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Distribution of 〔14C〕 bimolane in mice was studied by means of whole body autoradiography. At 30min, and lh, 3h, 9 h, 24h after iv, the mice were briefly anesthetized with ether and frozen by immersing indry ice/acetone. The mice were processed for whole body auto-radiography by continual sections with LKB-2250 PMV big whole body automatic-manual frozen cutter. The exeriments showed that 〔24C〕 bimolane distributed rapidly into the various tissues after iv administrtion. The highest radioactivity was found in lung, kidney, gastrointestinal tract, then the tumor, skin etc, the lowest radioactivity was found in brain.
RESUMO
AIM To compare the effects of probimane( Pro), bimolane ( Bim) and razoxane( Raz) on animal tumor metastasis in vivo . METHODS A biological inoculation method for assessment of pulmonary metastasis of Lewis lung carcinoma(3LL) was employed. RESULTS Pro and Bim inhibited the pulmonary metastasis of 3LL both from d 2 and from d 8 injections, but Raz only inhibited the pulmonary metastasis of 3LL from d 2 injections. Pro inhibited the pulmonary metastasis of 3LL more potently than Bim did at equitoxic dosage. CONCLUSION Pro is better in the treatment of pulmonary metastasis of 3LL than Raz for its possible novel molecular mechanisms.
RESUMO
It has been reported that bimolane can inhibit either humoral or cell-mediated immunological responses in vivo in mice & the inhibition of humoral immunity (by bimolane) is stronger than that of cellular immunity. Author investigated the influence of bimolane on the mitogen-induced mouse lymphocyte proliferation. Bimolane inhibited the lymphocyte proliferation induced by Con A(concana-valin A), PHA ( phy tohemagglutinin ) or LPS(lipopoiysaccharide) . Bimolane dose-dependently inhibited LPS-induced lymphocyte proliferation with remarkable inhibition at 1.56 mg/L bimolane. The inhibition of Con A-induced or PHA-induced lymphocyte proliferation by bimolane, however, began to appear at above 6.25 mg/L bimolane. The bimolane-mediated inhibition of the proliferation of the lymphocytes was not due to a nonspecific toxicity, because the preincubation of spleen cells with 25 mg/L bimolane for 24h did not make the cells died tested by trypanblau exclusion method.The data suggest that B lymphocytes are more sensitive to the bimolane-mediated inhibition than T lymphocytes.