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Objective To compare the hepatic differentiation potential of human umbilical cord mesenchymal stem cells (UC-MSCs) with bone marrow mesenchymal stem cells (BM-MSCs).Methods UC-MSCs and BM-MSCs derived from passage 3 were induced by IMDM supplemented with 20 μg/L HGF and 20 mg/L α-FGF.The medium was changed twice a week.The concentrations of albumin and urea nitrogen from cultural medium were measured to compare the differentiation ability of the two cells.We also examined the expression of hepatic related genes by real-time RT-PCR.Results UC-MSCs manifested significandy stronger proliferation potential than BM-MSCs.Both UC-MSCs and BM-MSCs could be induced into hepatocyte-like cells.The morphology of UC-MSCs tended to be more mature than BM-MSCs and they had more cytoplasmic granules.After 4 weeks,the levels of albumin and urea nitrogen from the cultural medium of the UC-MSCs group were significantly higher than the BM-MSCs group (P ＜ 0.05).Real-time PCR showed the expressions of four liver related genes CK18,G6P,HGF and ALB in the UC-MSCs group were significantly higher than the BM-MSCs group (P ＜ 0.05).Conclusion UC-MSCs had higher hepatic differentiation potential than BM-MSCs.Thus,UC-MSCs are more suitable than BM-MSCs for tissue engineering in the treatment of end-stage liver diseases.
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Objective To investigate the feasibility of transplantation of mesenchymal stem cell (aisc) into expanion skin. Methods MSCs were isolated from porker's bone marrow and cultured in vitro. Pigs were randomly divided into four groups: Group A was injected with MSCs on the local expansion; Group B was injected with MSCs from ear vein; Group C was planted expander only; Group D was the contronl group. Each side of pig's spinal column was implanted with three expander in groups A, B and C. The same volume of NS was injected at the fixed time, the marked area measured after 7, 14, 28 days of expansion, the difference of those area was compared between groups. The differentiation of BM-MSC was detected by immunofluorescence. Results Flow-cytometric analysis showed that these BMSCs expressed CD90 and CD29 highly but did not express CD34 or CD45. The expansion area (cm2) of groups A, B, C and D was 34.05±0. 92, 31.83±l. 07,30. 10±0.79, and 18. 27±0.25, respectively (P＜0. 01). Immunofluorescence showed that the positive expression rate of CD31, PCNA in groups A and B was higher than that in groups C and D, in which the expression was the highest in group A. Conclusions Allogeneic transplantation of BM-MSC can promote skin expansion and the effect of local transplantation group is most significant.