Chelation activity and protective effect of Caulerpa lentillifera aqueous extract against lead acetate-induced toxicity in Sprague Dawley rats

Lead is a multisystemic toxicant when it accumulates and is hazardous to health. This study was conducted to determinethe effect of Caulerpa lentillifera aqueous extract on lead accumulation in the internal organs and liver functions oflead-intoxicated rats. Sprague Dawley rats (n = 24) were divided into four groups (n = 6). Group I was healthy ratstreated with saline (2 ml/kg bwt) daily for 28 days. Group II was healthy rats treated with C. lentillifera aqueousextract (500 mg/kg bwt) daily for 28 days. Group III was intoxicated with lead acetate (20 mg/kg bwt) daily for 4 daysand then treated with saline (2 ml/kg bwt) for another 24 days. Group IV was intoxicated with lead acetate (20 mg/kgbwt) daily for 4 days and then treated with C. lentillifera aqueous extract (500 mg/kg bwt) for another 24 days. Leadaccumulation in the internal organs was measured by the atomic absorption spectrophotometer and the liver functionmarkers were determined using assay kits. Lead intoxication significantly (p < 0.05) increased lead accumulation inthe rats’ internal organs and affected their liver functions. C. lentillifera aqueous extract supplementation to leadintoxicated rats significantly (p < 0.05) decreased lead accumulation in the rats’ internal organs and protected theirliver functions. This study concludes that C. lentillifera aqueous extract possesses the capability of a chelating agentand attenuates the effect of lead on lead-intoxicated rats

Caulerpa lentillifera and Sucrose Formulated Diets as Growth Enhancer of Oyster (Crassostrea virginica)

A total of 200 of Crassostrea virginica populations of average weight range from 41.19 ± 3.42 to 47.53 ± 1.06 g were studied to determine the effect of the feed diets of Caulerpa lentillifera and sucrose as growth enhancement. Growth rates increased that range from 56.99 ± 3.16 to 61.56 ± 2.87 g for 90 days period using an artificial water tank system. Previous studies conducted that C. lentillifera contained high protein and were the most abundant component. This seaweed also contained high amounts of minerals and balanced amino acid and notably very rich in iodine. phosphorus, calcium, magnesium and copper that will contribute to oysters growth. Oysters are known to have the ability to uptake dissolved organic matter as nutrients. In the present study, the effects of culture water supplemented with sucrose were tested on oysters. Results revealed that this organic matter promotes growth to the oysters. Sugars will be metabolized into pyruvate through the glycolysis pathway and will result in the supply of energy. Therefore, supplementation of sugar to oysters may have contributed as an energy source together with the lipid and protein content from the algae diet.

Anti-diabetic effects of Caulerpa lentillifera：stimulation of insulin secretion in pancreatic β-cells and enhancement of glucose uptake in adipocytes

Objective: To evaluate anti-diabetic effect of Caulerpa lentillifera (C. lentillifera).Methods:The inhibitory effect of C. lentillifera extract on dipeptidyl peptidase-IV andα-glucosidase enzyme was measured in a cell free system. Then, interleukin-1β and interferon-γinduced cell death and insulin secretion were measured in rat insulinoma (RIN) cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and ELISA kit, respectively. Glucose uptake and glucose transporter expression were measured by fluorometry and western blotting, using 3T3-L1 adipocytes.Results: C. lentillifera extract significantly decreased dipeptidyl peptidase-IV and α-glucosidase enzyme activities, and effectively inhibited cell death and iNOS expression in interleukin-1βand interferon-γ induced RIN cells. Furthermore, C. lentillifera extract significantly enhanced insulin secretion in RIN cells and glucose transporter expression and glucose uptake in 3T3-L1 adipocytes.Conclusions:Thus, our results suggest that C. lentillifera could be used as a potential anti-diabetic agent.

Anti-diabetic effects of Caulerpa lentillifera: stimulation of insulin secretion in pancreatic β-cells and enhancement of glucose uptake in adipocytes

<p><b>OBJECTIVE</b>To evaluate anti-diabetic effect of Caulerpa lentillifera (C. lentillifera).</p><p><b>METHODS</b>The inhibitory effect of C. lentillifera extract on dipeptidyl peptidase-IV and α-glucosidase enzyme was measured in a cell free system. Then, interleukin-1β and interferon-γ induced cell death and insulin secretion were measured in rat insulinoma (RIN) cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and ELISA kit, respectively. Glucose uptake and glucose transporter expression were measured by fluorometry and western blotting, using 3T3-L1 adipocytes.</p><p><b>RESULTS</b>C. lentillifera extract significantly decreased dipeptidyl peptidase-IV and α-glucosidase enzyme activities, and effectively inhibited cell death and iNOS expression in interleukin-1β and interferon-γ induced RIN cells. Furthermore, C. lentillifera extract significantly enhanced insulin secretion in RIN cells and glucose transporter expression and glucose uptake in 3T3-L1 adipocytes.</p><p><b>CONCLUSIONS</b>Thus, our results suggest that C. lentillifera could be used as a potential anti-diabetic agent.</p>

Anti-diabetic effects of Caulerpa lentillifera: Stimulation of insulin secretion in pancreatic ß-cells and enhancement of glucose uptake in adipocytes

Objective: To evaluate anti-diabetic effect of Caulerpa lentillifera (C. lentillifera). Methods: The inhibitory effect of C. lentillifera extract on dipeptidyl peptidase-IV and α-glucosidase enzyme was measured in a cell free system. Then, interleukin-1ß and interferon-γ induced cell death and insulin secretion were measured in rat insulinoma (RIN) cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and ELISA kit, respectively. Glucose uptake and glucose transporter expression were measured by fluorometry and western blotting, using 3T3-L1 adipocytes. Results: C. lentillifera extract significantly decreased dipeptidyl peptidase-IV and α-glucosidase enzyme activities, and effectively inhibited cell death and iNOS expression in interleukin-1ß and interferon-γ induced RIN cells. Furthermore, C. lentillifera extract significantly enhanced insulin secretion in RIN cells and glucose transporter expression and glucose uptake in 3T3-L1 adipocytes. Conclusions: Thus, our results suggest that C. lentillifera could be used as a potential anti-diabetic agent.