The effect of bacterial cellulose membrane compared with collagen membrane on guided bone regeneration

PURPOSE: This study was to evaluate the effects of bacterial cellulose (BC) membranes as a barrier membrane on guided bone regeneration (GBR) in comparison with those of the resorbable collagen membranes. MATERIALS AND METHODS: BC membranes were fabricated using biomimetic technology. Surface properties were analyzed, Mechanical properties were measured, in vitro cell proliferation test were performed with NIH3T3 cells and in vivo study were performed with rat calvarial defect and histomorphometric analysis was done. The Mann-Whitney U test and the Wilcoxon signed rank test was used (alpha<.05). RESULTS: BC membrane showed significantly higher mechanical properties such as wet tensile strength than collagen membrane and represented a three-dimensional multilayered structure cross-linked by nano-fibers with 60 % porosity. In vitro study, cell adhesion and proliferation were observed on BC membrane. However, morphology of the cells was found to be less differentiated, and the cell proliferation rate was lower than those of the cells on collagen membrane. In vivo study, the grafted BC membrane did not induce inflammatory response, and maintained adequate space for bone regeneration. An amount of new bone formation in defect region loaded with BC membrane was significantly similar to that of collagen membrane application. CONCLUSION: BC membrane has potential to be used as a barrier membrane, and efficacy of the membrane on GBR is comparable to that of collagen membrane.

Utilização experimental de hidroxiapatita sintética em alvéolos dentários de gatos domésticos (Felis catus): estudo clínico, radiográfico e histomorfométrico / Experimental application of hydroxyapatite in feline (Felis catus) dental sockets: clinic, radiographic, histologic and histomorphometric studies

A fim de avaliar a resposta biológica da hidroxiapatita sintética (HAP-91) nos alvéolos de felinos domésticos, este biomaterial foi implantado após extração do terceiro pré-molar inferior direito em 12 gatos e mantida por meio de uma membrana de celulose bacteriana. No lado esquerdo, os alvéolos foram apenas recobertos com a membrana de celulose bacteriana, formando o grupo-controle. Observou-se, durante a avaliação clínica, que todos os animais voltaram a comer normalmente ração úmida, sem apresentarem sinais de dor ou desconforto após a recuperação anestésica. A cicatrização da ferida cirúrgica ocorreu de forma satisfatória, sendo que a membrana de celulose bacteriana evitou a saída precoce da hidroxiapatita. Radiograficamente, aos 50 dias, todos os animais apresentaram radiopacidade óssea homogênea em ambos os lados. À análise histomorfométrica, observou-se adiantamento do processo de reparo do osso alveolar nos oito primeiros dias do grupo-tratado quando comparado ao grupo-controle, bem como atraso aos 30 dias, porém, aos 50 dias, ambos os grupos apresentavam porcentagem de tecido ósseo semelhante e morfologicamente normal. Os resultados sugerem que a hidroxiapatita é biocompatível, integra-se ao tecido ósseo alveolar e pode ser utilizada em felinos.

The biocompatibility of a material depends on its characteristics, as well as the species and the environment recipient. In order to evaluate the biological response of the synthetic hydroxyapatite (HAP-91) in feline dental alveoli, it was implanted in the right inferior third premolar after extraction in 12 cats and maintained through a bacterial cellulose membrane. On the left side the alveoli was covered with a bacterial cellulose membrane (control group). During clinical evaluations it was observed that the animals started to eat after the anesthetic recovery time, without clinical signs of pain. There was a satisfactory cicatrization of the surgical wound and the bacterial cellulose membrane aided in repairing the gum, avoiding the loss of the hydroxyapatite. Radiographs taken 50 days post surgery presented homogeneous bone radiopacity on both sides. The histological and histomorfometrical analysis showed a positive progress of the alveolar repair in the first 8 days in the treated group when compared to the control group and a delay at 30 days, however at 50 days both presented a similar and morphologically normal percentage of bone tissue. These findings suggest that HAP-91 is biocompatible and integrates into the feline alveolar bone.