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Objective To explore the bioeffect of different parameters on 4 cell lines by ultrasoundmediated microbubble destruction.Methods The orthogonal experimental design was used to investigate the effect of three factors on the bioeffects of four cell lines under three levels.Three factors included microbubble concentration,sound intensity,irradiation time.Human breast tumor (MCF-7) cells,ovarian tumor (A2780) cells,liver tumor (Bel7402) cells and thyroid tumor (ARO) cells were exposed to ultrasound in the presence of SonoVue.The cell survival rate was determined by MTT methods and the cell luminosity factor was detected by flow cytometry.Results The optimum parameters for Bel7402 and ARO cell were the same (A2B3C2),and they were different from those from MCF-7 (A3B1C1) and A2780 (A1B3C3) cell.The cell survival rates for 4 cell lines were above 75％,and the cell luminosity factors were different among 4 cell lines.Conclusions The optimum parameters by ultrasound-mediated microbubble destruction for different cell lines are different,and under the optimum parameters the bioeffects of different cell lines are different.
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The high quality and stability of Chinese materia medica (CMM) are the key factors to ensure clinical curative effect, and the ultimate goal of quality control (QC) for CMM is to ensure clinical efficacy. It is of significance how to establish a method for QC which could be related to clinical efficacy. According to the current situation and major problems of the QC for CMM, we first propose the efficacy-equivalent (EE) of CMM, and the research approach about EE has been elucidated, hoping to provide the reference for the establishment of the method of QC for CMM.
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Objective To explore the bioeffects of canine myocardium under microbubble destruction via diversity of ultrasonic intension, in order to optimize ultrasonic intension for experiments. Methods Nine mongrel dogs were randomly divided into 3 groups. Ultrasound (1 MHz) in diversity of different intension (0.5 W/cm~2, 1.0 W/cm~2, 2.0 W/cm~2) was applied to expose canine myocardium after intravenous injection microbubbles of 2.0 ml. All the dogs were killed after being exposed for 5 min. The myocardium was harvested for HE staining and observed with transmission electron microscope for the tissue microstructures. Results The myocardium of hyperemia, disfiguration and necrosis wer observed in all groups. Myocardial edema but not hemorrhage appeared with 0.5 W/cm~2 , mild myocardial hemorrhage and slight inflammatory cell infiltration happened with 1.0 W/cm~2, whereas obvious hemorrhage and certain degree of inflammatory cell infiltration occurred with intension of 2.0 W/cm~2. With the augmentation of ultrasonic intension, myocardium trend to aggravate. Conclusion Ultrasound of diversity intension can induce different bioeffects of canine myocardium. Ultrasound mediated microbubble destruction with the intensity of 1.0-2.0 W/cm~2 can provoke a certain degree of inflammatory reaction with mild myocardial damage.
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Ultrasound is the most important diagnostic modality in modern obstetrics. Theoretically, it may induce adverse effects in fetuses by either thermal or non-thermal effects. Hyperthermia is a recognized teratogen in animals. However, no confirmed bioeffects caused by exposure at intensities of present diagnostic ultrasound have ever been reported in human fetuses and the benefits of ultrasound overweigh the possible adverse bioeffects. The knowledge and skills of the users are a major determinant of the risk/benefit implications of the use of ultrasound. All users should understand the possible potential risks and receive specialized training in fetal ultrasound imaging. Recently, the use of new modalities such as Doppler and three-dimensional (3D) ultrasound has increased. There are few studies regarding the safety of 3D ultrasound despite rapidly increasing medical and commercial use. It is unacceptable to perform the ultrasound imaging of the fetus for non-medical purposes.