Principal Component Analysis in Biometric, Pulp Quality and Anatomical Properties of Thronless Bamboo (Bambusa balcooa)

Aims: To estimate the impact, connection and association among the biometric attributes, pulping qualities and anatomical characters in Bambusa balcooa. Place and Duration of Study: The study was conducted across the agro climatic regions viz., North Eastern Zone, Northern Zone, Western Zone, Cauvery Delta Zone and Southern Zone of Tamil Nadu, India during 2017-2018. Methodology: The Principal Components Analysis (PCA) was examined to establish the numbers of clusters using Statistical Package for Social Studies (SPSS) version 16.0.1 software in order to identify the patterns of variation (PCA). The principal component analysis was computed using the equation PCA = Σa jXj. Results: The PCA separated into three cluster principal components among the nineteen parameters studied. Out of nineteen principal components generated, twelve principal components had contributed positively on pulp yield. Among these twelve traits, maximum contribution to the pulp yield was observed by the traits viz., numbers of culms, hollocellulose, kappa number, tear index, burst index, fibre wall thickness and vessel diameter with respect to Bambusa balcooa. Conclusion: The results showed some relationships between the biometric attributes, pulping qualities and anatomical characters in Bambusa balcooa. PCA was shown to be a useful tool for assessing the impact and connection for further research.

Isolation of total RNA from hard bamboo tissue rich in polyphenols and polysaccharides for gene expression studies

RNA isolation from hard and woody internodal bamboo (Bambusa balcooa) tissue is very difficult due to the presence of secondary metabolites, polysaccharides, and polyphenolics. These compounds often co-precipitate with isolated RNA and hinder downstream applications. We have developed an efficient, cost effective and reproducible RNA isolation method from hard tissue of bamboo internode. This protocol includes an additional organic solvent refinement steps to remove endogenous phenolic compounds and acidic phenol (pH 4.2) to critically stabilize RNA in extraction buffer. In addition to these, two 2M Lithium chloride washing steps were introduced to eliminate DNA and polysaccharides contamination. The RNA isolated from the present protocol was found to be superior, when compared to total RNA extracted by other available protocols. The A260/A280 absorption ratio of the isolated RNA was found ranging between 1.89-1.97. The integrity of 28S and 18S rRNA was highly satisfactory when analyzed in agarose denaturing gel. RNA was further used for RT PCR, northern hybridization, cDNA library and subtractive hybridization without any further refinement.