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Chinese journal of integrative medicine ; (12): 832-839, 2016.
Artigo em Inglês | WPRIM | ID: wpr-301020

RESUMO

<p><b>OBJECTIVE</b>To determine the effect of medicated serum of Chinese herbal compound Naofucong (, NFC) on the microglia BV-2 cells viability and the transcription and expression of interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α) in microglia BV-2 cells to further explore the mechanisms underlying the protective effect of NFC on inflammatory process induced by high glucose.</p><p><b>METHODS</b>The microglia BV-2 cells incubated in vitro were divided into different groups: the control group (25 mmol/L glucose), the model group (75 mmol/L glucose), high glucose media containing different dose medicated serum of NFC. After being cultured for 24 h, changes in IL-6 and TNF-α were measured by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay. The expression of surface marker CD11b of activated microglia was measured by confocal laser scanning microscope and Western blot. Nuclear factor-κB (NF-κB) p-p65 expression was analyzed by Western blot.</p><p><b>RESULTS</b>The model group obviously increased the expression of microglial surface marker CD11b and NF-κB p-p65 (all P<0.01), induced a signifificant up-regulation of release and the mRNA expression of IL-6 and TNF-α (P<0.01 or P<0.05). The medicated serum of NFC could obviously down-regulate the transcription and expression of surface marker CD11 b and NF-κB p-p65 (all P<0.01), and inhibit the mRNA and protein expression (P<0.01 or P<0.05) of inflflammatory cytokines, such as IL-6 and TNF-α, in microglia BV-2 cells cultured with high glucose for 24 h.</p><p><b>CONCLUSIONS</b>The inhibition of microglial activation and IL-6 and TNF-α expression induced by high glucose may at least partly explain NFC therapeutic effects on diabetes-associated cognitive decline diseases. Its underlying mechanism could probably be related to the inhibition of NFC on NF-κB phosphorylation.</p>


Assuntos
Animais , Masculino , Camundongos , Biomarcadores , Metabolismo , Western Blotting , Antígeno CD11b , Genética , Metabolismo , Linhagem Celular , Forma Celular , Sobrevivência Celular , Medicamentos de Ervas Chinesas , Farmacologia , Usos Terapêuticos , Ensaio de Imunoadsorção Enzimática , Imunofluorescência , Glucose , Toxicidade , Inflamação , Tratamento Farmacológico , Patologia , Interleucina-6 , Genética , Metabolismo , Microscopia Confocal , RNA Mensageiro , Genética , Metabolismo , Ratos Wistar , Reação em Cadeia da Polimerase em Tempo Real , Fator de Transcrição RelA , Metabolismo , Fator de Necrose Tumoral alfa , Genética , Metabolismo
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