Evaluation of Fibrinolytic Efficacy of Averrhoa Bilimbi Linn. by Using Euglobulin Lysis Time Method

Background: An impaired fibrinolytic function had been reported in subjects suffering from venous thrombosis. The euglobulin clot lysis time is a test that reflects the overall fibrinolytic activity of plasma. Objective: To evaluate the fibrinolytic efficacy of ethanolic extract of Averrhoa bilimbi Linn. leaves by using Euglobulin lysis time method. Methods: Rats were divided into 3 groups. Each group was containing 6 rats. Group I: received vehicle (control) 0.9% saline solution; Group II: received 25 mg/kg ethanolic extract leaves of Averrhoa bilimbi Linn; Group III: received 50 mg/kg ethanolic extract leaves of Averrhoa bilimbi Linn. Blood (1.8 ml) was removed with a plastic syringe containing 0.2 ml 3.8% sodium citrate solution. The time interval between the addition of thrombin and the complete lysis of the clots was measured. The lysis time (min) was determined. Conclusion: We observed ethanolic extract of Averrhoa bilimbi Linn. leaves may increase the activators of the fibrinolysis. Further investigation is warranted.

Anatomy, histochemistry and oxalic acid content of the leaflets of Averrhoa bilimbi and Averrhoa carambola

Abstract Averrhoa bilimbi L. and A. carambola L., Oxalidaceae, are the only two species of the genus Averrhoa L. Their leaves are widely used in folk medicine as an adjuvant in the treatment of diabetes. Some species may contain, for example, calcium oxalate crystals, which may lead to risk in its use when there is predisposition of individuals with reduced renal activity. Therefore, there are still few studies on the content of oxalic acid present in them, highlighting the importance of this investigation. The objective of this work was to conduct a comparative anatomical and histochemical study between the species and determining its content of oxalic acid. Semipermanent histological slides were prepared, following common plant anatomy procedures, for analysis of the leaflets in optical microscopy, polarization and scanning electron microscopy coupled with energy-dispersive X-ray spectrometry. To determine the total, soluble and insoluble oxalate content was used titration with potassium permanganate. The anatomical characterization allowed identifying the characters useful in the differentiation of the species. The histochemistry revealed the location of the metabolites. Chemical microanalyses demonstrated that the crystals are of calcium oxalate. A. carambola presented the highest levels of total oxalate and soluble oxalate. The study assists in the identification and quality control of A. bilimbi and A. carambola and brings new data on its oxalic acid content, which are important, in view of the medicinal use of the species.

Morfologia de frutos, sementes e plântulas de Averrhoa bilimbi L. oriundas de dois estágios de maturação / Fruits, seeds and seedlings morphology of Averrhoa bilimbi L. derived from two stages of maturation

Este trabalho teve por objetivo descrever as características morfológicas de frutos, sementes e plântulas de Averrhoa bilimbi L. oriundas de frutos imaturos e maduros. Os frutos de A. bilimbi foram separados de acordo com a sua coloração em frutos imaturos e maduros. O registro das características morfológicas dos frutos, sementes e plântulas foram realizados por meio de ilustrações manuais, fotografias e descrições de todos os aspectos morfológicos. Para o estudo da morfologia da germinação e plântula, foram semeadas quatro repetições de 25 sementes sobre substrato papel mata-borrão e levadas ao germinador tipo B.O.D., regulado à temperatura constante de 30°C e regime de luz contínua. Os frutos tanto imaturos como maduros variaram apenas quanto ao tamanho, consistência e coloração, sendo as demais características comuns à espécie. As sementes dos frutos imaturos e maduros diferenciaram-se quanto à turgescência dos tecidos, assim como sua coloração, brilho e textura. A germinação é do tipo epígea e fanerocotiledonar, com início no sétimo dia e término no décimo quinto dia após semeadura, com a obtenção da plântula normal. .

This study aimed to describe the fruits, seeds and seedlings morphological characteristics of Averrhoa bilimbi L. immature and mature fruits. The A. bilimbi fruits were separated according to color in immature and mature fruits. The record of fruits, seeds and seedlings morphological characteristics were performed by means of manual illustrations, photographs and morphological aspects descriptions. To study the germination and seedling morphology were take four replications of 25 seeds sown on blotter paper substrate and brought to B.O.D. adjusted in constant temperature 30°C under continuous light. The immature and mature fruits varied only in size, consistency and color, and other characteristics were common to the species. The seeds of immature and mature fruits could distinguished regarding the tissues turgor, as well as its color, luster and texture. The germination is phanerocotylar and epigeal, commencing on the seventh day and ending on the fifteenth day after seeding by obtaining normal seedling.

Effect of Traditional Sun-Drying on Phenolic Antioxidants of Averrhoa Bilimbi L.

Averrhoa bilimbi L. has various beneficial properties including antidiabetic and antioxidant activity. The effect of traditional sun drying on the stability of fresh A. bilimbi fruits was investigated by using different methanol/water extracts, and their total phenolic content (TPC) and total antioxidant capacity (TAC) compared. The TAC was evaluated using established in vitro models such as 1,1,diphenyl-2-picryl hydrazyl radical scavenging activity, 2,2´-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) scavenging assay, total reducing power, phosphomolybdenum assay and metal chelating activity. All the extracts of the dried fruit showed lower TPC compared to the fresh bilimbi extracts by 23-88%, TAC of which corresponded accordingly. The investigation revealed that A. bilimbi was a good source of antioxidants; however, the drying process of the fruit significantly affected the bioactive compounds.

Phytochemical screenings, thrombolytic activity and antimicrobial properties of the bark extracts of Averrhoa bilimbi.

In this present study, the bark extracts of Averrhoa bilimbi were subjected to the thrombolytic activities were assessed by using human erythrocyte and the results were compared with standard streptokinase (SK). On the other hand, bark extracts of A. bilimbi revealed moderate antibacterial activity against some microorganisms used in the screening. Preliminary phytochemical investigation suggested the presence of flavonoids, saponins and alkaloids.