Evaluation of toxin-induced injury and protective effect of triptolide based on a cardiovascular chip model / 药学实践与服务

Objective To construct a cardiovascular chip model for evaluating the damage of vascular glycocalyx induced by four marine toxins: okadaic acid (OA), conotoxin (CTX), tetrodotoxin (TTX) and gymnodimine (GYM), and explore the protective effect of triptolide on toxin-induced injury. Methods Human umbilical vein endothelial cells（HUVEC) were inoculated into a three-channel microfluidic chip. CCK-8 method and immunofluorescence staining were used to analyze the damage of cell viability and glycocalyx tissue induced by low, middle and high concentrations of marine toxin, as well as the protective effect of triptolide on toxin-induced injury. Results The cells in the cardiovascular chip grew well and had structurally intact glycocalyx. Compared with the control group, the activity of HUVEC cells were inhibited in group of the medium and high concentration of OA and high concentration of GYM (P<0.05). The activity of cells had not been inhibited by CTX and TTX significantly , but all the four toxins caused serious damage to the glycocalyx tissue (P<0.01). After pre-protection with triptolide, the toxicity of the four toxins to HUVEC cells and the damage rate of glycocalyx decreased significantly. Conclusion The four marine biotoxins could damage the activity and glycocalyx of HUVEC cells in a dose-dependent manner, while triptolide has a protective effect on HUVEC cells injured by toxin.

Aptamers specific for biotoxins: research advances / 国际药学研究杂志

Biotoxins widely exist in foods and environments, posing a serious threat to human health. Specific and sensitive detection of biotoxins is the most effective way to prevent unexpected problems caused by biotoxins. In recent years, aptamer-based bio-toxin detections have gradually been prevailing. Aptamers, a new type of recognition molecules with high affinity and specificity, have been used to produce a variety of biosensors. In addition, some aptamers can neutralize the toxicity of biotoxins and inhibit their activities, with the effect to replace the antibody to some extent. This article briefly describes the research advances on biotoxin aptamers and prospects the development and application of aptamers targeting biotoxins in the future.

Prevention and treatment of biotoxin-induced acute renal injury / 解放军医学杂志

With the increase of human activities, biotoxin poisoning has gradually become a serious health problem. Biotoxins are usually classified into bacterial toxins, mycotoxins, marine toxins, animal venoms, enterotoxins, exotoxins, etc. A variety of toxins can cause impaired renal function and even lead to acute renal failure. The biotoxin-induced kidney injury is a dangerous clinical condition involving a variety of pathophysiological mechanisms. Failure to treat properly in time often leads to higher mortality. Blood purification therapy plays a central role in the treatment of patients with biotoxin-induced renal injury. The present paper reviews the current status of prevention and treatment of acute renal injury caused by common biotoxins in China, including poisonous mushrooms, fish gall, snake venom and bee stings. The purpose of this review is to make Chinese nephrologists and specialists clear the prevention and treatment of biotoxin-induced renal injury, and standardize the prevention and treatment strategy of biotoxin-induced kidney injury.

Aptamer-based technologies in the detection of marine biotoxins / 国际药学研究杂志

Aptamers are kinds of short single-stranded deoxyribonucleic acid(ssDNA)or ribonucleic acid(RNA)selected in vitro using SELEX(systematic evolution of ligands by exponential enrichment). They can effectively bind various targets such as ami-no acids,carbohydrates,proteins,cells and viruses,with the advantages of simple operation,high sensitivity and low cost. This arti-cle outlines the basic principles,advantages and applications of aptamer technology. It also summarizes the features and hazards of ma-rine biotoxins,and introduces the application prospects of aptamer technology in the detection of marine biotoxins.

Recent technique development in marine biotoxin analysis and detection / 第二军医大学学报

Widespread distribution, strong toxicity, and difficulty to detoxicatemakemarine biotoxins a great threat to the nation' public security and health. To meet this threat, great endeavor has been devoted to marine biotoxin analysis. Up to now the methods for marine biotoxins analysis include bioassay test, immunoassay, liquid chromatography, enzyme activity assay, cytotoxicity test, etc. In this review, we summarized the major technique developments in marine biotoxins analysis.

Applications of snake venoms in treatment of cancer

Snake venoms are folk medicines used since ages. The components of snake venoms have high specific affinity and actions on cells and cell components. Also snake venoms are largely cytotoxic to tumor cells than normal cells. In addition to these, they have several therapeutic actions that make them an attractive option in the management of cancer. The advent of modern technologies has greatly helped in extracting and identifying new components of therapeutic interests in short time. The article highlights the importance of snake venoms in the management of cancer, so as to motivate curious researchers to devote their skills in this fascinating area. This in turn may bring hope, smile and relief to several cancer patients in future.

Construction,expression and in vitro specific killing activity of a recombinant fusion protein:anti-CD20Fab-LDM / 中国药理学通报

Aim To construct and express anti-CD20Fab-LDP,generate anti-CD20Fab-LDM and identify its biological activity.Methods PCR and overlapping PCR were used to construct anti-CD20Fab-LDP.DNA sequence was analyzed by the Terminus of Dideoxy Nucleotide.The product was purified by affinity chromatography and analyzed by Western blot and its antigen-binding activity was examined by FACS.Specific killing activity in vitro of anti-CD20Fab-LDM was analyzed by MTT.Results The data of DNA sequence showed that anti-CD20Fab-LDP was correct.The fusion protein was recovered in high yield(up to 4 mg·L~(-1))after proteinG purification.The fusion protein could bind to Raji cells(CD20+),and similar affinity data were obtained with anti-CD20Fab.Anti-CD20Fab-LDP showed potent cytotoxicity to Raji cells with IC_(50) values of 0.9×10~(-10) mol·L~(-1).Conclusions Anti-CD20Fab-LDP with high level expression was successfully obtained and could bind to Raji cells cells.Anti-CD20Fab-LDM showed specific killing activity to Raji cells in vitro.