In vitro anticancer activity of cardiospermum halicacabum (linn) against hepatocellular carcinoma cell line (hep-g2).

Cardiospermum halicacabum is important medicinal plant which belongs to Sapindaceae family.In present study, ethanolic leaf extract of Cardiospermum halicacabum was examined for its anticancer activity against Hepatocellular carcinoma cell line (Hep-G2) using MTT assay. The leaf extract shows significant cytotoxic effect with 90% of inhibition when compared to standard drug.

Flavonoids of Zhuang medicine Cardiospermum halicacabum / 中草药

Objective To study the chemical constituents of Zhuang medicine Cardiospermum halicacabum. Methods The compounds were isolated and purified by silica gel, polyamide gel, and Sephadex LH-20 chromatography. The structures of the compounds were identified on the basis of chemical and spectral methods. Results Thirteen compounds were isolated from the butanol extracts of C. halicacabum and identified as chrysoeriol-7-O-β-D-glucuronide butyl ester (1), chrysoeriol-7-O-β-D- glucuronide ethyl ester (2), chrysoeriol-7-O-β-D-glucuronide methyl ester (3), chrysoeriol (4), apigenin-7-O-β-D-glucuronide butyl ester (5), apigenin-7-O-β-D-glucuronide ethyl ester (6), apigenin-7-O-β-D-glucuronide methyl ester (7), apigenin-7-O-β-D- glucuronide (8), apigenin (9), luteolin-7-O-β-D-glucuronide methyl ester (10), luteolin-7-O-β-D-glucuronide (11), luteolin (12), and quercetin (13). Conclusion Compounds 1, 2, and 3 are new flavonoiels named as cardiosperoside D, cardiosperoside B, and cardiosperoside A. Compounds 5-8, 10, and 11 are isolated from this genus for the first time.

Study on the Spectrum-effect Relationship of Antioxidant Activity in vitro of Cardiospermum halicacabum from Guangxi / 中国药房

OBJECTIVE:To establish the fingerprints of 11 batches of Cardiospermum halicacabum from Guangxi,and detect its spectrum-effect relationship of antioxidant activity in vitro,and provide reference for revealing the material basis for its antioxi-dant activity. METHODS:HPLC was conducted to establish the fingerprints of 11 batches of C. halicacabum from Guangxi,the 1, 1-diphenyl-2-trinitrobenzene hydrazine(DPPH)free radical scavenging method was used to determine the antioxidant activity in vi-tro of C. halicacabum from different origins,and bivariate correlation analysis was adopted to detect the spectrum-effect relation-ship of antioxidant activity in vitro. RESULTS:The similarities of 11 batches of C. halicacabum from Guangxi were all more than 0.9,and the content change of peak 2(protocatechuic acid),3,4,12 in 16 common peaks was closely related to the DPPH free radical scavenging activity. CONCLUSIONS:The chemical composition of C. halicacabum from different origins in Guangxi is ba-sically consistent,while there are some differences in the quality of the producing area. The protocatechuic acid (peak 2) and the unknown compounds represented by peak 3,4,12 may be the pharmacodynamic material basis of its antioxidant activity.

Formulation and evaluation of topical herbal gel for the treatment of arthritis in animal model

ABSTRACT The objective of the study is to formulate and evaluate a topical herbal gel containing Cardiospermum halicacabum and Vitex negundo leaf extracts for their anti-arthritic activity in rats. Twelve herbal gel formulations were prepared using 1.5% of gelling agents carbopol 934 (F1-F6) and carbopol 940 (F6-F12) and they were evaluated for physical appearance, net content, viscosity, extrudability, pH, spreadability, in vitro diffusion profile and primary skin irritation tests. The stability study for the topical herbal gel formulation was done as per ICH guidelines and anti-arthritic activity was evaluated by Freund's Complete Adjuvant (FCA) induced arthritis method. Assessment of body weight, paw volume, hematological and biochemical parameters, histopathological examination and In vitro determination of serum biomarkers were also carried out. Formulated gels were homogenous, stable and complied with the guidelines. Among the formulations, F4 showed better release (98.4 %) characteristics than other formulations. No erythema or edema was observed in the skin irritation test confirming the gel was non-toxic and safe. Topical application of the herbal gel F4 containing carbopol 934 displayed significant (p < 0.001) anti-arthritic activity compared to diseased rats. Reduction in paw volume, no agglutination in C - reactive protein and rheumatic factor, reduction in TNF level, regaining of normal hematological, and biochemical parameters, reduction in spleen and thymus weight and histopathological examination supported the anti-arthritic activity of the gel formulation.

In vitro and In vivo Anti-Arthritic Activity of Combined Ethanolic Extracts of Pisonia grandis and Cardiospermum halicacabum in Wistar Rats.

The combined Bi-herbal ethanolic extract (BHE) made up of equal quantities of leaves of Pisonia grandis and Cardiospermum halicacabum was evaluated for its synergistic in vitro and in vivo Anti-arthritic effects and was compared with its individual ethanolic extracts of Pisonia grandis (EPG) and Cardiospermum halicacabum (ECH). The inhibition of protein denaturation, membrane stabilization and proteinase enzyme inhibition were studied as in vitro and Freund’s adjuvant arthritis model in Wistar rats was used to study the in vivo Anti-arthritic activity. The three extracts (BHE, EPG, and ECH) at the dose level of 300mg/kg b.w.in 2%CMC were ingested orally to the CFA treated rats once daily for 40 days and the percentage inhibition of the paw volume increase due to the drug treatment was calculated. All the three plant extracts exhibited dose dependent stabilization of the RBC membranes, inhibition of protein denaturation and protease inhibition. The BHE has got a significant reduction in the paw volume and oedema when comparable to EPG and ECH in these intoxicant treated animals. These observed effects may be due to its inhibition of free radical production due to the immunological and inflammatory reactions or minimizing protein denaturation or proteinase enzyme or stabilizing the membranes.

In Vitro Antioxidant Studies Of Cardiospermum Halicacabum L. Var. Luridum (Blume) Adelb Seeds.

Cardiospermum halicacabum Linn. (Sapindaceae) is a climber, commonly found throughout India, used as vegetable as well as traditional medicine for rheumatism, lumbago and nervous diseases. In the current study, we investigated the antioxidant potency of successive extracts of C. halicacabum seeds employing various established in vitro systems, such as the lipid peroxidation, nitric oxide radical scavenging and reduced glutathione assay. Furthermore, all the extracts exhibited concentration dependent inhibition in all the models at tested doses 25-1000 μg/ ml. The data obtained in the in-vitro models clearly establish the antioxidant potency of C. halicacabum seed extract.

Anticonvulsant activity of alcoholic root extract of Cardiospermum halicacabum

The aim of the present study was to evaluate the anticonvulsant effects of alcoholic root extract of Cardiospermum halicacabum L., Sapindaceae (ARECH), on the various murine models of epilepsy. The root extract of the plant was administered p.o. to male swiss albino mice at doses of 30, 100 and 300 mg/kg before evaluation. The brain monoamine levels were determined after two days administration. ARECH at doses of 100 and 300 mg/kg significantly delayed the onset of clonus and tonus in pentylenetetrazol, isoniazid and picrotoxin-induced convulsions. Tonic hind limb extension was also decreased at doses of 100 and 300 mg/kg as compared to vehicle control in maximal electroshock model. No significant motor toxicity was observed even at a highest dose administered, i.e. 900 mg/kg. Brain monoamine analysis by HPLC revealed a significant increase in GABAergic activity in C+ (in cerebellum) and C- (except cerebellum). These results suggested that ARECH possesses a significant anticonvulsant activity with a low motor toxicity profile. This activity may be attributed to an increase in GABAergic activity.

Seed-carrying and feeding behavior of Jadera choprai Gõllner-Scheiding (Heteroptera: Rhopalidae) / Comportamento de carregamento de semente e alimentação de Jadera choprai Gõllner-Scheiding (Heteroptera: Rhopalidae)

Durante os meses de fevereiro e março de 2001, observou-se o comportamento de carregamento de sementes de Cardiospermum halicacabum (L.) (Sapindaceae) e alimentação pelo percevejo Jadera choprai Gõllner-Scheiding . Observações de campo revelaram que o percevejo se alimenta de sementes maduras caídas no solo. Testes conduzidos em laboratório indicaram que o percevejo é capaz de conduzir as sementes por distâncias de até 200 cm. Vários indivíduos foram observados alimentando-se simultâneamente de uma única semente, com ocorrência de canibalismo. Aparentemente, trata-se da primeira contatação da associação de J. choprai com C. halicacabum e do primeiro registro do carregamento de sementes maduras por essa espécie de ropalídeo.

During February-March 2001, the seed-carrying and feeeding behavior on Cardiospermum halicacabum (L.) (Sapindaceae) by Jadera choprai Gõllner-Scheiding was studied. Field observations indicated that the bugs feed on seeds found on the ground. Laboratory studies indicated that J. choprai may carry seeds 200 cm from where they were found. Several individuals were observed feeding on one seed, with the occurrence of cannibalism. Apparently, this is the first record on the association of J. choprai with C. halicacabum, and on seed-carrying by this rhopalid.