Establishment of Quality Standard for Zhike Qutan Oral liquid / 中国药师

Objective: To establish the quality standard for Zhike Qutan oral liquid.Methods: A TLC method was used for the qualitative identification of Platycodonis Radix and Glycyrrhizae Radix et Rhizoma;the content of belamcandin in Belamcandae Rhizoma was determined by an HPLC method on a Waters Symmetry C 18 column(150 mm× 4.6 mm , 3.5 μm)with the mobile phase consisting of acetonitrile-water (14∶86), the flow rate was 1.0 ml·min-1 , the column temperature was 35℃ and the detection wavelength was 265 nm.Results: The TLC spots were clear without interference from the negative control.The linear range of belamcandin was 0.115-2.880 μg (r=0.999 9),and the average recovery was 92.44% (RSD=1.83% , n =6).Conclusion: The method is simple and rapid with good reproducibility, which can be used for the quality control of Zhike Qutan oral liquid.

Study on the Quality Standard of Belamcandin Standard Substance / 中国药房

OBJECTIVE:To establish the quality standard of Belamcandin standard substance.METHODS:Different indices of standard Belamcandin were examined with quantitative and quantitative analyses according to the corresponding measures stipulated in the Appendix of pharmacopoeia of people's republic of China(part Ⅱ)published in 2005.RESULTS:The specific rotatory power of 3 batches of standard Belamcandin was —28?～—30?([?]25 D,c0.11,Pyridine);the E1% 1 cmwas 790～830;the melting point was 252～254 ℃ and the ash content was less than 0.5%.The content of Belamcandin in the products was above 99.5% as revealed by HPLC.CONCLUSION:All of the indices were shown to meet the standards for standard substances and the established standard can be used for the quality control of standard Belamcandin.

HPLC Fingerprint of Rhizoma Belamcandae / 中国药房

OBJECTIVE: To establish the HPLC fingerprint of Rhizoma Belamcandae. METHODS: The fingerprint of Rhizoma Belamcandae was established by HPLC on Lichrospher C18(250 mm?4.6mm,5um).The mobile phase consisted of 1% acetic acid acetonitrile -1% acetic acid solution at a flow rate of 1.0 mL?min-1. The column temperature was 30 ℃.Cluster analysis was conducted with peak area as quantitative characteristic value. Canonical discriminant function and Fisher's discriminant function of the HPLC finger print of Rhizoma Belamcanda were established using stepwise classification scheme. RESULTS: The fingerprint of Rhizoma Belamcandae has been established. The discrimination and classification results by the two kinds of functions were totally consistent with each other, with corresponding rate of 100%. CONCLUSION: The two discriminant functions are of theoretical and practical value for they provide an accurate and rapid tool for the identification of the unknown samples as well as a new model for the quality control of Chinese medicines.