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Objective To observe the effect of Kadsura coccinea on the expression of Bcl-2, Bax and proliferating cell nuclear antigen (PCNA) in liver tissue of experimental hepatic fibrosis rats, to further discuss the mechanism of anti-liver fibrosis. Methods Sixty SD rats were randomly divided into normal control group, model control group, colchicine group, low-dose Kadsura coccinea group (2.5 g·kg-1) and high-dose Kadsura coccinea group (5 g·kg-1) (n = 12) . All groups except the normal control group were treated with CCl4, rich fat and poor protein to establishexperimental hepatic fibrosis animal model. The second day after modeling, drug treatmentwas started, till the end of the sixth week. Pathological section of the rat' s liver was examined in order to observe its tissue under a optical microscope. Liver tissues were taken to examine the degree of liver fibrosis by HE and Masson staining, and the expression of Bcl-2, Bax and PCNA protein were detected by immunohistochemistry. Results Kadsura coccinea relieved the degree of necrosis of liver cells, liver fat' s degeneration and collagen fiber hyperplasia significantly. Compared with the model control group, expression of Bax in low-,high-dose Kadsura coccinea group and colchicine group were significantly decreased, and the expression of PCNA in hepatic fibrosis rats were enhanced(P<0.05 or P<0.01) . Conclusion Kadsura coccinea has a certain inhibitory effects on experimental hepatic fibrosis in rats, and its mechanism may be related to inhibiting the expression of Bax protein and promoting the expression of PCNA protein in liver tissues.
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ObjectiveTo explore the effects of bone morrow stromal cells (BMSCs) on the neurological behavior of rats with traumatic brain injury (TBI).MethodsTwenty-four SD rats were randomly and equally divided into control group, TBI group and BMSC group. The weight-drop device was adapted to establish the TBI model. The injury severity and its outcome were evaluated by a set of criteria termed neurological severity score (NSS). Brain tissues were harvested at day 14 to observe the survival and migration of the transplanted cells.Bax expression was detected by RT-PCR. Results NSS was (12 ±3 ) points in the TBI group, significantly higher than (7 ± 1 ) points in the BMSC group (P ＜0.05). The transplanted BMSCs could survive and migrate. Moreover, BAX, a crucail apopotosis gene, was down-regulated to 0.9 ±0.1 in the BMSC group, compared with 1.1 ±0.2 in the TBI group (P ＜0.05). ConclusionsBMSC transplantation is available to improve the neurological function, as may be associated with the Bax.
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Objective To study the protective role of nerve growth factor (NGF) on cerebral ischemic reperfusion injury and its effect on Bax expression. Methods The models of focal cerebral ischemic reperfusion in rats were made by occlusion middle cerebral artery using an intraluminal filament method. NGF was given through vena caudalis. The expression of Bax was studied by immunohistochemistry method and the change of brain was observed using pathological method.Results Bax positive cells in the cortex and striatum areas in ischemia-reperfusion group (115.25?15.07) were more than those in NGF treatment group (70.00?12.77) ((P