Expression and clinical significance of minichromosome maintenance protein 3(MCM3) in gastric cancer tissues / 国际生物医学工程杂志

Objective:To study the expression level and clinical significance of minichromosome maintenance protein 3(MCM3) in human gastric cancer.Methods:The expression levels of MCM3 mRNA and protein in gastric cancer and adjacent normal tissues were analyzed using public databases such as TCGA, CCLE and HPA. The clinicopathological features of 69 patients with gastric cancer were retrospectively analyzed, and the expression levels of MCM3 protein in tumor tissues and adjacent normal tissues were detected by immunohistochemical staining, and the relationship between it and clinicopathological features was analyzed. The interaction network of MCM3 proteins was analyzed using the STRING database.Results:The expression levels of MCM3 mRNA and protein in gastric cancer tissues were significantly higher than those in adjacent normal tissues ( P<0.05), and its high expression was correlated with the size of gastric cancer tumors ( P<0.05). In gastric cancer tissue, the expression of MCM3 was correlated with the expression level of proliferating cell nuclear antigen (PCNA) ( R=0.61, P<0.01), and there may be a protein-protein interaction. Conclusions:MCM3 plays an important role in gastric cancer by interacting with PCNA, and it is expected to become a new diagnostic and therapeutic target.

Bone marrow mesenchymal stem cells and tongue squamous carcinoma cells: Promoting growth or targeting biotherapy / 中国组织工程研究

BACKGROUND: Currently, the treatment methods for tongue squamous cell carcinoma mainly adopt comprehensive sequence therapy mainly based on surgery, but the therapeutic effect is still unsatisfactory. In recent years, tumor targeted biotherapy has attracted increasing attention. Stem cell technology enables therapeutic factors to be selectively delivered to tumor sites to play corresponding roles. Bone marrow mesenchymal stem cells show great advantages in this respect. However, the effect and action mechanism of bone marrow mesenchymal stem cells on tongue squamous cell carcinoma are still unclear. OBJECTIVE: To review the research progress on the effect of bone marrow mesenchymal stem cells on tongue squamous carcinoma cells, and clarify its function and discuss its mechanism. METHODS: The computer was used to search literatures about bone marrow mesenchymal stem cells in PubMed database, Wanfang database and CNKI database. The key words were “bone marrow mesenchymal stem cells, tumor cells”, “bone marrow mesenchymal stem cells, tongue squamous cell carcinoma”, “bone marrow mesenchymal stem cells, proliferation”, “bone marrow mesenchymal stem cells, migration, invasion” in Chinese, and “bone marrow mesenchymal stem cells, cancer cells” and “bone marrow mesenchymal stem cells, tongue squamous cell carcinoma”, “bone marrow mesenchymal stem cells, proliferation”, “bone marrow mesenchymal stem cells, migration, invasion” in English. A total of 43 articles were included for analysis. RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells have strong proliferation characteristics and can migrate directionally and promote invasion of tongue squamous carcinoma cells, suggesting that even if bone marrow mesenchymal stem cells have the function of promoting defect repair, and can also express therapeutic factors by gene modification and migrate to tumor growth site to play a therapeutic role, they cannot be used for repair treatment of postoperative defects of tongue squamous cell carcinoma. Targeted biological therapy of mesenchymal stem cells is a brand-new and promising field, but its safety still needs to be taken into account, and its mechanism of action and potential risks still need our continuous exploration.

Dosimetric evaluation of radiotherapy planning with internal biological target volume of non-small cell lung cancer based on PET-CT and 4DCT / 中华放射医学与防护杂志

Objective To compare the size of the internal target volume (ITV),biological target volume (BTV) and internal biological target volume (IBTV) based on PET-CT and 4DCT for primary nonsmall cell lung cancer (NSCLC),as well as try to apply IBTV in radiotherapy planning.Methods A total of 15 patients with NSCLC were sequentially scanned by an axial enhanced 3DCT,4DCT and 18F-FDG PET-CT in the thoracic region.The gross target volumes (GTVs) of ten phases of 4DCT images were contoured,and ITV was obtained by fusion of ten GTVs.BTV based on PET-CT images was determined by the SUV 2.0.The IBTV was defined by fusion of ITV and BTV.Planning target volumes (PTVs) based on ITV,BTV,and IBTV (PITV,PBTV,PIBTV) were obtained by ITV,BTV and IBTV with a 10-mm expansion respectively.The metrics of PIBTV,PITV and PBTV were compared,and the planning parameters of target volumes and risk organs were evaluated.Results There was no significant difference between ITV and BTV,but there was significant difference between IBTV and ITV and BTV (F=22.533,P ＜ 0.05).To include more than 95％ volume of IBTV,it is necessary to expand the margin of 9.0(6.0,12.0)mm based on BTV or 10.00(7.0,12.0)mm based on ITV.There was no significant difference between the two groups (P ＞ 0.05).Dice's similarity coefficient of BTV and ITV was 0.72(0.54,0.79).The intensity modulated radiotherapy plan based on PBTV can guarantee 85.6％ (80.5％,91.2％) of PITV to reach the prescription dose,compared with 80.2％ (74.4％,87.6％) of PBTV by the plan from PITV.Additionally,the conformity index and homogeneity index were not ideal.The dosimetric parameters of PITV and PBTV in the PIBTV plan were much better than those in PBTV-and PITV plan.Conclusions The radiotherapy plan based on PET-CT or 4DCT could not guarantee a reasonable dose distribution of PTV expanded from ITV or BTV.Thus,using IBTV for radiotherapy is advised.

Analysis of somatic mutations in membrane proteins of tumor cells / 中国生化药物杂志

Objective To screen specific mutations on extracellular regions of membrane proteins ( extracellular membrane protein mutations ) in tumor cells and provide the reference information for target searching in cancer precision medicine .Methods Somatic mutations on extracellular regions of membrane proteins of 7042 tumor samples were collected to screen all specific extracellular membrane protein mutations, and the overall distribution of these mutations were obtained by statistical analysis.Genes, gene site and cancer types occured high frequency of extracellular membrane protein mutations were identified.Results 97193 specific extracellular membrane protein mutations were obtained from 4938362 somatic mutations in 7042 tumor samples (30 cancer types), the statistical analysis showed that 4347 genes and 65532 sites were involved in these specific mutations.The study further analyzed five genes (MUC16、LRP1B、CSMD3、RYR2、USH2A), one site (17:37868208) and six cancer types (including colorectal cancer, melanoma, uterine cancer, brain lower grade glioma, lung adenocarcinoma and stomach adenocarcinoma) which occured high frequency of extracellular membrane protein mutations.Conclusion An information library of specific mutations on extracellular regions of membrane proteins was established and the distribution of these specific mutations was obtained which can provide reference information for target detection in targeted cancer therapy and immunological therapy.

Comprehensive measurement and analysis of multi-parameters from armored biological target under pistol impact / 医用生物力学

Objective To study the injury cases of armored biological target by bullet and its causes, and provide references for revealing the wound mechanism of armored human by bullet and the corresponding medical treatment. Methods A 60 kg live pig was selected as the biological target, and the testing physical quantity and specific location within the biological target were identified by reference to the vulnerability in the head and chest of the soldier with armor. Three rounds of 9 mm Bala Baerum pistols in 25 meter-range were shot, respectively, on the head and chest of the biological live target with armor, and the multi-mechanical parameters (acceleration, pressure, loads, etc.) that played an important role in blunt trauma of armored biological target under pistol impacts were measured. Results (1) Blunt injury to the head of the biological target by pistol generated negative pressure pulse inside the calvarium with far reaching effects, and pressure pulse appeared in the spine and carotid; (2) Blunt injury to the chest of the biological target by pistol caused high-G impact on the heart, with high pressure wave in the lungs. Conclusions The measurement results in this study provided the basis for quantitatively understanding the injury mechanism of the pistol impacted by live armored target.

Functional imaging and biological target volume:advances of experimental and clinical research / 中国癌症杂志

With the development of image technology,functional imaging was gradually applied to contouring the gross target volume,which generated the concept of biological target volume and biological intensity-modulated radiation therapy.In this review,we introduced our work and the current status of ~(18)F-fluorodeoxyglucose imaging,amino acids imaging,nucleic acid imaging,hypoxic imaging,gene imaging and molecular imaging.The future of functional imaging and it application in biological intensity-modulated radiation therapy were discussed in prospect.

Opportunity of interventional radiology:advantages and application of interventional technique in biological target therapy / 介入放射学杂志

Interventional techniques not only provide opportunity of treatment for many diseases,but also alter the traditional therapeutic pattern.With the new century of wide application of biological therapies, interventional technique also shows extensive roles.The current biological therapy,including gene therapy, cell transplantation therapy,immunobiologic molecule therapy containing cell factors,tumor antibody or vaccine,recombined proteins,radioactive-particles and targeting materials therapy,can be locally administrated by interventional techniques.The combination of targeting biological therapies and high-targeted interventional technique holds advantages of minimal invasion,accurate delivery,vigorous local effect,and less systemic adverse reactions.Authors believe that the biological therapy may arise a great opportunity for interventional radiology,therefore interventional colleagues should grasp firmly and promptly for the development and extension in this field.(J Intervent Radiol,2007,16:73-74)