RESUMO
Objective:To study the effect of resistance to CTL mediated cytotoxity of target cells transfected with Wee1 and treated with anti perforin antibody Methods:Cytotoxic T lymphocyte was obtained by mixing lymphocyte NIT culture in vitro,and proliferation index (PI) of lymphocytes was detected(MTT method) The recombinant vector pCMV Wee1Hu/GFP was transfected into mammalian cells NIT With the function of anti perforin antibody, NIT and NIT transfected with Wee1 used as target cells, CTL mediated cytotoxity was detected by LDH method Results:CTL proliferation was induced by mixing cell culture With anti perforin antibody, the cytolysis quantity of NIT transfected with Wee1 was least Conclusion:Target cells transfected with Wee1 could resist CTL mediated cytotoxity, and anti perforin antibody obviously increased the effect