Human Papillomavirus 16/18 Expression of Endocervical Glandular Lesions: Relationship with p53 and MIB-1 Expressions

The pathogenesis of endocervical glandular lesions are not clearly understood. The aims of this study are to evaluate the etiologic role of human papillomavirus (HPV) 16/18 and the relationship of HPV 16/18, p53 and MIB-1 expressions in endocervical glandular dysplasia (EGD), adenocarcinoma in situ (AIS) and adenocarcinoma. The materials included 14 endocervical adenocarcinoma and 5 AIS and 18 high grade EGD and 39 low grade EGD. Immunohistochemistry for p53 and MIB-1, and in situ PCR for HPV 16/18 were done. HPV 16/18 positivity was 84.2%, 16.7% and 17.9% in malignant glandular lesion (adenocarcinoma and AIS), high grade EGD and low grade EGD, respectively. P53 protein expression rates of malignant glandular lesions, high grade EGD and low grade EGD were 31.6%, 11.1%, and 0%, respectively. High MIB-1 labelling index was found in 73.7% of malignant glandular lesions, but in only 5.7% and 3.6% of high and low grade EGD, respectively. There were statistically significant differences in HPV 16/18, p53 and MIB-1 expressions between malignant endocervical glandular lesions and EGD, but no significant difference in p53 and MIB-1 expressions in relation to HPV 16/18 expression. In malignant endocervical glandular lesions, HPV 16/18 infection may be a major causative factor, but not be related to p53 and MIB-1 expressions.

Avaliação da recidiva de neoplasias intra-epiteliais cervicais uterinas tratadas por eletrocirurgia de alta freqüência / Evaluation of recurrent cervix intra-epitelial neoplasms treated by high frequency eletrosurgery

Os autores apresentam os resultados do tratamento das neoplasias intra-epiteliais cervicais em 20 pacientes, através da conização eletrocirúrgica do colo uterino com bisturi de alta freqüência. A proposta foi avaliar a incidência de recidiva das lesões com este método terapêutico, seis meses após a intervenção. Ao final, 82,4 por cento das pacientes apresentaram exame citológico cervical normal. Assim, recomendam a eletrocirurgia com bisturi de alta freqüência, para o tratamento dessas lesões, uma vez que apresenta baixos índices de recidiva e vantagens em relação à cirurgia convencional, como facilidade técnica, possibilidade de execução em nível ambulatorial, sob anestesia loca, rapidez e baixo custo operacional, entre outros.

The authors present the recurrence rate of conization in the treatment of cervix intra-epitelial neoplasms by the high frequency electrosurgery technique. A 82.4% of normal citology were observed six months postoperatively. The method is recomended because it is associated with low rates of recurrence, besides being easily performed, under local anesthetics, saving time and costs, in an outpatient program.