Increased CD5 + B Cells in Neonatal Infections / 대한면역학회지

PURPOSE: CD5+ B (B1) cell is a subpopulation of B cells and CD5+ B cells constitute a large fraction of B cells in neonates. CD5 B cells are closely related with autoimmune diseases but the roles and functions in neonates are still unknown. The quantitative changes of CD5+ B cells in neonatal infections were examined to investigate the involvement of CDS+ B cells in neonatal immune reaction to general immunologic stimuli such as infections. Methods: Ten normal neonates and eight neonates with acute febrile diseases were studied. Venous blood was drawn and mononuclear cells were separated by Ficoll-Hypaque. Half was double-stained with FITC-conjugated anti-CD5 and PE-conjugated anti-CD19, and another half with FITC-conjugated anti-CD4 and PE-conjugated anti-CD8. Stained samples were analyzed using fluorescent-activated cell sortor. ...continue...

Decreased CD5+ B cells during the acute phase of Kawasaki disease

We investigated the changes of CD5+ B cells in the peripheral blood of 20 Kawasaki disease (KD) patients. The percentage of CD5+ B cells in the total lymphocytes and in the total B cells significantly decreased during the acute phase of KD(p< 0.01), compared to that in the age-matched normal control subjects. After intravenous immunoglobulin(IVIG) treatment, the percentage of CD5+ B cells increased, but was still lower than that in the normal controls(p< 0.01). During the convalescent phase of the disease, the percentage of CD5+ B cells was restored to the normal levels. The levels of CD5+ B cell percentage in the total B cells of the patients with acute febrile disease showed similar levels to age-matched normal controls. The decreased CD5+ B cells in the patients with KD provides an additional abnormal immunological finding during the acute phase of the disease.

Effects of intravenous immune globulin on the peripheral lymphocyte phenotypes in Kawasaki disease

The effect of intravenous immune globulin (IVIG) on the lymphocyte phenotypes in acute Kawasaki disease (KD) was studied in a random trial of IVIG-and-aspirin versus aspirin-alone. Before therapy, patients in each treatment group had an increased percentage of B cells, and a decreased percentage of T cells, CD4 T cells, CD8 T cells and CD5+ B cells. There was no significant difference in immunologic parameters between the two groups measured before therapy. Patients treated with IVIG-and-aspirin had by the fourth day developed a highly-significant increase in T cells, CD4 T cells and CD8 T cells and a decrease in B cells. Despite the decrease of B cells, there were significant increases in CD5+ B cells in both treatment groups. However, the degree of increase in the IVIG-and-aspirin treated group was significantly more noticeable than that in the aspirin-alone treated group. These findings indicate that treatment with IVIG restores the T- and B- cell abnormalities, especially CD5+ B-cell abnormalities found in patients with acute KD.

Effects of intravenous immune globulin on the peripheral lymphocyte phenotypes in Kawasaki disease

The effect of intravenous immune globulin (IVIG) on the lymphocyte phenotypes in acute Kawasaki disease (KD) was studied in a random trial of IVIG-and-aspirin versus aspirin-alone. Before therapy, patients in each treatment group had an increased percentage of B cells, and a decreased percentage of T cells, CD4 T cells, CD8 T cells and CD5+ B cells. There was no significant difference in immunologic parameters between the two groups measured before therapy. Patients treated with IVIG-and-aspirin had by the fourth day developed a highly-significant increase in T cells, CD4 T cells and CD8 T cells and a decrease in B cells. Despite the decrease of B cells, there were significant increases in CD5+ B cells in both treatment groups. However, the degree of increase in the IVIG-and-aspirin treated group was significantly more noticeable than that in the aspirin-alone treated group. These findings indicate that treatment with IVIG restores the T- and B- cell abnormalities, especially CD5+ B-cell abnormalities found in patients with acute KD.