RESUMO
Objective To evaluate feasibility of a method for determining low and medium concentrations water iodine by an automatic iodine analyzer (hereinafter referred to as this method).Methods The low (0-10 μg/L)and medium (0-100 μg/L) concentrations water iodine were determined by an automatic iodine analyzer.Methodological experiments were carried out on this method,including standard curve linearity,detection limit,precision experiment,standard recovery experiment,and standard substance determination.Results The absolute values of the linear correlation coefficients of the low and medium concentrations standard curves were ＞ 0.999 0,and the detection limits were 0.32 and 2.60 μg/L,respectively.Precision:the coefficient of variations were all below 2％ (n =18) of low,medium and high iodine concentrations water samples in the range of low and medium concentrations water iodine,and the average recovery rates were 100.7％ and 101.1％ (n =6),respectively.The determination results of the water iodine standard substance GBW 09113d and GBW 09114d were (8.3 ± 0.3)and (61 ± 2) μg/L (n =6),respectively,which were within the given standard values range.Conclusions This method has higher precision and accuracy,less reagent consumption,less time consumption,and simple operation.It is convenient for large-scale detection,and has strong applicability.
RESUMO
Objective To evaluate a method for determining the iodine content in urine by an automatic iodine analyzer.Methods The iodine content in urine was determined by an automatic iodine analyzer.Methodological experiments were carried out on the method (including test standard curve linearity and detection limit,precision test,spike recovery test,standard substance determination experiment,method comparison test).The urine samples from the method comparison were obtained from 27 pregnant women collected from Nantantou Village,Baoshan Street,Licheng District,Jinan City,Shandong Province.and the results of the same samples were compared with the results determined by arsenic-cerium catalytic spectrophotometry (GB method).Results The absolute value of correlation coefficient of the mass concentration range (0-300 pg/L) standard curve was ＞ 0.999 0,the detection limit was 2.9 μg/L (the sample volume was 0.25 ml).Precision:the coefficient of variations of the three iodine concentrations in the range of low,medium and high were 3.8％,3.1％,2.3％ (n =18),respectively.Accuracy:the average recoveries were 98.8％,99.5％,101.2％ of low,medium and high urine samples in the range of curves (n =6),and the total average recovery was 99.8％.The measurement results of two urinary iodine standard substance were in the standard value range.Methods comparison experiment:there was no significant difference between the results of 27 urine samples measured by two methods (t =0.40,P ＞ 0.05).Conclusions The method of automatic iodine analyzer in detection of urinary iodine has higher precision and accuracy,less reagent dosage,and simple operation.It is suitable for detection of large quantities of urinary iodine samples.