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OBJECTIVE:To compare the composition changes of Aurantii fructus before and after fermentation processing and optimize its fermentation processing technology. METHODS:UPLC was conducted to compare the raw and fermentation processed products of same batch of Aurantii fructus,and ensure the chromatographic peaks after fermentation processing. Using peak areas of 4 chromatographic peaks and mildew characteristics of samples as index,fermentation temperature,humidity and time as factor, L9(34)orthogonal test was designed to optimize the fermentation processing technology,and verified it. RESULTS:After fermenta-tion processing,Aurantii fructus obviously showed 2 new monosaccharide glycosides components;the optimized fermentation tech-nology was as follows as fermentation temperature of 30 ℃,humidity of 70% and time of 7 d;verification test results showed RSD of each indicator of decoction pieces prepared by optimized fermentation technology in 3 tests were lower than 2.0%(n=3). CONCLUSIONS:Fermentation processing may lead obvious chemical composition changes in Aurantii fructus;the optimized fer-mentation processing technology can increase the contents of characteristic peaks.
RESUMO
OBJECTIVE: To establish the HPLC characteristic fingerprints of Gentiana scabra Bge. and G. rigescens Franch. METHODS: The characteristic fingerprints were established based on polarity orientation technology. Similarity analysis, cluster analysis, and principal component analysis were used to evaluate the fingerprints. RESULTS: The HPLC fingerprints of ethyl acetate fraction from the water extract of G. scabra and G. rigescens showed obvious characteristics. The overall similarity of 21 batches of samples was 0.29-0.95. The overall similarity of G. scabra was 0.82-0.99 except S4. And that of G. rigescens was 0.75-0.99 among which 10 batches were greater than 0.90. In the cluster analysis, the samples were divided into three clusters: S4, G. scabra, and G. rigescens. In the principal component analysis, 21 batches of G. scabra. and G. rigescens could be clearly divided into two categories, while S4 was far away from the other samples of G. scabra. CONCLUSION: G scabra and G. rigescens can be identified effectively by the characteristic fingerprints. The method can be used for the quality control of Radix Gentianae.
RESUMO
Objective: To optimize the ethanol extraction process of Psoraleae Fructus-Myristicae Semen (psoralen-nutmeg) drug pair. Methods: Using L9(34) orthogonal design, the effects of ethanol concentration, ethanol amount, extraction time, and extraction times on the extraction process were investigated. The contents of psoralen, isopsoralen, and dehydrodiisoeugenol, dry extract yield, and total area of HPLC fingerprint characteristic peaks were used as comprehensive evaluation indexes. Results: The optimum process conditions were as follows: 50% ethanol, six times of the ethanol volume, extracted for three times, each time for 2 h. Conclusion: The method provides the basis for the determination of ethanol extraction process of Psoraleae Fructus-Myristicae Semen drug pair.