RESUMO
Gene editing technology CRISPR/Cas9 and its derivative editing technologies including base editor and prime editor can precisely edit the target genome sequences, having been widely used in tumor therapy and achieved remarkable clinical results in tumor immunotherapy, human papilloma virus infection treatment and oncolytic virotherapy, providing a new means for tumor therapy.
RESUMO
CRISPR/ Cas9 gene editing system is a new tool of gene editing technology based on the im-mune mechanism of archaea against foreign nucleic acid invasion. Due to its high efficiency and accuracy, CRISPR/ Cas9 genome editing technology has been widely used in tumor therapeutic research,such as targeted knockout of oncogenes,repair of tumor suppressor genes,breaking immune tolerance,and construction of tumor models,which brings revolutionary development to tumor gene therapy.
RESUMO
Clustered regulatory interspaced short palindromic repeat (CRISPR )/ CRISPR-associated 9 (Cas 9) genome editing system is a new tool that is able to edit the cellular genome guided by RNA to recognize and edit DNA, providing the possibility to more efficient gene editing, which enables researchers to precisely manipulate specific genomic elements at the base level. Moreover, CRISPR /Cas 9 genome editing system has been widely used in cancer research. This review summarizes the molecular mechanism of the CRISPR /Cas 9 genome editing system, and introduces its application in cancer research and potential therapy in clinical practice based on the latest research.