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OBJECTIVE To identify the chemical components of Changtong oral liquid （CTOL），and to provide reference for the basic research and secondary development of its pharmacological substances. METHODS UHPLC-Orbitrap HRMS technique was adopted. CTOL sample was separated on a Hypersil Gold column with mobile phase consisted of 0.1% formic acid （containing 5 mmol/L ammonium formate）-acetonitrile （gradient elution）. The eluent was detected in positive and negative ion modes using an electrospray ionization source. The data was processed by Xcalibur 4.3 and Compound Discoverer 3.3 software. The primary and secondary mass spectra data of each compound were collected. The unknown compounds were identified according to the mass spectrometry library of the instrument and the network databases mzCloud，mzVault，etc. Through matching with the pharmacology database and analysis platform of the traditional Chinese medicine system，the chemical components could be attributed to the traditional Chinese medicine. RESULTS Fifty-three chemical components were identified and analyzed from CTOL，such as 24 flavonoids，8 quinones，5 phenylpropanoids，4 sugars and glycosides，5 organic acids，3 amino acids，1 alkaloid，1 phenolic and 2 other compounds. Among them，12 components were derived from Salvia miltiorrhiza，9 from Citrus aurantium，7 from Rheum palmatum，4 from Angelica sinensis，1 from Magnolia officinalis，16 from Glycyrrhiza uralensis，and 4 from many kinds of medicinal materials. CONCLUSIONS CTOL mainly contains flavonoids，quinones and phenylpropanoid compounds，and its chemical components mainly come from G. uralensis，S. miltiorrhiza and C. aurantium.
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Objective To determine the content of the heavy metal Cadmium(Cd)in Changtong oral liquid.Methods Inductively coupled plasma mass spectrometry(ICP -MS )was used.Results The calibration curve was linear in t he range of 0.0025～0.0125?g /mL for Cd(r=0.9997).The average recovery of the heavy me tal element of Cd was99.37%(n=5,RSD =2.73%).Conclusion This method is simple,accurate and e ffective.It can be used for the quality control of Changtong oral li quid.
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OBJECTIVE:To determine the content of emodin in Changtong oral liquid.METHODS:HPLC was used.The stationary phase was Nova-Pak C 18 column and the mobile phase was methanol-0.1%phosphoric acid solution(85∶15).The UV detection wavelength was254nm.RESULTS:There was a good linear relationship within the concentration range of0.48～2.4?g/ml of emodin in Changtong oral liquid(r=0.9998).The average recovery was99.73%(n=5),RSD=1.42%. CONCLUSION:This method is simple,fast and accurate.
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OBJECTIVE: To observe the preventive mechanism of Changtong oral liquid on postoperative intestinal adhesion .METHODS: The intestinal adhesion model was established by traumatizing cecal scrosa and ligating adjacent abdominal wall/The effect of Changlong oral liquid on the expression of TGF-?1 was examined by immunohistochemical method.RESULTS & CONCLUSION:Changtong oral liquid can decrease the expression of TGF - ?1 of rats with adhesion .Maybe it is one of the mechanism that Changtong oral liquid can prevent postoperative intestinal adhesion.
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Objective To observe the effect of Changtong Oral Liquid (CTOL) on serum TNF-? and IL-4 levels in rats with postoperative intestinal adhesion. Methods Sprague-Dawley (SD) male rats were randomly divided into six groups: normal control, no treatment control, Simo Oral Liquid (SOL) group, and CTOL group (minimal, medium and maximal dosages). Except for normal control group, intestinal adhesions were produced in all the other rats with the method of Ellis. The blood samples of rats with CTOL medium dosage and no treatment group were collected on the 1st, 3rd, 5th, 7th and 9th day, and that of the rest was drawn on day 7 after surgery for the determination of TNF-? and IL-4 levels. Results The serum TNF-? levels were higher in no treatment rats compared with the normal controls (P0.05). Conclusions TNF-? was closely related to adhesion formation, and it may be regarded as a marker of adhesion formation.