RESUMO
Objective: To study the saponins from Reineckia carnea of Guizhou Miao medicine. Methods: The chemical constituents from 95% EtOH extract were isolated and purified by repeated silica gel column chromatography, macroporous resin, ODS C18, Sephadex LH-20 gel column chromatography, and Pre-HPLC, and their structures were identified on the basis of physical and chemical properties, HR-ESI-MS and NMR spectral analysis. Results: Ten compounds were isolated and identified as (1β,3β,16β,22S)-cholest-5-ene-1,3,16,22-tetrol1,16-di (β-D-glucopyranosede) (1), (1β,3β,16β,22S)-cholest-5-ene-1,3,16,22-tetrol-1-[O-α-L-rhamno-pyranosyl-(1→2)-β-D-glucopyranoside]-16-(β-D-glucopyranoside) (2), (20S,22R)-spirost-25(27)-en-1β,3β,4β,5β-tetraol-5-O-β-D-glucopyranoside (3), kitigenin 5-O-β-D-glucopyrannoside (4), aspidistrin A (5), (17,20-S-trans)-5β-pregn-16-en-1β,3β-diol-20-one-1-O-β-D-xylopyranosyl-(1→2)-[α-L-rhamnopyranosyl]-3-O-α-L-rhamnopyranoside (6), diosgenin-3-O-β-D-glucopyranoside (7), ophiopogonin T (8), prosaikogenin D (9), and saikosaponin b2 (10). Conclusion: Compounds 3, 5, 8, 9, and 10 are isolated from this plant for the first time, which provided some reference for further elucidation and development of saponins from R. carnea.
RESUMO
Objective To study the chemical components from the roots of Smilacina henryi. Methods Silica gel, Sephadex LH-20, and preparation of thin layer chromatography techniques were used for isolation, and 1D, 2D-NMR, IR, and HR-ESI-MS data were used for structure identification. Results Seven steroidal components were obtained and its chemical structures were elucidated as (25S)-5α-spirostan-9(11)-en-3β,17α-diol 3-O-β-D-glucopyranosyl-(1→2)-β-D-galactopyranoside (1), (25S)-5α-spirostan-9(11)-en- 3β,17α-diol (2), (25S)-5α-spirostan-9(11)-en-3β,17α-diol 3-O-β-D-glucopyranoside (3), diosgenin (4), aspidistrin (5), henryioside A (6), and henryioside B (7). Conclusion Compound 1 is a new steroidal saponin named henryioside D, and compounds 2-5 are isolated from the plant for the first time.