Primary research on components with anticomplement and antitussive activities from leave extracts of Chimonanthus nitens / 中草药

Objective: To investigate and analyze anticomplement and antitussive activities and the active ingredients of the extract of Chimonanthus nitens leaf. Methods: The classical anti-complement pathway and the concentrated ammonia-induced cough model was used to compare the activity of the different polar parts of C. nitens leaf, and the polar parts with anti-complement and antitussive activity were determined. A preliminary analysis of the chemical composition in activity extract was identified by high performance liquid chromatography-mass spectrometry. The main chemical components in the C. nitens leaf of antitussive and antibody activity were also evaluated. Results: The ethyl acetate extract of C. nitens leaf had both anti-complement and antitussive effects. A total of 28 compounds were initially identified through mass spectrometry analysis. Kaempferol-3-O-rutinoside and kaempferol had both antitussive and anti-complement activities. Conclusion: The ethyl acetate extract of C. nitens leaf has good anti-complement and antitussive activities, and the mainly active ingredients in it were kaempferol-3-O-rutinoside and kaempferol that could be used as quality-controlling chemical markers.

Comparative Study on the Contents of Four Compounds from Different Processed Products of Miao Medicine Chimonanthus nitens and Its Toxicity / 中国药房

OBJECTIVE： To compare the contents of 4 components and toxicity from Miao medicine Chimonanthus nitens samples after drying in the shade ，oven drying ，steam，charring. METHODS ：The contents of scopolin，fraxin，scopoletin and isofraxin were simultaneously determined by HPLC. The separation was performed on Welch-C18 column with mobile phase consisted of acetonitrile-water （gradient elution ）at the flow rate of 1 mL/min. The detection wavelength was set at 214 nm，and column temperature was 30 ℃，and the sample size was 10 μL. Modified Karber method was used to determine the LD 50 and its 95％CI of different processed C. nitens to mice and evaluate its acute toxicity. RESULTS ： The linear range of scopolin ， fraxin， scopoletin and isofraxin were 0.019-1.856，0.016-1.616，0.009-0.920，0.006-0.624 μg（R2 were all not lower than 0.999 5）. RSDs of precision ， stability and reprodu-cibility tests were all lower than 2.0％（n＝6）. The average recoveries were 104.49％，102.22％， 101.45％，99.26％（all RSDs were lower than 2％，n＝6）. The contents of scopolin were 1.119 0％，0.904 3％， 1.068 4％and 0.036 4％；those o f fraxin were 0.867 8％，0.453 9％，0.423 7％and 0.020 5％；those of scopoletin ； those of isofraxidin were 0.110 2％，0.202 1％，0.208 1％ and 0.249 4％in samples after drying in the shade ，oven CX-2018-001） drying， steam， charring. The LD 50 of 4 processedproducts were 4 118.13，3 733.36，1 643.61，＞10 000 qq.com mg/kg samples ；95％CI were （3 748.87，4 523.76）， （3 422.16，4 072.86），（1 520.90，1 776.23），（＞10 000） mg/kg. CONCLUSIONS ：HPLC method is reproducible and precise. It can be used to determine the contents of 4 components in different processed products of C. niten s. The contents of 4 components in different processed products ， and the contents of glycosides toxicity components are decreased significantly. All the 4 processed products were low or non-toxic.

Chemical Constituents of Chimonanthus nitens Oliv / 中国药学杂志

OBJECTIVE: To study the chemical constituents from the leaves of Jiangxi genuine medicinal material Chimonanthus nitens Oliv. METHODS: The compounds were isolated and purified by silica gel column chromatography, Sephadex LH-20, ODS column chromatography, semi-preparative HPLC, and so on. Their structures were elucidated on the basis of physiochemical properties and spectral data. RESULTS: Ten compounds were isolated and elucidated as uracil (1), 6, 7-dimethoxycoumarin (2), 6, 7, 8-trimethoxycoumarin (3), p-hydroxybenzoic acid ethyl ester (4), 1∶1 mixture of two diastereomers identical with (3RS, 6RS)-2, 6-dimethyl-octa-1, 7-dien-3, 6-diol (5 and 6), kaempferol (7), isofraxidin (8), scopoletin (9), and loliolide (10). CONCLUSION: Compounds 1, 3-6 and 10 are isolated from this plant for the first time and compounds 1, 4-6 are isolated from this genus for the first time.

HPLC Fingerprint Analysis of Caulis of Chimonanthus nitens Combined with Chemometrics Method / 中国药学杂志

OBJECTIVE: To establish the high performance liquid chromatography (HPLC) fingerprint of the caulis of Chimonanthus nitens and evaluate the product quality by chemometrics analysis method. METHODS: The method was developed on an Amethyst C18-H column(4.6 mm×250 mm, 5 μm)by gradient elution with acetonitrile-water (containing 0.1% formic acid) as mobile phase at a flow rate of 0.8 mLmin-1. The column temperature was maintained at 28℃, and the detection wavelength was set at 254 nm. The main characteristic peaks was identified by comparing the retention time and UV absorption characteristics. Then 10 batches of the caulis of Chimonanthus nitens were evaluated by similarity assay, HCA, and PCA. RESULTS: The HPLC fingerprint of the caulis of Chimonanthus nitens was established and three main peaks were identified. The similarity of 10 batches of the caulis of Chimonanthus nitens was about 0.978 0 to 0.991 9. CONCLUSION: The established method can be used for the quality control of the caulis of Chimonanthus nitens.