Niaogan Prescription protects bladder epithelial cells against uropathogenic Escherichia coli invasion / 中草药

Objective To observe the role of Niaogan Prescription against uropathogenic Escheriehia coli (UPEC) in invading bladder epithelial cells, and to discuss its mechanism of action. Methods After HTB-9 infected with UPEC, the cell invasion model was used to observe the protective effects of drug urine in human bladder cancer cell 5637 (HTB-9) against UPEC invasion. Effects of Niaogan Prescription on the main steps of signaling pathway: Toll-like receptor 4 (TLR4), adenylate cyclase 3 (AC3), cyclic adenosine monophosphate (cAMP), protein kinase A (PKA), and Rac-1 was investigated by molecular biology techniques. Results Compared with the blank urine high dose group (10% volume fraction of blank urine), treatment with the drug-containing urine high dose group of Niaogan Prescription (10% volume fraction of drug urine) resulted in a significant decrease in UPEC invasion, the bacterial invasion rate of the rat blank urine group had no significant difference compared with the model group. Compared with the blank urine high dose group, the drug-containing urine high dose group of Niaogan Prescription improved TLR4 and AC3 protein expression, increased intracellular cAMP content, promoted PKA activation, and inhibited Rac1 activity. Conclusion Niaogan Prescription have the certain capacity against UPEC invasion of bladder epithelial cells, which mechanism is related to TLR4/cAMP signaling pathway. Niaogan Prescription; uropathogenic Escheriehia coli; invasion; human bladder epithelial cells; TLR4/cAMP signal pathway

Chitosan promotes proliferation of rabbit bladder epithelial cells in vitro / 第二军医大学学报

Objective: To study the influence of chitosan on proliferation of bladder epithelial cells, so as to discuss its feasibility in treatment of interstitial cystitis. Methods: Bladder epithelial cells were harvested by enzymatic digestion of the epithelium of New-Zealand rabbit bladder. The cells were cultured in different concentrations of chitosan(0.3, 0.6, 1.2, 2.4 and 4.8 g/L) for 72 h; untreated cells served as control. The growth and proliferation of cells were observed under microscope. The effects of chitosan on proliferation of cells were studied by NAG assay and cell counting. Results: Immunohistochemistry staining revealed that the cultured cells were epithelial cells. Chitosan (>0.3 g/L) promoted the growth of epithelial cells, and the promoting effect was significantly when the concentration of chitosan was 1.2 g/L (P<0.01). The promoting effects were decreased when the concentrations of chitosan were 2.4 and 4.8 g/L, but were still higher than that of the control group (P<0.01). Conclusion: Chitosan can promote the growth of the bladder epithelial cell in vitro, which might contribute to the treatment of interstitial cystitis.

Experimental study of chitosan on proliferation of bladder epithelial cells in vitro in rabbit ZHENG / 中华泌尿外科杂志

Objective To study chitosan's improving proliferation effect to the bladder epithelial cells,thus providing experlimental foundation for the treatment of interstitial cystitis.Methods Bladder epithelial cells were harested by the enzymatic digestion of the epithelium exposed by the eversion of reseeted New-Zealand hare's bladder.The cells were cultured in different concentrations(0.3、0.6、1.2、2.4、4.8 g/L)of chitosan group and control group,after 72 h,observing their growth and proliferation with optical microscopy;The effects of chitosan on proliferation of rabbit bladder epithelial cells were studied by NAG assay.EGFR mRNA was measured by PT-PCR.Results The growth of cells in the sample added chitosan is much better than that of in the control group.Chitosan could promote the proliferation of bladder epithelial cells at higher than 0.3 g/L of concentration in a dose dependent way.The optimum concentration to increase proliferation of eonjunctival epithelial cells was 1.2 g/L.The proliferative effect of EGFR mRNA increased with the elevated chitosan concentration after 72 h.Conclusions Chitosan can promote the growth of the bladder epithelial eell,which can provides a valuable evidence for further studies of interstitial cystitis's treatment.This proliferation effect is perhaps related to chitosan's promoting EGFR combinating specificity with EGFR.

Chitosan promotes proliferation of rabbit bladder epithelial cells in vitro / 第二军医大学学报

0.3 g/L)promoted the growth of epithelial cells,and the promoting effect was significantly when the concentration of chitosan was 1.2 g/L(P