-1 of 15% dry yeast suspension on the back of to establish the fever model. Electronic thermometer was used to record the body temperature of rats at 0, 1, 4, 5, 6, 7 and 8 h after the injection, respectively. Meanwhile, bile was collected from 1-1, 1-2, 2-4, 4-6, 6-8, 8-12, 12-24 h, respectively. Observation was given on changes of body temperature and bile amount of rat. The results showed thatF. Gardeniae,F. Forsythiae, andF. Gardeniae- F. Forsythiaeherb couple had certain effect to reduce the body temperature of rats. The temperature-reducing effect of the combination of both herbs was better than a single herb. TheF. Gardeniae -F. Forsythiae herb couple can reduce the body temperature of fever rat (P < 0.05, orP < 0.01). It had a little effect on the body temperature of normal rat. TheF. Gardeniae - F. Forsythiaeherb couple can promote the bile secretion, which was better than the single using of F. Gardeniae (P < 0.05). It was concluded that theF. Gardeniae - F. Forsythiaeherb couple had better temperature-reducing effect than the using of a single herb; however, there was no significant difference. But it had obvious effect on the promotion of bile secretion, which indicated the strengthening of cholagogue effect.

Pharmacology and Toxicology of Extract from Arcangelisia gusanlung / 中草药·英文版

Objective: To study the pharmacology and toxicology of the extracts from Arcangelisia gusanlung (EAG). Methods: The anti-inflammatory activities were investigated using various inflammatory models including ear edema induced by xylene in mice, paw edema induced by carrageenan, and cotton pellet granuloma in rats. The analgesic effect was observed in hot-plate test and writhing test in mice and the antipyretic effect was observed in rat fever model induced by yeast. The antitussive action was tested in mice by sequential method and expectorant action was evaluated by tracheal excretion of phenol red. The antidiarrhea function was observed on normal intestinal propulsion of mouse model of diarrhea induced by decoction of Sennae Folium. The toxicity was measured by toxicological experiment. Results: Each dose of EAG could significantly inhibit the paw edema, cotton pellet granuloma, and intestinal propulsion. EAG significantly reduced writhing times and amount of wet manure. Obvious antipyretic action to fevered rat was observed. EAG obviously increased the tracheal excretion of phenol red and prolonged the latency of cough. No toxic reaction was shown in the observed period, and the maximum tolerance dose of mice was equivalent to 1360 times of common-used dose in human. Conclusion: The clinical dosage of EAG is safe, and its anti-inflammatory, analgesia, antipyresis, antitussive, expectorant, and antidiarrhea effects are significant. © 2013 Tianjin Press of Chinese Herbal Medicines.

Efeito antipirético e tolerabilidade do ibuprofeno* versus a dipirona**, em dose oral única, em pacientes pediátricos - estudo aberto, randomizado, multicêntrico brasileiro / Antipyretic effects and tolerability of a single oral dose of ibuprofen versus dipyrone in children - an open, randomized, Brazilian multicenter study

Contexto e objetivo: A prática pediátrica se confronta constantemente com o dilema da febre e a ansiedade que esta gera nos pais e cuidadores. A escolha da droga antipirética é motivo de controvérsia. O presente estudo foi concebido para avaliar a atividade antipirética e a tolerabilidade de doses orais únicas de ibuprofeno versus dipirona em lactentes e crianças febris, no contexto da prática clínica diária. Delineamento do estudo: Estudo comparativo, multicêntrico, aberto e randomizado, conduzido em instituições hospitalares brasileiras. Métodos: Pacientes de ambos os sexos, entre 6 meses e 8 anos de idade, com temperatura axilar ³ 38,0oC, e início da febre entre 4 horas e 48 horas da entrada no estudo. A temperatura axilar foi classificada como baixa (entre 38,0°C e 39,1°C) e alta (> 39,1°C). Os pacientes em cada grupo foram igualmente randomizados (1:1) para ibuprofeno (10 mg/kg) ou dipirona (15 mg/kg), administrados em doses orais únicas. As avaliações foram realizadas após 10, 20, 30 e 45 minutos e, a seguir, de 1 em 1 hora, durante 8 horas após a administração. As variáveis primárias de eficácia do estudo foram as médias de temperatura a cada intervalo de tempo e a soma ponderada das diferenças de temperatura (SPDT) a partir dos valores iniciais ao longo de 4, 6 e 8 horas. Variáveis secundárias foram: tempo para normalização da temperatura (<37,2°C); persistência do efeito antipirético; eventos adversos; e impressão geral do tratamento. Resultados: Um total de 122 pacientes (54% sexo masculino), com idade média de 2,8 anos, foram randomizados para receber ibuprofeno (grupos de febre baixa [n = 42] e alta [n = 17]) ou dipirona (grupos de febre baixa [n = 43] e alta [n = 20]). As médias de temperatura foram signitivamente menores nos pacientes que receberam ibuprofeno, em relação aos que receberam dipirona, nos grupos de febre alta e baixa (p = 0,04). Após 1, 2 e 4 horas da administração das drogas, o valor absoluto da soma ponderada das diferenças de temperatura a partir dos valores basais foi significativamente menor no grupo de febre alta da dipirona, quando comparada ao grupo de febre alta do ibuprofeno, o que significa maior efeito para este último. Houve diferenças estatisticamente significativas no tempo para normalização da temperatura (<37,2°C) entre o ibuprofeno e a dipirona nos grupos de temperatura baixa (3,1 ± 2,04 vs. 4,5 ± 3,06 horas, p = 0,01) e alta (2,7 ± 1,68 vs. 5,4 ± 3,15 horas, p = 0,003). A diferença do tempo de persistência do efeito antipirético foi também estatisticamente significativa para o grupo de temperatura alta, a favor do ibuprofeno (3,4 ± 2,03 vs. 1,8 ± 1,89 hora, p = 0,01). As duas drogas apresentaram perfis de tolerabilidade comparáveis. Conclusões: Neste estudo pediátrico multicêntrico comparativo, aberto e randomizado de curto prazo, uma dose oral única de ibuprofeno demonstrou proporcionar antipirese mais rápida, potente e por um tempo mais longo do que uma dose oral única de dipirona, especialmente na presença de febre alta.

Experimental study on main pharmacodynamics of Kangjun Xiaoyan Tablets / 中成药

AIM: To study the mostly pharmacological effects of Kangjun Xiaoyan Tablets (Flos Lonicerae, Radix Stemonae, Radix et Rhizonia Rhei, etc.) METHODS: The pharmacological functions of KJXYT were observed by measuring its antibacterial, anti-inflammatory, antipyretic and immune etc. RESULTS: KJXYT had remarkably protected the mice from the infection with streptococcus pneumococcus and staphylococcus aureus; had dramatic anti-inflammatory effect on the inhibited feet edema of rat induced by egg white and the ear edema of mice induced by dimethylbenzene; and had obvious effect on fever of rats caused by dried yeast; besides, and strengthened the phagocytosis of mice’s reticuloendothelial system. CONCLUSION:KJXYT serves the function of antibiosis, anti-inflammation, antipyresis and immunpotentiation. enedth