Effect of irrigation at critical stages on the phenology of flowering and fruiting of the cactus Opuntia spp / Efeito da irrigação em estágios críticos sobre a fenologia de floração e frutificação do cacto Opuntia spp

Abstract This paper briefly reports some effects of irrigations at two critical periods on the phenology of three varieties of cactus pear cultivated in Agadir area: the spineless varieties 'Aissa' and 'Moussa' and the thorny one 'Achefri'. In the first year experiments (2010-2011) treatments of irrigation used were: (T1) 0 mm, (T2) 30 mm during flowering and 30 mm during fruit enlargement and (T3) 30 mm only during fruit enlargement. In the 2nd year experiments, irrigation treatments were: (T1) 0 mm, (T2) 60 mm during flowering and 60 mm during fruit enlargement and (T3) 60 mm only during fruit enlargement. Treatments of irrigation were applied between mid-April and mid-June in the 1st year experiments and in February and May in the 2nd year experiments. Results of the first year experiments showed that the emission of buds was higher in the thorny variety than in the spineless ones (more than 6 emitted buds/cladode vs less than 4.5 in the spineless varieties). In the second year, irrigation increased the emission of buds in the three varieties (more than 7 emitted buds/cladode for each T2 and T3 of all varieties vs not more than 5 for T1) and the duration of the flowering phase of these varieties. However, irrigation did not modify the proportions of fruits reaching commercial maturity during the early or the late period of maturation.

Resumo Este artigo relatou brevemente alguns efeitos das irrigações em dois períodos críticos na fenologia de três variedades de Opuntia cultivadas na área de Agadir: as variedades sem espinhos 'Aissa' e 'Moussa' e um espinhoso 'Achefri'. No primeiro ano (2010-2011) os seguintes tratamentos do experimento da irrigação foram usados: (T1) 0 milímetros, (T2) 30 milímetros durante a florescência e 30 milímetros durante a ampliação do fruto e (T3) 30 milímetros somente durante a ampliação do fruto. No segundo ano das experiências, os tratamentos da irrigação foram: (T1, 0 milímetros), (T2) 60 milímetros durante a florescência e 60 milímetros durante a ampliação do fruto e (T3) 60 milímetros somente durante a ampliação do fruto. Os tratamentos da irrigação foram aplicados entre a metade de abril e metade de junho nas experiências do primeira ano e em fevereiro e maio nas experiências do segundo ano. Os resultados das experiências do primeiro ano mostraram que a emissão de brotos foi maior na variedade espinhosa do que no rufia (mais de 6 emitida gomos/cladode vs inferior a 4,5 no rufia variedades). No segundo ano, a irrigação aumentou a emissão dos botões nas três variedades (mais de 7 botões emitidos/cladÓdio para cada T2 e T3 de todas as variedades não mais que 5 para o T1) e a duração da fase de florescência destas variedades. Contudo, a irrigação não alterou as proporções de frutos que alcançaram a maturidade comercial durante o período adiantado ou atrasado de maturação.

Seasonal variability of phenolic compounds and antioxidant activity in prickly pear cladodes of Opuntia and Nopalea genres

Abstract The prickly pear is a cactaceae that have chemical compounds that act as natural antioxidants. In addition, the prickly pear is a plant widely used in semiarid region as it's considered very important forage for animal feed, especially in the dry season. The objectives of this research were to characterize the main classes of phytochemicals, determine the phenols content, flavonoids and anthocyanins and evaluate the antioxidant activity of the prickly pear cultivars Opuntia and Nopalea grown in the semiarid region of Pernambuco in two collection periods (dry and rainy). In the work crude ethanol extracts from cladodes of cultivars (IPA-20, Elephant Ear Mexican, F-08, V-19, Small palm, F-21 and IPA-Sertânia) were used. As for the class of phytochemical compounds from ethanol extract only the flavonoids and steroids were detected in all prickly pear cultivars, in both periods analyzed. The content of phenolic compounds ranges from 1.24 to 5.41 mg GAE g-1 DM, flavonoids 0.90 to 3.43 mg QE g-1 DM, anthocyanins from 0.05 to 0.34 µg QE 100 g-1 DM and antioxidant activity 39.59 to 217.17 µM TEAC g-1 DM. The occurrence of chemical variability and antioxidant activity were observed, both among the botanical cultivars studied and among the collection periods.

Effect of irrigation at critical stages on the phenology of flowering and fruiting of the cactus Opuntia spp

Abstract This paper briefly reports some effects of irrigations at two critical periods on the phenology of three varieties of cactus pear cultivated in Agadir area: the spineless varieties Aissa and Moussa and the thorny one Achefri. In the first year experiments (2010-2011) treatments of irrigation used were: (T1) 0 mm, (T2) 30 mm during flowering and 30 mm during fruit enlargement and (T3) 30 mm only during fruit enlargement. In the 2nd year experiments, irrigation treatments were: (T1) 0 mm, (T2) 60 mm during flowering and 60 mm during fruit enlargement and (T3) 60 mm only during fruit enlargement. Treatments of irrigation were applied between mid-April and mid-June in the 1st year experiments and in February and May in the 2nd year experiments. Results of the first year experiments showed that the emission of buds was higher in the thorny variety than in the spineless ones (more than 6 emitted buds/cladode vs less than 4.5 in the spineless varieties). In the second year, irrigation increased the emission of buds in the three varieties (more than 7 emitted buds/cladode for each T2 and T3 of all varieties vs not more than 5 for T1) and the duration of the flowering phase of these varieties. However, irrigation did not modify the proportions of fruits reaching commercial maturity during the early or the late period of maturation.

Resumo Este artigo relatou brevemente alguns efeitos das irrigações em dois períodos críticos na fenologia de três variedades de Opuntia cultivadas na área de Agadir: as variedades sem espinhos 'Aissa' e 'Moussa' e um espinhoso 'Achefri'. No primeiro ano (2010-2011) os seguintes tratamentos do experimento da irrigação foram usados: (T1) 0 milímetros, (T2) 30 milímetros durante a florescência e 30 milímetros durante a ampliação do fruto e (T3) 30 milímetros somente durante a ampliação do fruto. No segundo ano das experiências, os tratamentos da irrigação foram: (T1, 0 milímetros), (T2) 60 milímetros durante a florescência e 60 milímetros durante a ampliação do fruto e (T3) 60 milímetros somente durante a ampliação do fruto. Os tratamentos da irrigação foram aplicados entre a metade de abril e metade de junho nas experiências do primeira ano e em fevereiro e maio nas experiências do segundo ano. Os resultados das experiências do primeiro ano mostraram que a emissão de brotos foi maior na variedade espinhosa do que no rufia (mais de 6 emitida gomos/cladode vs inferior a 4,5 no rufia variedades). No segundo ano, a irrigação aumentou a emissão dos botões nas três variedades (mais de 7 botões emitidos/cladÓdio para cada T2 e T3 de todas as variedades não mais que 5 para o T1) e a duração da fase de florescência destas variedades. Contudo, a irrigação não alterou as proporções de frutos que alcançaram a maturidade comercial durante o período adiantado ou atrasado de maturação.

Biology, Flowering and Fruiting of the Cactus Opuntia spp.: A Review and Some Observations on Three Varieties in Morocco

ABSTRACT The cactus is a succulent plant resistant to droughts. According to the recently reviewed classification, cacti belong to the family of Opuntiaceae Desv. (synon. Cactaceae Juss.) with Opuntia Mill. as the typical genus. This genus is economically the most important in the family, as it includes a group of cactus pear plants which play an important role in the agricultural systems of arid and semi-arid regions. Flowering of the cactus pear fruit is an important determinant of the fruit harvesting period. The goal of this paper is to present the physiology of the cactus pear and to explain in detail the biology of its flowering and fruiting processes. This study is also enriched by our observations on the flowering and fruiting of three varieties of cactus pear that we followed for two successive years in southern Morocco.

Capacidad antioxidante del fruto de la opuntia apurimacensis (ayrampo) y de la opuntia ficus-indica (tuna) / Antioxidant properties of the fruits opuntia apurimacensis (ayrampo)and opuntia ficus-indica (cactus pear)

Introducción. En la tuna existen compuestos antioxidantes, pero, se desconoce si hay en el ayrampo. Objetivo. Determinar la capacidadantioxidante de Opuntia apurimacensis (ayrampo) y de Opuntia ficus-indica (tuna). Diseño. Estudio analítico observacional. Lugar.Laboratorio de Bioquímica Clínica y Nutricional, Centro de Investigación de Bioquímica y Nutrición, Facultad de Medicina, UniversidadNacional Mayor de San Marcos, Lima, Perú. Material biológico. Fruta fresca (ff) de Opuntia apurimacensis (ayrampo) y Opuntia ficusindica(tuna). Intervenciones. En los extractos acuosos se realizaron determinaciones de vitamina C, polifenoles totales y capacidadantioxidante (FRAP). Los resultados se analizaron mediante la prueba de Mann-Whitney, con 95% de confianza. Principales medidasde resultados. Concentración de vitamina C y polifenoles totales; capacidad antioxidante. Resultados. El ayrampo presentó mayorconcentración de vitamina C (49,9 y 36,1 mg de ácido ascórbico/100 g ff), mayor concentración de polifenoles totales (107,3 y68,7 mg de equivalente ácido gálico/100 g ff) y mayor capacidad antioxidante (1,1 y 0,7 mmoles de Fe- II/100 g ff) que la tuna. Elayrampo mostró mayor correlación entre la capacidad antioxidante y la vitamina C (0,91 y 0,56), mientras que, la tuna tuvo mayorcorrelación entre la capacidad antioxidante y los polifenoles totales (0,98 y 0,82). Conclusiones. El ayrampo presentó mayor capacidadantioxidante que la tuna.

Introduction. The cactus pear contains antioxidant compounds, but it is unknown if these exist in the ayrampo. Objective. To determinethe antioxidant capacity of Opuntia apurimacensis (ayrampo) and Opuntia ficus-indica (cactus pear). Design. Analytical observationalstudy. Location. Laboratory of Clinical Biochemistry and Nutrition, Research Center of Biochemistry and Nutrition, Faculty of Medicine,Universidad Nacional Mayor de San Marcos, Lima, Peru. Biological material. Fresh fruit (ff) of both Opuntia apurimacensis (ayrampo)and Opuntia ficus-indica (cactus pear). Interventions. Determination of vitamin C, polyphenols and antioxidant capacity (FRAP) wereperformed in both fruits aqueous extracts. Results were analyzed using the Mann-Whitney U test with 95% confidence. Main outcome measures. Concentration of vitamin C and polyphenols; antioxidant capacity (FRAP). Results. Compared to the cactus pear, ayrampopresented the highest concentration of vitamin C (49.9 and 36.1 mg ascorbic acid/100 g ff), highest concentration of total polyphenols(107.3 and 68.7 mg gallic acid equivalent/100 g ff) and highest antioxidant capacity (1.1 and 0.7 mmol of Fe-II/100 g ff). Ayramposhowed the highest correlation between antioxidant capacity and vitamin C (0.91 and 0.56), and the prickly pear the higher correlationbetween antioxidant capacity and total polyphenols (0.98 and 0.82). Conclusions. Ayrampo showed higher antioxidant capacitycompared to cactus pear.

Effect of ultrasound on survival and growth of Escherichia coli in cactus pear juice during storage

Abstract The aim of this study was to investigate the effectiveness of ultrasound as a conservation method for the inactivation of Escherichia coli inoculated into cactus pear juices (green and purple). Total soluble solids, pH, titratable acidity, and the kinetics of E. coli in cactus pear juices treated by ultrasound (60%, 70%, 80% and 90% amplitude levels for 1, 3 and 5 min) were evaluated over 5 days. Total inactivation was observed in both fruit juices after 5 min of ultrasound treatment at most amplitude levels (with the exception of 60% and 80%). After one and two days of storage, the recovery of bacteria counts was observed in all cactus pear juices. Ultrasound treatment at 90% amplitude for 5 min resulted in non-detectable levels of E. coli in cactus pear juice for 2 days. The parameters of pH, titratable acidity and soluble solids were unaffected.

Evaluation of protective effect of cactus pear seed oil (Opuntia ficus-indica L. MILL.) against alloxan-induced diabetes in mice

Objective: To evaluate the in vitro antioxidant power of cactus pear seed oil [. Opuntia ficus-indica L. MILL. (CPSO)] and its protective effect against chemically induced diabetes mellitus in mice. Methods: The in vitro antioxidant effect of CPSO was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. The preventive effect was conducted on Swiss albino mice treated with CPSO (2 mL/kg, per os), before and after a single intraperitoneal alloxan administration (100 mg/kg). Survival rate, body weight and fasting blood glucose were measured and histopathological analysis of pancreas was performed to evaluate alloxan-induced tissue injuries. Results: CPSO exhibited an antioxidant effect in DPPH scavenging assay. Moreover, the administration of CPSO (2 mL/kg) significantly attenuated alloxan-induced death and hyperglycemia (P < 0.001) in treated mice. Morphometric study of pancreas revealed that CPSO significantly protected islets of langerhans against alloxan induced-tissue alterations. Conclusions: Based on theses results, CPSO can prevente alloxan-induced-diabetes by quenching free radicals produced by alloxan and inhibiting tissue injuries in pancreatic β-cells.

Evaluation of protective effect of cactus pear seed oil (Opuntia ficus-indica L. MILL.) against alloxan-induced diabetes in mice

OBJECTIVE@#To evaluate the in vitro antioxidant power of cactus pear seed oil [Opuntia ficus-indica L. MILL. (CPSO)] and its protective effect against chemically induced diabetes mellitus in mice.@*METHODS@#The in vitro antioxidant effect of CPSO was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. The preventive effect was conducted on Swiss albino mice treated with CPSO (2 mL/kg, per os), before and after a single intraperitoneal alloxan administration (100 mg/kg). Survival rate, body weight and fasting blood glucose were measured and histopathological analysis of pancreas was performed to evaluate alloxan-induced tissue injuries.@*RESULTS@#CPSO exhibited an antioxidant effect in DPPH scavenging assay. Moreover, the administration of CPSO (2 mL/kg) significantly attenuated alloxan-induced death and hyperglycemia (P < 0.001) in treated mice. Morphometric study of pancreas revealed that CPSO significantly protected islets of langerhans against alloxan induced-tissue alterations.@*CONCLUSIONS@#Based on theses results, CPSO can prevente alloxan-induced-diabetes by quenching free radicals produced by alloxan and inhibiting tissue injuries in pancreatic β-cells.