CD4+CD25+CD127low/- regulatory T cells and related cytokines in patients with psoriasis vulgaris / 实用医学杂志

Objective To explore the role of CD4+CD25+CD127low/-regulatory T cells (Tregs) in the pathogenesis of psoriasis vulgaris(PV). Methods Flow cytometry analysis was used to detect the amount of Tregs in peripheral blood and ELISA to test the levels of IL-10 and TGF-β1 in blood serum; the suppressive function of Tregs on autologous CD4+CD25-T cells was determined by MTT method. Results No significant difference was found in the proportion of Tregs in PV patients and healthy controls(P>0.05). There was a diminished suppression of Tregs from patients on autologous CD4+CD25- responder T cell proliferation in PV patients when compared with that in controls (P < 0.01). The serum level of IL-10 in patients was lower than that in controls (P < 0.01) while that of TGF-β1 in PV patients was significantly higher than that in controls(P < 0.01). Conclusion Abnormal function of Tregs and low secretion of IL-10 in PV patients might be related to the pathogenesis of psoriasis.

Changes of regulatory T cells in peripheral blood samples from patients with lung cancer and their correlation with clinicopathologic features of lung cancer / 中华微生物学和免疫学杂志

Objective To investigate the changes of CD 4+CD25+CD127 low regulatory T ( Treg ) cells in peripheral blood samples from patients with lung cancer and their correlation with clinicopathologic features of lung cancer .Methods Flow cytometry was used to measure the percentages of CD 4, CD8, nat-ural killer ( NK) and Treg cells in peripheral blood samples collected from 160 patients with lung cancer and 60 healthy subjects .The correlations between the levels of Treg cells and clinicopathologic features of lung cancer were analyzed .The percentages of Treg cells in 60 patients with lung cancer before and after surgery were compared .Results The percentages of CD 4+and NK cells and the ratios of CD 4+/CD8+cells in pa-tients with lung cancer were significantly lower than those in healthy control , while the percentages of Treg cells in patients with lung cancer were decreased as compared with those in healthy subjects .The percenta-ges of Treg cells in patients with advanced cancer were significantly higher than those in patients at early stage (P<0.05), which dropped significantly after surgery (P<0.01).Conclusion The results of this study indicated that Treg cells in patients with lung cancer might inhibit antitumor immune responses and correlate with the progression of cancer .It would be worthwhile to check Treg cells in patients with lung cancer for monitoring the prognosis and treatment effects .

Graves patients peripheral blood dendritic cells and CD4+CD25+CD127low/-Tr cell changes observation / 中华微生物学和免疫学杂志

Objective To observe the numerical characteristics of dendritic cells (DC),the DC subsets(myeloid dendritic cell,MDC; plasmacytoid dendritic cell,PDC) and CD4+ CD25 + CD127low/-Tr cells in peripheral blood of Graves disease (GD) patients.Methods According to the clinical manifestations and serum FT3,FT4 and TSH,the GD patients were divided into the untreated-group,the clinical remission group and the clinical stable group,and set normal control group as well.The flow cytometry was used to detect DC and CD4+CD25+CD127low/-Tr cells of the percentage of CD4+ T cells in subjects peripheral blood(EDTA-K2 anticoagulated).The indicators were compared among various groups,and the correlation between the indicators with serum FT3,FT4 and TSH were observed.Results (1) In the untreated-group,the clinical remission group,the clinical stable group,and normal control group,total DCs,MDCs and MDC/PDC gradually declined,untreated-group has a significant difference from the other three groups also the significant difference was found among other three groups; (2) In the untreated-group,the clinical remission group,the clinical stable group,and normal control group,PDCs declined successively,but only the difference was found between untreated-group and normal control group; (3)In the untreated-group,the clinical remission group,the clinical stable group,and normal control group,CD4+CD25+CD127low/-Tr cells gradually raised,but only the difference between untreated-group and normal control group make sense; (4)In the untreated-group,PDCs and CD4+CD25+CD127low/-Tr cells have a certain relevance; (5)There was good correlation between DCs and serum FT3,FT4 and TSH,but CD4+CD25+CD127low/-Tr cells only have correlation with FT3 and FT4.Conclusion DC,MDC,MDC/PDC increased in the untreated-GD patients,and decreased after the therapy of anti-thyroid.Therefore,DCs and the DC subsets are expected to be used to monitor GD in the course of disease.CD4+CD25+CD127low/-Tr cells can be used as a new indicator of the onset of GD.

Expression of galectin-10 in CD4~+ CD25~+ CD127~(low/-) regulatory T cells / 临床检验杂志

Objective To investigate the expression of galectin-10 in CD4+CD25+CD127low/-regulatory T cells(Treg) and the correlation between expressions of galectin-10 and Foxp3.Methods CD4+CD25+CD127low/-Treg cells and CD4+CD25-CD127+ T cells were isolated from PBMC of healthy volunteers and lymph nodes of gastric cancer patients by flow cytometry.Following RNA micro-extraction RT-PCR analysis was used to detect mRNA expression of galectin-10 and Foxp3.Results The mRNA expression of galectin-10 and Foxp3 were dominant in Treg cells,whereas nearly no expression in CD4+CD25-CD127+ T cells.The results from PBMC of healthy volunteers and lymph nodes of gastric cancer patients were consistent between.Conclusion The expression of galectin-10 was obviously high in Treg cells,so it may become a novel marker for phenotypic characterization of Treg cells.

The Expression and Clinical Significance of CD~4+CD25~+ Regulatory T cell in Non-Hodgkin's Lymphoma / 医学研究杂志

Objective To evaluate the expression of CD4+CD25+CD127low regulatory T cells(Tregs) in the peripheral blood of the patients with NHL and discuss the relationship between expression of Tregs and prognostics factors of NHL.Methods 58 NHL patients was enrolled for analyzing.Among of them,23 were new patients without any treatment,35 patients received 3 to 8 cycles of chemotherapy,and 16 were normal controls.The expression of CD4+CD25+CD127low Tregs was detected by Flow Cytometry and the proportions of Tregs were analyzed among the groups of the patients before or after chemotherapy and the normal controls.Bivariate Correlation was used to analyze the correlation among the expression of Tregs in the peripheral blood and prognostic factors,such as age,sex,PS state,LDH,?2-MG,IPI,clinical stage and pathology category of the new patients.Results The proportion of Tregs in the peripheral blood of B cell lymphoma(B-NHL) group(3.89?2.49)% was significantly higher than that of normal control(2.52%?0.88%)(P=0.005).In B-NHL,Tregs was signifantly higher in the aggressive NHL(DLBCL,MCL) than that of nonaggressive HHL(FL,MZL)(P=0.002).In T cell lymphoma,Tregs was increased markedly in the highly aggressive T-NHL(T-LBL,NK/TCL) as compared with aggressive T-NHL(ALCL,PTCL)(P=0.006).Tregs expression was significantly higher in newly diagnosed NHL patints than that of normal control(P=0.004) and post-chemotherapy group(P=0.034).The expression of Tregs in peripheral blood was significantly correlated with ?2-MG,LDH and IPI of the patients in newly diagnosed patients group(P=0.003,0.003 and 0.038),whereas was not significantly correlated with the age,sex,PS state,LDH,clinical stage and pathology category of the patients.Conclusion The expression of CD4+CD25+CD127low Tregs in peripheral blood of B-NHL group was significantly higher than that of the patiens post-chemotherapy and normal control,which means that it may be an useful prognostic factor for NHL patients.