RESUMO
Objective To investigate changes in intestinal bacteria in chronic renal failure (CRF),their diagnostic value for CRF,and correlations between specific bacterial genera and renal function.Methods Fecal specimens were collected from 56 patients with CRF and 38 healthy controls in the Nephrology Department and Medical Examination Center of Shanxi People's Hospital between August 2017 and January 2018.High-throughput sequencing analysis of 16S rDNA V3-V4 hypervariable regions was performed for intestinal bacteria.Intestinal bacteria in CRF patients and healthy subjects were analyzed for alpha,beta diversity,species composition analysis,and differential species analysis.The diagnostic value of the presence of specific intestinal bacteria for CRF was analyzed using a receiver operating characteristic curve (ROC).Pearson's correlation analysis was used to analyze the correlation between the presence of specific genera and the estimated glomerular filtration rate (eGFR).Results The alpha and beta diversity in the CRF group was different from that in the control group (P ＜ 0.05).At the phylum level,Verrucomicrobia were significantly less abundant in the CRF group than that in the control group (0.70％ vs 3.09％,P ＜ 0.001).The abundance of Actinobacteria was significantly greater in the CRF group than that in the control group (1.48％ vs 1.14％,P=0.036).At the genus level,the abundance of Akkermansia (0.96％ vs 3.90％),Parasutterella (0.47％ vs 0.93％),and Lactobacillus (0.07％ vs 0.48％) in the CRF group was significantly less than those in the control group (all P ＜ 0.01).The abundance of Alloprevotella (0.41％ vs 0.04％) and Clostridium Ⅳ (0.6％ vs 0.1％) was significantly greater than those in the control group (all P ＜ 0.05).The diagnostic value of CRF for the area under the ROC curve (AUC) for Akkermansia was 0.753,and that for Lactobacillus diagnostic value of CRF was 0.792.The combined AUC diagnostic value of CRF for detection of Akkermansia and Lactobacillus was 0.830,with high disease prediction value.Lactobacillus abundance was positively correlated with eGFR (R=0.29,P=0.029).Conclusions The diversity and structure of intestinal bacteria are altered in patients with CRF.The abundance of Akkermansia and Lactobacillus has diagnostic value for CRF.The abundance of Lactobacillus is positively correlated with eGFR.
RESUMO
In recent years, many researches that is related to waterborne diseases, especially diarrhea and water quality, safety, personal sanitation, hygiene have been conducting in developing country. Kharaa and Orkhon river are tributaries of the Selenge river basin and the many industries,agriculture, mining, provinces, soums are located in the near of Selenge river basin. Also Tuul,Kharaa and Orkhon river water is very polluted (Mongolian human Development Report 2010: Water and Development report).The aim of the research is to study correlation between to Kharaa and Orkhon river’s water pollution level and rate of gastrointestinal infectious diseases of people who live in the near of Selenge river basin.We analysed Kharaa and Orkhon river’s water quality and statistical data of gastrointestinal infectious diseases (such as dysentery, diarrhea, hepatitis A virus and others). Then we conducted correlation analysis between to river water pollution level and rate of intestinal infectious diseases. Kharaa and Orkhon river’s water was determined “less polluted” by physical, chemical andorganic indicators. But total number of bacteria was determined highly and Proteus vulgaris,Citrobacter freundi, Enterobacter agglomerans pathogens were detected in these river’s water. There were significant positive correlations between level of nitrit in river water and incidence of dysentery and diarrhea. (r=0.38, p=0.022; r=0.291, p=0.005).