RESUMO
RESUMEN: Las perforaciones radiculares son uno de los accidentes que pueden ocurrir dentro del tratamiento endodóntico rehabilitador de un diente y comprometen el pronóstico de éste. El objetivo de este reporte es describir el tratamiento y seguimiento de un diente con pronóstico desfavorable, con falsa vía y perforación en el 1/3 medio radicular. El manejo de la perforación se realizó con BiodentineTM y la rehabilitación con prótesis fija unitaria con poste de fibra individualizado. A los controles clínicos y radiográficos de 6, 12 y 24 meses el tratamiento se encontró asintomático y en función, pudiéndose considerar exitoso.
ABSTRACT: A radicular perforation is a mishap that can occur during the Endodontic and Restorative Treatment of a tooth and it can compromise its short and long-term prognosis. The aim of this report is to describe the treatment and follow up of a tooth with an unfavorable prognosis that had a perforation in the radicular middle third. The perforation was repaired with BiodentineTM and a fiber post and crown were placed. The patient had a follow up at 6, 12 and 24 months and the tooth was found asymptomatic and functional, which can be considered a clinical success.
Assuntos
Humanos , Feminino , Pessoa de Meia-Idade , Prognóstico , Terapêutica , Dente , Cavidade Pulpar , EndodontiaRESUMO
Biodentine™ is relatively a new tricalcium silicate cement that has gained great attention of the researchers due to its biological potential in comparison with other materials. The aim of this study was to investigate the optimum concentrations of Biodentine in relation to its stimulatory or inhibitory effect on proliferation, migration and adhesion of stem cells of human exfoliated deciduous teeth (SHED). The cell cultures of SHED were treated with Biodentine™ extract at four different concentrations; 20mg/ml, 2mg/ml, 0.2mg/ml and 0.02mg/ml. Cells cultured without Biodentine™ were kept as a blank control. The proliferation potential of SHED cells was evaluated by MTT viability analysis for 6 days. Migration potential was investigated by wound healing and transwell migration assays. The growth, survival and communication potential of these cells was determined by Adhesion assay. Results: A significant increase was observed in the proliferation and migration of SHED at (2mg/ml, 0.2mg/ml and 0.02mg/ml) while higher concentration of Biodentine™ (20mg/ml) exhibited cytotoxic effect on the cells. However, three tested Biodentine™ concentrations were similar in effect (non-significant) to adhesion ability of cells when compared with blank control. Conclusion: Our findings suggest that lower concentrations of Biodentine™ can be considered as the optimum concentrations to enhance the stimulatory effect of Biodentine on SHED.