RESUMO
Objective: To screen the optimum conditions of proliferation, regenerated bud differentiation, and adventitious roots induction of Asarum heterotropoides petiole-derived callus so as to establish the effective system for callus proliferation and regeneration. Methods: Petiole-derived calli were subcultured in the proliferation medium supplemented with different concentration of 6-BA and NAA. Then green and compact calli were chosen to culture in differentiation medium with different exogenous hormones for the induction of regeneration bud and adventitious roots. After being acclimated, regenerated plants were transplanted. Results: The effective callus proliferation medium was 1/2 MS supplemented with 0.40 mg/L 6-BA and 0.10 mg/L NAA, then 1/2 MS medium supplemented with 0.4 mg/L 6-BA and 0.05 mg/L NAA was suitable for regenerated bud differentiation while regenerated buds grew better in 1/2 MS medium without any hormone. The medium of 1/4 MS supplemented with 0.25 mg/L IBA was best for the induction of adventitious roots. Conclusion: The optimum culture conditions of callus proliferation and differentiation in A. heterotropoides are determined to establish the regeneration system and obtain the regenerated plantlet successfully.