RESUMO
Objective To investigate the expression of C/EBP homology protein(CHOP) in rat brain tissue after focal cerebral ischemia-reperfusion,as well as to observe the influence of the shenxiong injection on the expression of CHOP.Methods One hundred forty four male rats were randomly divided into three groups:the sham-operation group,operation group,shenxiong group.Focal cerebral ischemia and reperfusion rat models were established by using suture.Zea Longa method was introduced to evaluate neurologic behavioral changes.The levels of mRNA and protein of CHOP were measured with methods of immunohistochemistry and reverse transcription polymerase chain reaction.The neuronal apoptosis was detected by the method of terminal deoxynucleotidy1 transferase-mediated dUTP-biotin nick end labeling.Results The expression of CHOP was up-regulated in per-infarction after cerebral ischemia-reperfusion in rats.CHOP mRNA and protein levels peaked at the 12th h and 24th h after reperfusion,respectively.The apoptosis cell counting increased gradually after cerebral ischemia-reperfusion and peaked at the 24th h after reperfusion.Compared with the saline control group,treatment with shenxiong injection could reduce the neuronal apoptosis at the 6th,12th,24th,and 72nd h after reperfusion (P ＜0.01).Compared with the saline control group,treatment with shenxiong injection could decrease CHOP mRNA and protein expression at the 6th,12th,24th,and 72nd h after reperfusion (P ＜0.01).Conclusions Shenxiong Injection may prevent neurocyte from apoptosis by inhibition of the expression of CHOP induced by endoplasmic reticulum stress.
RESUMO
[Objective] To observe the apoptosis of cortical cells and expression of related gene bcl-2 in hippocampal gyrus and frontal lobe of rats with incomplete cerebral ischemia (ICI) and to investigate the effects of Yiqi Huoxue Tablet (YHT, mainly composed of Radix Astragali , Rhizoma Chuanxiong, Radix Angelicae Sinensis, Herba Leonuri, Rhizoma Acori Tatarinowii, Scorpio, etc) . [ Methods ] Rats were randomized into six groups: model group, mimic operation group, nimodipine group and low-dose, moderate-dose and high-dose YHT groups. Flow cytometry was used to detect the apoptotic rate and bcl-2 protein content in cortical cells. Histological changes were also observed under light microscope. [Results] Early apoptotic changes were found in the model group and the apoptotic rate and bcl-2 protein content were higher than those in the mimic operation group; after treatment of YHT, the apoptotic rate in frontal lobe was decreased as compared with that in the model group and that in hippocampal gyrus remained higher than that in the mimic operation group and bcl-2 protein content was reduced to the level of the mimic operation group. [ Conclusion ] ICI is related to the apoptosis of nerve cells and YHT has been proved an effectual drug for the apoptosis.