Methods for determination of serum albumin in the assay of glycated albumin / 安徽医科大学学报

A total of 306 fresh human serum samples were randomly selected. Alb concentrations were measured by bromcresol green ( BCG) method, modified bromcresol purple ( mBCP) method, and immunoturbidmetic assay ( ITA) , respectively. GA was measured by an enzymatic method. GA value was expressed as the percentage of GA in the total serum Alb [ GA%(%) =GA/Alb?100%] . When Alb≥40 g/L, the clinical differences between BCG and mBCP, BCG and ITA, and mBCP and ITA were not significant, and there was no statistical difference between GA% BCG and GA% mBCP(P=0. 537); when Alb<40 g/L, BCG had statistical difference between mBCP and ITA ( P<0. 01 ) , and GA% BCG was significantly lower than GA% mBCP ( P<0. 01 ) . No obvious clinical signifi-cance of Alb concentrations was observed measured by BCG, mBCP, and ITA when Alb≥40 g/L. There was no difference in different Alb methods for GA% calculation. When Alb<40 g/L, the consistency of mBCP and ITA was better than that of BCG and ITA, which indicated that mBCP method may be more suitable for the assay of gly-cated albumin value ( GA%) , whereas the GA% may be underestimated if using BCG method for the determination of Alb.

Design of method comparison study and bias estimation for albumin assays / 中华检验医学杂志

Objective It describes procedures for designing an analyzing a method comparison experiment for serum albumin(ALB) assay and determining the relative bias between two methods using split patient samples.Method According to the procedure described by the NCCL approved guideline: method comparison and bias estimation using patient samples; 40 patient samples were analyzed in 5 operating days, analyze each patient sample in duplicate using both BCG and BCP method. The duplicates were assessed for each method within the same run. The coefficient of correlation was calculated as well as the bia between the methods.Results In the patient′s serum sample, the bias between BCG and BCP methods were 1.8% at 50g/L or 5.5% at 40 g/L or 11.6% at 30 g/L for albumin. In the commercial quality control serum products, the bias between the two methods varied up to 36%.Conclusion In determination of the patient′s ALB, the bias between BCG and BCP goes up when the concentration of ALB is decreased. But the bias between the two methods varies up to 36% when the quality control serum is determined.

The measurement of albumin in urine by the bromcresol green method compared with the results of albustix and robert’s test.

For determination of albumin in urine, Bromcresol Green (BCG) was used as follows: stock BCG regent was diluted to 1:20, and 2.5 ml. of the reagent was added to 100 µl of specimen; the resulting reaction mixture was measured for optical density at 630 nm. The values of the routine Rebert’s Test and reagent strip (Albustix) were compared to those produced by the BCG method. The mean albumin values of the BCG method were 22.4, 54.6, 95.6, 180.4, 396.3 and 1,176.3 mg/dl which were comparable to the negative, trace, 1+, 2+, 3+, 4+ values of the Robert’s Test, respectively. The mean albumin values of the BCG method were 26.3, 52.7, 96.3, 191.7, 476.1 and 1,273.1 mg/dl which were comparable to the negative, trace, 1+, 2+, 3+, 4+ values of the Albustix, respectively. The albumin values from Albusix and the Robert’s Test could be estimated from this study.