RESUMO
Objective To find changes of urinary biomarkers of hydroxyproline (HYP) and C-terminal telopeptide of collagen type Ⅱ (CTX-Ⅱ) after celecoxib and anti-osteogenic tablets treatment among adult Kaschin-Beck disease (KBD) patients in Qinghai Province,and provide the scientific data for treatment effect of adult KBD.Methods According to the "Diagnosis of Kaschin-Beck Disease" (WS/T 207-2010),120 cases KBD patients and 89 cases healthy control were divided into three groups,including drug treatment group,non-drug therapy group and control group.The drug treatment group was taken celecoxib (200 mg/pill,1 pill/day,1 time/day) and anti-osteogenic tablets (0.4 g/pill,4 pills/time,2 times/day) for 6 month.The urine samples of these persons were detected with the method of enzyme linked immunosorbent assay (ELISA) for analyzing HYP and CTX-Ⅱ.The results were corrected by creatinine (Cr).Results There were 54 cases of KBD patients (24 males,30 females) in drug treatment group with average age of (47.51 ± 12.30) years old;there were 66 cases of KBD patients (31 males,35 females) in non-drug therapy group with average age of (46.85 ± 13.57) years old;and there were 89 healthy people (41 males,48 females) in control group with average age of (48.75 ± 13.92) years old.By comparing the three groups,there were no statistical significant differences in gender (x2 =0.820,P ＞ 0.05) and ages (F =0.379,P ＞ 0.05).Medians of urinary HYP contents among drug treatment group,non-drug therapy group and control group were 62.47,106.04,65.80 μg/μmol·Cr,respectively,and the difference was statistically significant (Z =12.114,P ＜ 0.01);medians of urinary CTX-Ⅱ contents among drug treatment group,non-drug therapy group and control group were 555.23,702.92,495.54 ng/μ mol· Cr,respectively,and the difference was statistically significant (Z =20.454,P ＜ 0.01).Conclusion Levels of HYP and CTX-Ⅱ among adult KBD patients have changed after treatment with celecoxib and anti-osteogenic tablets,and can be used to determine the effect of treatment.
RESUMO
Objective To detect urinary bio-markers of hydroxyproline (HYP) and c-terminal telopeptide of collagen type Ⅱ (CTX-Ⅱ) among population from Kashin-Beck disease (KBD) regions in Qinghai Province,and to provide the scientific data for prevention and control of adult KBD.Methods According to the "Diagnosis of Kashin-Beck Disease" (WS/T 207-2010),using case-control study,120 KBD patients (males 55,females 65) and 89 healthy controls (males 41,females 48) in Qinghai KBD regions were divided into case group and control group.Morning urine samples were collected.HYP and CTX-Ⅱ contents were analyzed by enzyme-linked immunosorbent assay (ELISA),then these results were corrected with creatinine.All the data were analyzed by SPSS 17.0 software.Results There was no significant difference in the age of male and female between case group and control group (t =1.813,1.131,P ＞ 0.05).The medians of urinary HYP and CTX-Ⅱ contents among male patients were 74.91 μg/μmol Cr and 630.77 ng/μmol Cr,respectively,which were higher than those of control groups (51.38 μg/μ mol Cr,401.32 ng/μmol Cr,Z =3.068,3.246,P ＜ 0.01).The medians of urinary HYP and CTX-Ⅱ contents among female patients were 91.07 μg/μmol Cr and 637.17 ng/μmol Cr,respectively,compared with those of control groups (88.37μg/μmol Cr,546.47 ng/μmol Cr),there was no significant difference in HYP content (Z =0.273,P ＞ 0.05),however,the difference in CTX-Ⅱ content was statistically significant (Z =2.002,P ＜ 0.05).Conclusion The urinary HYP contents of male patients with KBD change significantly,while the degradation of type Ⅱ collagen in male and female patients increases,and CTX-Ⅱ could reflect the metabolic changes of collagen in KBD.