Relationship between HRCT expression and airway inflammation in patients with asthma airway remodeling / 中国生化药物杂志

Objective To study the assessment of airway inflammation in asthma patients with airway remodeling by high resolution spiral CT(HRCT).Methods Retrospective analysis on 60 cases of chronicity-persistent period asthma patients with induced sputum IL-4 test,airway mucosa biopsy,HRCT scan; while 20 patients only used HRCT scan as control group.To compare the difference of sputum IL-4,HRCT test of airway wall thickness(WT),wall area(WA)and airway wall relative area(WA％)in different degrees of asthma paitents.Results WA,WA％and WT were significantly increased in different degrees of asthma patients when compared to control group(P<0.05),there were significant difference among each group(P<0.05).WA％and WT were positively correlated with induced sputum IL-4.Conclusion HRCT can effectively evaluate the airway inflammation in airway remodeling of asthma patients,which can be used to evaluate the severity of asthma,treatment effect and follow-up observation.

An Ultrastructural Study on the Early Morphologic Changes of the Retina in Streptozotocin-induced Diabetic Rats

There have been many theories for pathogenesis of diabetic retinopathy. However,no one theory can explain all of the pathogenesis. That means many factors contribute to the pathogenesis of the diabetic retinopathy. Many studies have shown the retinal changes in long-term hyperglycemia, yet relatively few in short-term hyperglycemia. To study the changes of the retinal capillary and retinal pigment epithelium in diabetic retinopathy, we measured the thickness of the basement membrane of the retinal capil-lary endothelial cells in streptozotocin-induced diabetic rats. An morphological alterations of the retinal pigment epithelium were also examined on the 2 month-old diabetic rats. The measurement was made using 0.1 mm calibrated ruler overlaying the electron micrograph with a transparent plastic sheet on which 20 radiating lines were etched exactly 18 .apart. The SPSS and Instat program were used for statistical evaluation of the data. Comparing with the control, the increase of the thickness of the endothelial basement membrane at the 2 month-old diabetic rats was statistically significant (p<0.05). Cytoplasmic alterations such as vacuolization of capillary endothelium, changes in mitochondria and increasement of capillary endothelialcytoplasm were more prominent in diabetic rats than in control rats. Basal infoldings of retinal pigment epithelium appeared to be more frequent and deeper in diabetic rats than in control rats.

Ultrastructural Changes of The Retinal Capillary Basement Membrane in The Streptozotocin-Induced Diabetic Rats

Ultrastructural changes of the retinal capillary basement membrane in the streptozotocin-induced diabetic rats Cho, Hokyun, M.D., Lee, Dohyung, M.D. We studied the changes of the retinal capillary in diabetic retinopathy. We measured the thickness of the basement membrane of the retinal capillary endothelial cells and the capillary pericytes using streptozotocin-induced diabetic rats and the results were compared with that of normal control rats. And also an morphological lterations of the retinal capillary were examed on the prediabetes and 3, 7 month-aged diabetic rats. The basement membrane thickness was measeured with 0.1mm calibrated ruler overlaying the electron micrograph with a transparent plastic sheet on which were etched 20 radiating lines exactly 18degree apart. The SPSS and Instat program were used for statistical evaluation of the data. An age related increase of the basement membrane of the capillary pericyte and endothelial cell was evident in both diabetic and control group. It was more prominent in the pericyte basement membrane of diabetic rats (diabetic rats 112.5%, control 62.5%). Comparing the age related thickening of the pericyte basement membrane and endothelial basement membrane, the increase of the thickness of the pericyte basement membrane at 7 month-aged diabetic rats was statistically significant(p<.0005). Some degenerative changes of the basement membrane itself and cytoplasmic degeneration of the endothelial cell and pericyte were visible, and those were more prominent in diabetic rats. Some capillary endothelial cell shows thinning in 7 month-aged diabetic rats.