Metabonomic study of blood of mice with high-voltage electrical injury / 局解手术学杂志

Objective To explore the changes of metabonomics in blood of mice after high-voltage electric shock,then screen out the significantly changed differential metabolites and metabolic pathways.Methods The head of C57BL/6J mice was subjected to high-voltage electric shock(electric shock group)or exposed to acoustic and optical stimulation of high-voltage electric(control group),then the whole blood from mice were collected to separate serum.The dual platform combined metabonomic analysis based on gas chromatography-mass spectrometer(GC-MS)and liquid chromatography-mass spectrometer(LC-MS)was performed and orthogonal partial least squares discriminant analysis(OPLS-DA)was used to screen the differential metabolites and related metabolic pathways.Results A total of 415 differential metabolites were screened out in metabonomics in blood of mice after high-voltage electric shock,including 187 up-regulated and 228 down-regulated metabolites.These differentially metabolites were significantly enriched in metabolic pathways including central carbon metabolism in cancer,glucagon signaling pathway,etc.Conclusion By establishing the model of high-voltage electrical injury on experimental mice,this study reveals the significant change of metabolite content and metabolic pathway in blood by high-voltage electrical injury.Which provides a basis for the damage of blood metabolic activity by high-voltage electrical injury,and suggests the potential harm of high-voltage electrical injury to blood metabolic activity in the whole body.

Relationships between solar greenhouse working and selected blood biochemical indexes of elderly workers / 环境与职业医学

Background The aging of the labor force has become a global trend, and various occupational activities can affect blood biochemical indexes and increase the risk of metabolic diseases. Elderly workers are the main laborers in the solar greenhouse industry, and their blood biochemical indexes need more attention. Objective The purpose of this study is to explore the alternation of selected blood biochemical indexes and provide clues for conducting management programs of metabolic diseases in the elderly solar greenhouse workers. Methods The research subjects of the greenhouse group and the non-greenhouse group were selected from a vegetable production base in Northwest China. The elderly workers were selected from a recruited subjects pool for an earlier project by means of matching and non-matching respectively. The matching conditions were gender (consistent), age (±2 years old), body mass index (BMI) (±1 kg·m−2), education level (±1 level), smoking (consistent), and drinking (consistent). Routine clinical tests were used to detect serum total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDLC), low-density lipoprotein cholesterol (LDLC), fasting blood glucose (GLU), and albumin (ALB), and single-factor and multi-factor analyse were used to identify different indexes in matching and non-matching samples, respectively. The matching and the non-matching samples included 80 pairs of subjects and 327 subjects, respectively. Results In the matching sample set, the greenhouse group's HDLC [(1.61±0.34) mmol·L−1] was lower than the non-greenhouse group's [(2.09±0.37) mmol·L−1] (P<0.05), the level of TG/HDLC in the greenhouse group was (1.17±0.74), higher than that in the non-greenhouse group (0.78±0.47) (P<0.05), and the level of LDLC/HDLC in the greenhouse group was (1.87±0.36), also higher than that in the non-greenhouse group(P<0.05), while there was no statistical difference of the other indexes between the two groups (all Ps>0.05). In the non-matching sample set, the results of multiple linear regression analysis showed that solar greenhouse working correlated with decreased HDLC, and increased TG/HDLC and LDLC/HDLC among the elderly workers (all Ps<0.05). Conclusion Solar greenhouse working may correlate with variations of selected blood biochemical indexes in elderly workers, and the occupational health surveillance of elderly workers should be strengthened.

Analysis of electrocardiogram and blood related indicators in workers occupationally exposed to mercury in Jiangsu Province from 2016 to 2018 / 中华劳动卫生职业病杂志

Objective@#To investigate the health status of occupational mercury workers and reveal the effects of mercury exposure on the cardiovascular system.@*Methods@#In June 2019, a total of 2651 mercury workers participated in the occupational health examination between 2016-2018 from a thermometer manufacturing plant and a fluorescent lamp manufacturing plant were included in this study. Then, they were divided into a high-level mercury exposure group (425 workers whose urine mercury concentration >35 μg/g creatinine) and a low-mercury mercury exposure group (2226 workers whose urinary mercury concentration <35 μg/g creatinine) . Mercury concentration in the workplace was also detected. Finally, the results of electrocardiogram (ECG) , blood routine, blood biochemistry and other physical examinations were analyzed. The measurement data of age and exposure years were analyzed by test. Urinary mercury and blood parameters were analyzed by Mann-Whitney nonparametric test. Chi-square test was used for the analyses of gender, ECG abnormality rate and other categorical data.@*Results@#The 8-hour weighted average allowable concentration (CTWA) of mercury in the workplace of high-exposure group was 0.002 2-0.152 mg/m3. The abnormal rate of ECG in the high-exposed group (29.6%) was higher than that in the low-exposure group (10.1%) in 2018 (P<0.01) . Compared with the low-exposure group, the WBC of the high-exposure group from 2016 to 2018 was increased, with statistically significance (P<0.05) ; the RBC of the high-exposure group in 2016 and 2017 was decreased, with statistically significance (P<0.01) ; the total bilirubin concentration in the high-exposure group was decreased from 2016 to 2018, with statistically significance (P<0.05) .@*Conclusion@#Long-term exposure to high concentration of mercury in the workplace may influence cardiovascular system. Therefore, engineering protection and individual protection should be implemented well.

Study on Long-term Toxicity of Liqi Sanjie Extractum in Rats with Intragastric Administration / 中国药房

OBJECTIVE： To study the long-term toxicity of Liqi sanjie extractum in rats after intragastric administration， and to provide reference for safety evaluation before clinical practice. METHODS： A total of 160 rats were randomly divided into control group （normal saline） and Liqi sanjie extractum low-dose， medium-dose and high-dose groups （7.828 0， 15.656 0， 31.312 0 g/kg， calculated by crude drug）， with 40 rats in each group. They were given relevant medicine intragastrically once a day from Monday to Saturday. The experimental period was 120 days， and the recovery period was 30 days after the end of the experiment. General information of rats was observed， and body weight and feed consumption of rats were measured once a week. At the 61st day of administration， the end of administration and the end of recovery period， 10， 20 and 10 rats were collected from each group to observe their hematology， blood biochemistry， organ coefficient and histopathology changes. RESULTS： From 61st day to 120th day of administration， the rats of Liqi sanjie extractum high-dose group had hair loss and erection， and recovered after withdrawal of medicine. During medication， the body weight of mice in Liqi sanjie extractum low-dose and medium-dose groups increased faster than control group， while the body weight of rats in Liqi sanjie extractum high-dose group increased slower than control group. Compared with control group， the feed consumption of Liqi sanjie extractum low-dose group increased， while those of Liqi sanjie extractum medium-dose and high-dose groups decreased； the rats were recovered after drug withdrawal. On the 61st day of administration and after the end of administration， some hematological indexes， blood biochemical indexes and organ coefficients of rats in administration group were significantly different from those of control group （P＜0.05 or P＜0.01）. The hematology， blood biochemistry and organ coefficients of rats were basically recovered after the end of the recovery period. The number of erythrocyte， hematocrit， standard deviation of erythrocyte width， albumin， globulin ratio and potassium K+ levels in Liqi sanjie extractum low-dose group were significantly lower than those in control group （P＜0.05 or P＜0.01）. The absolute value of intermediate cells in blood of rats in Liqi sanjie extractum medium-dose group was significantly higher than that of control group （P＜0.05）， and the mean hemoglobin concentration， K+ and uterine coefficient in blood were significantly lower than those of control group （P＜0.05）. The number of white blood cells， absolute value of lymphocyte， absolute value of intermediate cells， the percentage of intermediate cells， prothrombin time and spleen coefficient in Liqi sanjie extractum high-dose group were significantly higher than those in the control group （P＜0.05 or P＜0.01）. Mean hemoglobin concentration， granulocyte percentage， albumin， alkaline phosphatase and K+ were significantly lower than those in the control group （P＜0.05 or P＜0.01）. No abnormalities in systemic autopsy and histopathology were noticed in rats. CONCLUSIONS： Long-term intragastric administration of Liqi sanjie extractum can cause certain toxic reactions in rats， and low dose of Liqi sanjie extractum causes less and lighter toxic reactions which can be automatically recovered after drug withdrawal. It can provide reference for the determination of clinical safe dose.

Study on correlation of fundus arteriosclerosis patients and their blood biochemical indexes / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM: To investigate the blood biochemical indexes in total cholesterol, low density lipoprotein cholesterol and blood glucose levels and the correlation of eyegrounds arteriosclerosis.<p>METHODS: This study selected 2017-02/2018-01 97 cases eyegrounds arteriosclerosis patients of our hospital blood biochemical indicator detection as observation group. The other selected line during the same period to our health physical examination results of 98 cases of normal subjects as control group. Detection of two groups of blood biochemical indicators, and then the comparison.<p>RESULTS: The total cholesterol, low density lipoprotein cholesterol, blood glucose and blood pressure levels in observation group were significantly higher than control group(<i>P</i><0.05). When patients blood biochemical routine blood glucose, total cholesterol and low density lipoprotein was high, the degree of eyegrounds arteriosclerosis was higher. By incorporating multiple factors analysis, blood glucose, blood pressure, total cholesterol and low density lipoprotein levels and eyegrounds arteriosclerosis were positively correlated.<p>CONCLUSION: The blood glucose, total cholesterol, blood pressure, and low density lipoprotein level was higher, the eyegrounds arteriosclerosis in patients was more serious, eyegrounds arteriosclerosis level by clinical testing for treatment monitoring, diagnosis, pathological changes.

Analysis of hematological and biochemical parameters of heterozygous SGT-deficient mice in young and elderly ages / 中国比较医学杂志

Objective To study the effect of heterozygous deficiency of SGT gene on the hematological and biochemical parameters of mice in young and elderly ages.Methods Blood samples were analyzed for complete hematological and biochemical parameters from heterozygous SGT-deficient and wild-type mice of 10-weeks and 6-months old mice, respectively.Results Age-related changes in most indexes were found statistically significantly different between young and elderly mice with the same genotype.Compared with the wild type at the same age, the platelet large cell ratio (P-LCR) was lower in young heterozygous SGT-deficient mice.However, platelet count, plateletcrit (PCT) and neutrophil count were more significantly lower in elderly heterozygous SGT-deficient mice (P<0.05).There was no significant difference for biochemical parameters ALT, AST, LDH, urea nitrogen, creatinine and blood glucose.Total and unconjugated bilirubin as well as ALP were significantly higher in elderly heterozygous SGT-deficient mice but not for conjugated bilirubin (P<0.05).In addition, significant differences existed for the lipids between two elderly groups (P<0.05).Conclusions Heterozygous deficiency of SGT gene induced changes of some hematological and biochemical parameters in elderly mice.It provides helpful information for further investigation on SGT involvement in some biological and pathological processes.

Biological characteristics of Chinese hamster infected with Babesia / 中国比较医学杂志

Objective To establish a Chinese hamster model of babesia infection, to find the changing pattern of organs and biochemical parameters in Chinese hamster infected with Babesia, and to promote the detection and treatment of babesiosis.Methods Healthy 5-week old Chinese hamsters were infected by intraperitoneal injection of blood containing Babesia.Blood samples were collected at 0, 2, 4, 6, 8, 10, 12, 14, 16, 23, 30, and 37 days after infection from 5 hamsters at each time point.Blood smears were prepared to detect the parasites using Giemsa staining.ELISA assay was employed to test the IL-2 concentration.The blood biochemical indexes were detected using an automatic biochemical analyzer.DNA was extracted from the whole blood and REAL-TIME RCR was performed to determine the reproduction of Babesia.Aftert the animals were sacrificed, the heart, lung, spleen, liver, and kidney were taken to analyze the changes of organ coefficients.Results The highest level of Babesia in the hamsters occurred on day 4 after the Babesia injection, and then showing a decreasing tendency.However, there was a transient increase on the 12th day after infection.The liver and spleen displayed most extensive response to the infection showing hepatomegaly and splenomegaly, but the variation of heart and kidneys coefficients was within the norm.There were prominent changes of blood cells, especially leucocytes, with two peaks at day 10 and 23 after the Babesia infection.The peak changes of blood biochemical indexes occurred at day 12 after infection.The concentration of serum IL-2 reached a peak on the 10th day after infection.Conclusions The Chinese hamsters display typical characteristics of tick-borne diseases such as hepatomegaly and splenomegaly.The immunological system is activated along with the infection and reaches a highest stage in the second week.Afterwards the Babesia can live in the hamster body for a long period of time.The results of this study provide useful information supporting further studies on the detection, treatment and prevention of Babesiosis.

Comparative study on values of blood physiological and biochemical for middle and old-aged cynomolgus monkey / 中国比较医学杂志

Objective To provide reference of the general physiological index, blood physiology and biochemistry for middle and old-aged cynomolgus monkey.Methods 119 cynomolgus monkey aged from 11~16 (80 were males and 39 were females) were involved in the study.We detected the general physiological index( body weight, the crown-rump length and waist circumference ) , hematology and blood biochemistry parameters respectively and compared these parameters between males and females.Results Between males and females, there were significant differences in Body weight, the crown-rump length and Waist circumference of the general physiological index(P 0.05).Conclusion It provides reference of general physiological index, blood physiology and biochemistry parameters of cynomolgus monkey and basis for their application in biomedical domain.

Determinação das concentrações plasmáticas de proteínas e metabólitos de cascavéis em cativeiro / Determination of proteins and metabolites concentrations in plasma of rattlesnakes in captivity

Para determinar as concentrações plasmáticas de proteínas e metabólitos de cascavéis em cativeiro, foram utilizadas 60 serpentes adultas, sendo 30 machos e 30 fêmeas. O sangue foi coletado através de punção do seio venoso paravertebral cervical e armazenado em tubos com heparina. As análises bioquímicas foram processadas colorimetricamente em Analisador Automático de Bioquímica Chemwell (Awareness Technology®, Inc). Foram calculadas as médias e desvios padrão dos seguintes constituintes: proteínas totais, albumina, globulinas, relação albumina/globulinas, ácido úrico, creatinina, ureia, colesterol, colesterol HDL e triglicérides. Os valores obtidos foram semelhantes aos descritos na literatura para repteis e serpentes, sendo as diferenças observadas provavelmente decorrentes da diferença entre espécies, clima, estação do ano e metodologia utilizada. Não houve diferenças significativas entre machos e fêmeas para os parâmetros estudados. Estes resultados podem ser úteis no estabelecimento de valores de referência para planos de conservação destes ofídios em cativeiro.(AU)

For determining plasma concentrations of proteins and metabolites of rattlesnakes in captivity, 60 adult snakes, 30 males and 30 females were used. Blood was collected by puncture of the cervical paravertebral venous sinus and stored in tubes with heparin. Biochemical analyzes were colorimetrically processed using an Automatic Biochemistry Analyzer Chemwell (Awareness Technology®, Inc). The mean and standard deviation were calculated for the following constituents: total protein, albumin, globulin, albumin/globulin ratio, uric acid, creatinine, urea, cholesterol, HDL cholesterol and triglycerides. The values were similar to those previously reported for reptiles and snakes, with the differences observed probably due to the difference between species, climate, season and the methodology used. There were no significant differences between males and females for the parameters studied. These results may be useful in establishing normal biochemical values for conservation plans for these snakes in captivity.(AU)

ESTUDIO POBLACIONAL DE LA TORTUGA CAREY Eretmochelys imbricata (CHELONIIDAE)EN EL PACÍFICO SUR DE COLOMBIA / Population Study of the Hawksbill Turtle Eretmochelys imbricata (Cheloniidae) in the Southern Pacific region of Colombia

El objetivo de esta investigación fue conocer aspectos poblacionales, biológicos y ecológicos de las tortugas carey (Eretmochelys imbricata) presentes en el Pacífico sur de Colombia (Departamento del Cauca), para esto se tomó información de morfometría, se revisó el estado de salud de los individuos y se tomaron muestras de sangre para valoración de química sanguínea. Durante siete meses de muestreo se capturaron 25 tortugas carey (16 individuos diferentes) en los arrecifes del Parque Nacional Natural Gorgona, con un porcentaje de recaptura del 46 %. Aunque no se notaron problemas evidentes de salud, la mayoría de animales presentaron gran parte del caparazón cubierto de algas filamentosas y algunos epibiontes sobre el plastrón y el caparazón. La Longitud Curva Caparazón (LCC) mostró una mayor proporción de individuos con tallas entre 37 y 45 cm de longitud (prom. 42,3 cm; min. 37,5 cm; max. 58 cm). Por medio de la prueba de Mann Whitney U, se comparó la LCC de los 16 individuos diferentes capturados en el PNN Gorgona, con 11 individuos capturados en la zona del litoral del Departamento del Cauca, encontrándose diferencias significativas entre estos dos grupos, donde los animales presentes en el PNN Gorgona fueron de mayor talla que los presentes en el continente (Z = -2,59; p = 0,007). Los valores de ácido úrico se encontraron por encima de los valores de referencia.

The objective of this research was to determine biological and ecological population characteristics of the hawksbill turtle (Eretmochelys imbricata) found in the southern Colombian Pacific department of Cauca. Morphometric measurements were recorded, the health status of individuals was reviewed, and blood samples were taken for a biochemistry assessment. During the seven months of the investigation, 25 hawksbill turtles were caught (16 different individuals) on the reefs of Gorgona Natural National Park. Forty-six percent of the total numbers of turtles assessed were recaptured during the study period. While no obvious health problems were noted, most animals possessed epibionts and filamentous algae covering the carapace, some parts of the limbs, as well as on their neck. Curved carapace length (CCL) showed the highest proportion of individuals were between 37 and 45 cm. Sixteen individuals captured in Gorgona Natural National Park were compared with 11 individuals captured in the coastal zone of the department of Cauca. Using the Mann-Whitney U test, a significant difference in CCL was found between these two groups; the animals from Gorgona National Park were larger than those present on the coast of the mainland (Z = -2.59, p = 0.007). Uric acid concentrations were found to be higher than previously referenced values.

Valoración de los efectos fisiológicos y neuroquímicos de altas concentraciones de tetrahidrocannabinol en modelos biológicos / Assessment physiological and neurochemical effects of high concentrations of tetrahydrocannabinol in biological models

El objetivo de esta investigación fue evaluar los efectos fisiológicos y neuroquímicos en 60 ratones machos cepas Naval Medical Research Institute (NMRI) en edad adulto-joven con pesos promedios de 25,45 ± 3,05 g, sometidos durante seis semanas a dosis del principio psicoactivo de la marihuana el Δ-9-tetrahidrocannabinol en concentraciones entre 4 - 20%. Se realizaron tomas de sangre retroorbital para evaluar parámetros hematológicos y bioquímicos antes, durante y post experiencia. Se monitorearon medidas tales como: peso, ingesta de agua, alimentos, actividad locomotora horizontal y vertical, entre otros. Al final de la experiencia se realizo autopsia y toma de muestras de regiones cerebrales, para medir niveles de neurotransmisores aminoacidicos y dopamina. Estos resultados permiten concluir que altas concentraciones del principio psicoactivo de la marihuana hacen más dependiente al consumidor con los consecuentes daños fisiológicos y neurológicos. Esto lleva a que cada vez se necesite más droga para producir el mismo efecto.

The objective of this research was to evaluate the physiological and neurochemical effects in 60 (Naval Medical Research Institute) NMRI male mice strains in young adult - age average weight 25.45 ± 3.05 g, underwent six weeks at doses of the psychoactive ingredient in marijuana the Δ -9-tetrahydrocannabinol in concentrations between 4-20 %. Retroorbital blood shots were conducted to evaluate hematological and biochemical parameters before, during and post experience. Weight, water intake, food, horizontal and vertical locomotors activity include: measures such as monitored. At the end of the experience autopsy was conducted and sampling of brain regions to measure levels of amino acid neurotransmitters and dopamine. These results suggest that high concentrations of the psychoactive ingredient in marijuana consumers become more dependent with consequent physiological and neurological damage. This leads to more and more drugs is needed to produce the same effect.

Biochemical values of red-tailed boa (Boa constrictor) / Valores bioquímicos de jiboia (Boa constrictor)

It was determined the average values of the following blood biochemical indicators of boas (Boa constrictor): glucose, urea, creatinine, aspartate aminotransferase (AST), alanine transaminase (ALT), amylase and lipase, and compared the results obtained concerning sex. A total of 12 Boa constrictor specimens were used, seven males and five females. The average of the biochemical indicators between males and females had no significant difference. The traditional biochemical techniques have been useful to determine these indicators to this species...

Foram determinados os valores médios dos indicadores bioquímicos sanguíneos: glicose, ureia, creatinina, aspartato aminotransferase (AST), alanina aminotransferase (ALT), amilase e lipase de jiboias (Boa constrictor) e comparados os valores das concentrações encontradas entre os grupos de machos e fêmeas. Foram utilizados 12 espécimes de Boa constrictor, dos quais sete machos e cinco fêmeas. A comparação das médias dos indicadores bioquímicos entre os grupos de machos e fêmeas indicou ausência de influência significativa de fatores sexuais. As técnicas bioquímicas tradicionais foram adequadas para a determinação desses indicadores para esta espécie...

Evaluation of acute toxicity of babassu mesocarp in mice

The safety of babassu mesocarp (Orbignya phalerata Mart., Arecaceae), which exhibited anti-inflammatory and antithrombotic activities, was evaluated by determining the potential acute toxicity in mice. A lyophilized ethanol extract of babassu mesocarp (BME) was administered to C3H/HePas mice (10/group) in a single dose of 1000, 3000 and 5000 mg/kg, by gavage. General behavior adverse effects and mortality were determined for up to fourteen days. Selected biochemical parameters including glucose, triacylglyceride, cholesterol, urea, alkaline phosphatase and creatinine were determined by colorimetric assay. The heart, liver, spleen, kidneys and brain were weighted and evaluated macro and microscopically. The median lethal dose (LD50) of BME was greater than 5000 mg/kg. No behavior or body weight alterations were detected after the treatment. The acute treatment with BME has no effect on macroscopic and microscopic aspect of examined organs. Instead, BME increased the alkaline phosphatase and reduced the urea concentration in all groups. A significant increase on triacylglyceride was detected in the group BME1000. In conclusion, the acute treatment with high doses of BME can affect some biochemical parameters with a long lasting effect, although any change was detected at tissue level or body and organ weight.

Relationship among blood indicators of lipomobilization and hepatic function during early lactation in high-yielding dairy cows

Blood indicators are used as a tool to diagnose metabolic disorders. The present work was conducted to study the relationships among blood indicators of lipomobilization and hepatic function in high-yielding dairy cows. Two groups of Holstein cows were studied: 27 early lactation cows and 14 mid lactation cows from four different herds with similar husbandry characteristics in Galicia, Spain. Blood samples were obtained to measure beta-hydroxybutyrate (BHB), non-esterified fatty acids (NEFA), triglycerides (TG), and the activity of aspartate transaminase (AST) and gamma-glutamyl transferase. Cows in early lactation had higher levels of BHB and NEFA than mid lactation cows. High lipomobilization (NEFA > 400 micromol/L) was detected in 67% and 7% of early lactation and mid lactation cows, respectively, while subclinical ketosis (BHB > 1.2 mmol/L) was detected in 41% and 28% of the early lactation and lactation cows, respectively. TG concentrations were low in all cows suffering subclinical ketosis and in 61% of the cows with high lipomobilization. During early lactation, 30% of cows suffered hepatic lipidosis as detected by levels of AST. Compromised hepatic function was observed in early lactation cows as shown by lower concentrations of glucose, total protein, and urea.

Bioquímica plasmática de cascavéis (Caudisona durissa LINNAEUS, 1758) em cativeiro / Blood biochemical parameters from captivity rattlesnake (Caudisona durissa LINNAEUS, 1758)

Acidentes com seres humanos envolvendo a espécie Caudisona durissa possuem a mais alta taxa de letalidade dentre os viperídeos brasileiros. Ressalta-se então a importância para a saúde pública da criação dessa espécie em cativeiro para produção de soro antiofídico. No entanto, essa atividade ainda hoje encontra alguns desafios como a instalação de doenças no plantel, evidenciando a importância de estudos sobre a fisiologia de serpentes. Dessa forma, foram realizadas análises de bioquímica plasmática em 53 serpentes da espécie Caudisona durissa, mantidas em cativeiro. Foram utilizadas amostras de plasma com heparina e as dosagens bioquímicas realizadas em aparelho automatizado (Ciba Corning - Express Plus®). Os resultados e seus respectivos desvios-padrões foram: uréia -1,32mg dL-1 (±1,1); ácido úrico - 2,08mg dL-1 (±1,4); creatinina - 0,52mg dL-1 (±0,2); proteína total - 3,7g dL-1 (±0,7); albumina - 1,62g dL-1 (±0,4); globulinas - 2,08g dL-1 (±0,5); cálcio - 15,25mg dL-1 (±2,8); fósforo - 4,61mg dL-1 (±1,9); colesterol - 171,58mg dL-1 (±52,7); triglicerídeos - 19,29mg dL-1 (±14,3); fosfatase alcalina - 31,04U L-1 (±12,4); aspartato aminotransferase (AST) - 22,25U L-1 (±11,4); alanina aminotransferase (ALT) - 7,11U L-1 (±5,4) e Amilase - 1385,23U L-1 (±568,7). Foram calculados os seguintes índices: relação uréia/creatinina - 2,5 e relação cálcio/fósforo - 3,3. O conjunto de resultados das análises bioquímicas do sangue das cascavéis em cativeiro pode servir como referência para apoio diagnóstico na espécie estudada e para outros trabalhos com o mesmo caráter de observação.

Human accidents involving rattlesnake Caudisona durissa have the highest fatality rate among the Brazilian Viperidae family. Breeding this specie in captivity in order to produce antivenoms is very important to public health. Nevertheless, there are some challenges that this activity must face, like the onset of diseases on the breeding stock, justifying the importance of studying these snakes' physiology. Blood biochemical analysis of 53 Caudisona durissa was performed using plasma samples in an automated equipment (Ciba Corning - Express Plus®). Mean values and its respective Standard deviations are: blood urea nitrogen (BUN) -1.32mg dL-1 (±1.1); uric acid - 2.08mg dL-1 (±1.4); creatinine - 0.52mg dL-1 (±0.2); total protein - 3.7g dL-1 (±0.7); albumin - 1.62g dL-1 (±0.4); globulins - 2.08g dL-1 (±0.5); calcium - 15.25mg dL-1 (±2.8); phosphorus - 4.61mg dL-1 (±1.9); cholesterol - 171.58mg dL-1 (±52.7); triglycerides - 19.29mg dL-1 (±14.3); alkaline phosfatase - 31.04U L-1 (±12.4); aspartate aminotransferase (AST) - 22.25U L-1 (±11.4); alanin aminotransferase (ALT) 7.11U L-1 (±5.4); amylases - 1385.23U L-1 (±568.7). The following indexes were calculated: BUN/creatinine ratio - 2.5 and calcium/phosphorus ratio -3.3. Rattlesnake blood biochemical results above could be used as reference in clinical evaluation for captivity C. durissa.

Efectos de la solución CM-95 tratada magnéticamente sobre parámetros hematológicos y bioquímicos en ratones Balb/c / Effects of the magnetically treated CM-95 solution on hematologic and biochemical parameters in Balb/c mice

Se evaluaron los efectos de la solución CM-95, tratada con campo magnético estático en el intervalo de 0,01-0,16 T e inoculada una vez en un volumen de 0,2 mL en ratones Balb/c por vía intraperitoneal y la misma solución sin tratamiento magnético en animales sin inocular, para valorar su acción sobre los parámetros hematológicos y bioquímicos del metabolismo celular de estos roedores desde la fase preclínica, con vista a lo cual se procesó la sangre obtenida por sangrado total después del esquema terapéutico, así como también se calculó el número total y diferencial de leucocitos a partir del líquido sanguíneo. En el equipo autoanalizador automático Hitachi 902 se analizó el plasma extraído de cada muestra de sangre y se determinaron, entre otros elementos: proteínas totales, glucosa, creatinina, transaminasas, iones de calcio y colesterol. Se evidenció que la solución CM-95 tratada magnéticamente produjo cambios en los leucocitos sanguíneos, dados por un aumento significativo de los linfocitos con respecto a los controles, así como también en los parámetros bioquímicos, entre los cuales se modificaron los valores de glucosa, transaminasas y colesterol, pero todos dentro de límites normales para la especie. Tales resultados abren nuevas perspectivas para el posible uso de este sistema acuoso, tratado con magnetismo, en biomedicina

The effects of the CM-95 solution, treated with static magnetic field in the interval of 0,01-0,16 T and inoculated once in a volume of 0,2 mL in Balb/c mice intraperitoneally and the same solution without magnetic treatment in animals without inoculation were evaluated, in order to value its action on the hematolologic and biochemical parameters of the cellular metabolism of these rodents from the preclinical phase. For this purpose, the blood obtained through total bleeding was processed after the therapeutic pattern, and the total and differential number of leukocytes was calculated using the blood. The extracted plasm of each sample of blood was examined in the automatic autoanalyzer equipment Hitachi 902 and among other elements, total proteins, glucose, creatinine, transaminases, calcium ions and cholesterol were determined. It was evidenced that the magnetically treated CM-95 solution produced changes in the blood leukocytes, given by a significant increase of the lymphocytes compared to the controls, as well as in the biochemical parameters, among which the values of glucose, transaminases and cholesterol were modified , but all within the normal limits for the species. Such results provide new perspectives for the possible use of this watery system, treated with magnetism, in biomedicina

Effect of Rosiglitazone on oxygen free radical and biochemical parameters in aged SD rats / 第二军医大学学报

Objective: To observe the effect of Rosiglitazone (RGTZ) on oxygen free radical and biochemical parameters of aged rats, so as to establish a basis for anti-aging study. Methods: The 24-month-old naturally aged SD rats were randomly divided into the control group (CONT), the RGTZ group and the caloric restriction group(CR). The RGTZ group was given intragastric administration of RGTZ (4 mg · kg-1 · d-1) and the CR group was given 60% of the diet that of the CONT group. The rats were killed 12 weeks later and the serum malondialdehyde(MDA) content, superoxide dismutase(SOD) activity and the blood biochemistry parameters were examined. Results: The blood glucose (GLU), aspartate transaminase (AST), alamine transaminase(ALT), serum creatinine (SCr), and blood urea nitrogen (BUN) were within the normal range in all groups. Compared with the CONT group, blood cholesterol (CH) in RGTZ group was lower, but with no significant difference. The serum triglyceride(TG) of both RGTZ and CONT groups decreased markedly than those in the CONT group(P<0.05). The activities of SOD and MDA were improved in RGTZ and CR group compared with the control group. Conclusion: RGTZ can regulate the lipid metabolism and attenuate the oxidative damage in aged rats, indicating its anti-aging effect.

Níveis iônicos e enzimáticos de cutias (Dasyprocta sp.) hígidas, criadas em cativeiro, influência do sexo e da idade / Ion and enzymatic levels of healthy agouti (Dasyprocta sp.) raised in captivit. Influence of gender and age

Determinou-se o perfil do ionograma e enzimas hepáticas de cutias (Dasyprocta sp.) saudáveis, criadas em cativeiro, como também se avaliou a influência de sexo, idade e interação sexo-idade. Foi adotado um delineamento inteiramente ao acaso, em arranjo fatorial 2 x 4 (dois sexos e quatro faixas etárias), com três repetições, totalizando 24 cutias. Foram determinados os valores para o cálcio (Ca), fósforo (P), cloretos (Cl), aspartato aminotransferase (AST), alanina aminotransferase (ALT), fosfatase alcalina (FA) e gama-glutamiltransferase (GGT). Os valores médios obtidos foram: Ca= 7,62+2,59mg/dl; P= 3,91+1,41mg/dl; Cl= 58,63+16,45mg/dl; AST= 119,54+79,35UI/ml; ALT= 28,08+15,53UI/ml; FA= 26,95+14,01UI/ml e GGT= 25,34+19,44UI/ml. O valor de P foi maior nas fêmeas e da FA nos machos. Os níveis de FA diminuíram com o aumento da idade.

This research studied the profile of the ionogram and hepatic enzymes of healthy agoutis (Dasyprocta sp.) raised in captivity as well as evaluated the influence of gender, age and interaction gender-age. It was used a completely randomized design, in a factorial arrangement of 2 x 4 (two genders and four age groups), with three repetitions, totaling 24 agoutis. The values were determined for calcium (Ca), phosphorus (P), chlorides (Cl), aspartate aminotransaminase (AST), alanine aminotransaminase (ALT), alkaline phosphatase (ALP), and gamma-glutamyltransferase (GGT). The mean values were: Ca= 7.62±2.59mg/dl; P= 3.91±1.41mg/dl; Cl= 58.63±16,45mg/dl; AST= 119.54±79.35UI/ml; ALT= 28.08±15.53UI/ml; ALP= 26.95±14.01UI/ml, and GGT= 25.34±19.44UI/ml. The value of P was larger in females and ALP in males. As the age increased, levels of ALP decreased.

Experimental Study of Tiaozhi Baogan Capsule in Preventing and Treating Fatty Liver in Rats / 中药新药与临床药理

Objective To investigate the preventive and therapeutic effects of Tiaozhi Baogan Capsule (TBC) on fatty liver in rats and to explore its therapeutic mechanism. Methods SD rat models of fatty liver were set up by feeding high fat diet. After the treatment of TBC, the general physical state, liver index (the ratio of liver weight with body weight) , hepatic pathological changes , blood biochemical indices were observed in model rats. Results TBC could obviously ameliorate liver pathological changes in model rats, relieve hepatic steatosis and hepatocellular necrosis, decrease the content of malondialdehyde( MDA) and increase the vitality of superoxide dismustase (SOD) in serum and liver, lower the content of total cholesterol (TC) and low- density lipoprotein (LDL) in serum and decreae the liver index (P

THE DIETARY FEATURES AND BLOOD BIOCHEMISTRY IN OBESE CHILDREN / 营养学报

Objective: To study the dietary habits and serum leptin levels of obese children and its possible association to lipids and glucose metabolism, serum lipids and C peptide (CP) in children with different body weight. Methods: 52 obese children were inquired about their favorite foods and food intake. 145 healthy children (85 boys, 60 girls), aged 6-13, with a wide range of body mass index (BMI) 14 to 30 kg/m 2, were measured for fasting serum leptin, CP, glucose, lipids (TG, TC, LDL C,HDL C), height and weight. The children were classified into three groups by BMI, i.e. normal weight group (A group), overweight and slight obese group (B group). moderate and severe obese group (C group). Results: 65% of obese children liked meat best. 38.46% of them ate most in supper. The serum leptin, CP, TG in obese children were higher than that in normal children. Leptin concentration of girls were higher than that in boys in A and B groups (P