RESUMO
Objective To study the trend of antimicrobial resistance to cnical isolates of Klebsiella pneumoniae and Escherichia coli.Methods VITEK-32 automated analysis system was used for bacteria differentiation and drug resistance determination.Results TS From 2005 to 2008,the prevalence of ESBLs in isolates of K.pneumoniae and E.coil were increasing,their were 36.6% ,40.6% ,41.5% ,41.0% each year separately.The ESBLs-producing bacteria,multiple-drug-resistant to the third generation cephalesporin,aminoglycosides,quinolones and sulfonamides were also increased.Conclusion There is an increasing trend of ESBLs produces and resistences in K.pneumoniae and E.coli,It is important for clinical physicians to understand the distribution and the resistance characteristics of ESBLs producing K.pneumoniae and E.coli were to antibiotics,and to control the ESBLs spread.
RESUMO
Objective To investigate the genotypes of β-lactamases produced by Klebsiella pneumoniae.Methods Plasmid conjugation,PCR amplification,gene cloning and DNA sequencing,isoelectric focusing electrophoresis and extended-spectrum β-lactamase(ESBLs)confirmatory test were carried out for analyzing the encoding gene of β-lactamases in clinical strains of Klebsiella pneumoniae collected from hospital wards.Results Totally 75 clinical strains of Klebsiella pneumoniae were collected,in which 48 strains were confirmed to produce genotype of β-laetamases(64.0%),including 39 ESBLs-producing Btraim(52.0%).Among 48 strains,17 isolates(35.4%)carried 2 types of ESBLs genes,7(14.6%)carried 3 types of ESBL8 genes,and 5(10.4%)carried 4 types of ESBLs genes.CTX-M was the most comon type(30/48,62.5%),followed by TEM(26/48,54.2%)and SHV(25/48,52.1%).Among 9 isolates with DHA-1 AmpC β-laetamase,8 produced AmpC β-lactamases and ESBLs.Class A carbapenemase KPC-2 was produced in 3 isolates.False negative rate of ESBLs confirmatory test was 23.1%(9/39).Condusion Genotypes of β-lactamases produced by Klebsiella pneumoniae are complicated,which results in multi-drug resistance in clinic.
RESUMO
Objective Try to know the prevalence rate of ESBL-s produced by Eseherichia coil in our hospital from 2003 to 2007.Methods Antibiotic susceptibility were determined by K-B disc diffusion.Double-disk synergy tests performed on Mueller-Hinton agar plates was used for ESBL confirmation.Results There was 23.8% of our isolates can produce ESBLs in 2003,25.4% in 2004,31.3% in 2005,36.6% in 2006 and 44.9% in 2007,respectively.The situation of antibiotic resistance is more severe.Conclusion The antibiotic resistance should be well surveyed and the ESBLs must be routinely assayed.