Expressions and significance of CIP2A and VEGF in hepatocellular carcinomas / 中国医师杂志

Objective To investigate the expressions of cancerous inhibitor of protein phosphatase 2A (CIP2A) and vascular endothelial growth factor (VEGF) in hepatocellular carcinomas and their clinical significance.Methods The expressions of CIP2A and VEGF proteins were tested with immunohistochemistry in 60 cases of hepatocellular carcinomas and adjacent paracancerous tissues.Results The positive rate of CIP2A in hepatocellular carcinomas was significantly higher than adjacent paracancerous tissues (83.3％ vs 9.5％,P ＜ 0.05).Significant differences were also observed in the expression rate of VEGF between the patients with hepatocellular carcinomas and paracancerous tissues (75.0％ vs 14.3％,P ＜0.05).The expression of CIP2A in hepatocellular carcinomas was significantly positively correlated with its pathological grading,differentiation,and tumor node metastasis (TNM) stage (P ＜ 0.05).The expression of VEGF in hepatocellular carcinomas was significantly positively correlated with its pathological grading,differentiation,and TNM stage (P ＜ 0.05).Significantly positive correlation was found between expressions of CIP2A and VEGF with Spearman correlation analysis (rs =0.465,P ＜ 0.01).Conclusions The abnormal expressions of CIP2A and VEGF gene may promote tumor angiogenesis and progression of a hepatocellular carcinoma.The study supports positive regulation between expressions of CIP2A and VEGF in a hepatocellular carcinoma.

The expression and clinical significance of MMP-9 in hepatocellular carcinoma / 中国医师杂志

Objective To study the expression of MMP-9 in epatocellular carcinoma (HCC) and the relationship between its expression and clinical pathological characteristics.Methods Specimens taken from 67 fresh resected HCC and 16 normal liver tissues were selected.Tissue sections were stained by EnVisionTM immunohistochemistry with anti-MMP-9 monoclonal antibodies.The expression of MMP-9 in HCC and the relationship between its expression and clinical pathological characteristics were analyzed with x2test,t test and rank-sum test.Results The positive ratio of MMP-9 in HCC tissues was 58.21％,it was obviously higher than that of normal liver tissues (12.5％,x2 =10.80,P ＜ 0.01).The expression of MMP-9 in the metastatic HCC tissues was significantly higher than that in the non-metastatic HCC tissues (40.18 vs 26.38,P ＜0.01).Conclusions The positive ratio of MMP-9 expression revealed that the expression of MMP-9 increased obviously in the HCC,and that its expression in the metastatic HCC tissues significantly increased relative to the non-metastatic tissues.It suggests that MMP-9 might decompose and destroy the extracellular matrix and cell membrane around HCC cell surface,and then promote invasion and metastasis of HCC.