RESUMO
Objective. Asses the effect of supplementation with Humic substances (HS) over some innate immunity parameters (serum bactericidal activity, phagocytosis, bacterial agglutination, respiratory burst and lisozyme activity) in phase after fasting of layer hens. Materials and methods. 120 posfasting phase Hy Line Brown layer hens were taken which were distributed into four groups: The first and the second were supplemented with 0.1 and 0.2% of HS, respectively. The third group was supplemented with 0.25 mg/kg on levamisole hydrochloride and fourth group have no supplementation; during sixty days period. Blood samples were collected on 8th, 30th and 60th of experiment day. Results. The phagocytic index and respiratory burst increased significantly at day 30th in HS supplemented groups. Alike, serum bactericidal activity and lisozyme activity improved on 8 th day, nevertheless, changes were no evident latter. The bacterial agglutination was high in supplemented groups evaluated at everyone times. Conclusions. Results showed that HS behave as immunostimulant in the early phase after fasting layer hens.
Objetivos. Evaluar el efecto de las sustancias húmicas (SH) sobre algunos parámetros de la inmunidad innata (actividad bactericida del suero, fagocitosis, aglutinación bacteriana, explosión respiratoria y actividad de la lisozima) en la fase posmuda de gallinas ponedoras. Materiales y métodos. Se utilizaron 120 gallinas ponedoras Hy Line Brown en la fase de posmuda, las cuales fueron divididas en cuatro grupos: Los dos primeros fueron suplementados con 0.1 y 0.2% de SH respectivamente, el tercer grupo fue suplementado con 0.25 mg/kg de Clorhidrato de levamisol y el cuarto grupo control sin suplemento; durante un período de 60 días. Las muestras sanguíneas se tomaron los días 8, 30 y 60 del experimento. Resultados. El Índice fagocítico y la explosión respiratoria se incrementaron significativamente a partir del día 30 de suplementación con SH. De la misma manera, la actividad bactericida del suero y la actividad de la lisozima aumentaron al día 8; no obstante no se evidenciaron cambios posteriores. La aglutinación bacteriana fue significativamente mayor en los grupos suplementados en todos los tiempos evaluados. Conclusiones. Los resultados demuestran que las SH se comportan como agentes inmunoestimulantes en la fase temprana de la posmuda en gallinas ponedoras Hy Line Brown.
Assuntos
Aglutinação , Atividade Bactericida do Sangue , Muramidase , FagocitoseRESUMO
Os objetivos do presente trabalho foram avaliar se o Super Bonder" apresenta algum risco de veiculação de microorganismos em humanos e se possui alguma atividade bactericida ou bacteriostática para cocos gram-positivo. Foi testada a esterilidade de dez tubas do adesivo nos meios de cultivo BHI, agar sangue, agar Sabouraud com cloranfenicol e Mycosel. A provável ação bactericida ou bacteriostática do adesivo Super Bonder"', in vitro, foi verificada utilizando cepas de Staphylococcus aureus e staphylococcus epidermidir, semeadas em meio de cultura agar sangue e agar Mueller-Hinton. Nao foi observado crescimento de microrganismos nos meios de cultura contendo somente gotas de Super Bonder"'. No agar sangue, observou-se ausência de beta-hemólise e de crescimento bacteriano nas áreas de sobreposição do adesivo. Em agarMueller-Hinton, houve rarefação de colônias ao redor das gotas do cianoacrilato.Os dados deste trabalho mostram que a cola de cianoacrilato Super Bonder"' parece ser livre de contaminação por microrganismos e apresenta possfvel atividade bacteriostatica in vitro.
The purposes of the present study were to verify the human's risk of the Super Bondet microorganisms' diffusion and the probably biocide or bacteriostatic adhesive's activity for gram-positive cocci. The sterility of ten tubes of the adhesive was tested in BHI culture tube and blood-agar, agar-Sabouraud with cloranfenicol and Mycosel culture plates. The biocide or bacteriostatic activity of the Super Bondet adhesive, in vitro, was verified using cultures of Staphylococcus aureus and staphylococcus epidermidis,seeded on blood-agar and agar-Mueller-Hinton culture plates. No microorganisms' growth was observed in the culture plates containing only drops of the Super Bondet. In agar-Mueller-Hinton, zones with reduced number of colonies were noticed around the cyanoacrylate's drops. In blood-agar, absence of haemolysis-beta and bacterial growth were observed in the covered areas of the adhesive. According to the results of this investigation, the Super Bondet cyanoacrylate is free of microorganisms' diffusion and probably inhibits in vitro the bacterial growth.