RESUMO
OBJECTIVE: To prepare and develop a GDH-2 air sampling tube for detecting 12 kinds of chlorobenzenes(CBs) in workplace air and to establish a matching detecting method. METHODS: The self-developed GDH-2 air sampling tube was filled with ion exchange resin and activated carbon, and the mass ratio was 10 ∶1. The GDH-2 air sampling tube was used to collect 12 kinds of CBs with coexistence of gaseous and aerosol in the air. After elution with toluene, they were separated on a chromatographic column and determined by microcell electron capture detector. RESULTS: The quantitative detecting range of the method was 0.51×10~(-3)-6 000.00 mg/L, with the correlation coefficients greater than 0.999 4. The minimum detection concentration was 0.02-61.99 μg/m~3, and the minimum quantitative concentration was 0.05-206.62 μg/m~3. The average desorption efficiency was 90.8%-104.0%. The within-run relative standard deviation(RSD) was 1.0%-5.7%, and the between-run RSD was 3.0%-7.3%. The samples can be stored at room temperature for at least 26 days. CONCLUSION: The self-developed GDH-2 air sampling tube and its matching measuring method can be used for the collection and determination of the 12 kinds of CBs in the air of workplace.
RESUMO
OBJECTIVE: To establish a method for simultaneous determination of 12 kinds of chlorobenzene compound(CBs) in workplace air using portable gas chromatography-mass spectrometry(GC-MS) technique. METHODS: The GDH-3 air sampling tube was developed independently, and were used to collect the 12 kinds of CBs in the vapor state and aerosol state in the air. After elution with toluene solvent, portable GC-MS method was used for detection. Fast chromatographic column was used for separation, and then qualitatively analyzed with retention time and characteristics of the ions, and quantitative analyzed by standard curves. RESULTS: The quantitative determination ranges of the 12 kinds of CBs were 0.20-200.00 mg/L. All the correlative coefficients were greater than 0.998 3. All the minimum quantitative concentration was 0.01 mg/m~3, and all the minimum quantitative mass concentration was 0.04 mg/m~(3 )(15 L sample). The average elution efficiency was 88.97%-116.86%. The within-run and the between-run relative standard deviation was 10.15%-13.48% and 12.87%-19.66%, respectively. All the sampling efficiencies were>90.00%. CONCLUSION: The portable GC-MS technique could be used for rapid qualitative and quantitative detection of 12 kinds of CBs in workplace air.
RESUMO
OBJECTIVE: To establish a method for simultaneous determination of chlorobenzene, p-dichlorobenzene and o-dichlorobenzene in workplace air by portable gas chromatography-mass spectrometry(GC-MS). METHODS: The portable GC-MS heat tracing sampling probe was used for sampling. Samples were separated with LTM DB-5 MS rapid chromatographic column. The qualitative analysis was based on retention times and characteristic ions, and the quantification was based on standard curves. RESULTS: The linear correlation coefficient of this method was higher than 0.999 6. The minimum detectable concentrations were 0.03, 0.03 and 0.06 mg/m~3, and the minimum quantification concentrations were 0.10, 0.10 and 0.20 mg/m~3. The recovery rates were 84.68%-92.61%, 86.27%-93.92% and 82.31%-92.36% respectively for three chlorobenzenes compounds. The within-run relative standard deviations(RSD) were 8.51%-9.34%, 7.93%-9.19%, 5.47%-7.48% respectively for three chlorobenzenes compounds, the between-run RSD were 7.29%-9.73%, 8.08%-10.04% and 5.19%-5.98% respectively for three chlorobenzenes compounds. CONCLUSION: The portable GC-MS could be used for qualitative and quantitative detection of three chlorobenzenes compounds in workplace air.
RESUMO
OBJECTIVE: To evaluate the inhibitory effects and mechanism of Hydrochloric acid chlorobenzene sulperzon (HACS) on neuronal inflammation were studied in order to evaluate possible application of it in AD therapy. METHODS: The release of NO (nitric oxide) by astrocyte was detected by Griess methods and the chemotaxis of mouse macrophage was detected by Boyden chemotaxis chamber. The cell viability was detected by MTT assay. The content of IL-6 and RANTES (T cell expressed and presumably secreted)was determined by ELISA. The expression of mRNA of IL6 and RANTES was detected by RT-PCR. The intracelluar Ca2+ was detected by confocal microscope. RESULTS: HACS efficiently decreased the release of NO from astrocyte stimulated by LPS (1 μg·mL-1), chemotaxis of mouse macrophage stimulated by PAF (5×10-8 mol·L-1), expression of IL-6 and regulated upon activation of RANTES in U251 cells induced by IL-1β (50 ng·mL-1). In addition, HACS significantly inhibited the increase of intracellular Ca2+ in U251 cells induced by Aβ1-42(50 μg·mL-1)/sodium glutamate(100 μmol·L-1)or IL-1β(50 ng·mL-1). CONCLUSION: HACS efficiently inhibites the activation of astrocyte by regulation of intracelluar Ca2+inhibition of chemotaxis and decrease of inflammatory cytokines. Especially, the inhibition of RANTES and intracelluar Ca2+ induced by inflammatory mediators by HACS is firstly reported in this study.
RESUMO
OBJECTIVE:To establish a method for the simultaneous contents determination of chloramphenicol and diphenhydr-amine hydrochloride in Chlorobenzene alcohol solution. METHODS:HPLC was performed on the column of Diamonsil C with mo-bile phase of phosphate buffer-acetonitrile(66.5∶33.5,V/V)containing 0.01 mol/L sodium heptane,column temperature was 35 ℃, flow rate was 1 ml/min,detection wavelength was 258 nm for diphenhydramine hydrochloride and 277 nm for chloramphenicol, the injection volume was 10μl. RESULTS:The linear range was 34.8-139.4μg/ml for chloramphenicol(r=0.999 8)and 93.0-930.5μg/ml for diphenhydramine hydrochloride (r=0.999 8);RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 97.70%-104.02%(RSD=2.2%,n=9)and 96.72%-101.72%(RSD=1.6%,n=9). CONCLUSIONS:The method is specific,accurate with good precision,and suitable for the quality control of Chlorobenzene alcohol solution.
RESUMO
Objective To establish an animal model for immunological contact urticaria in mice.Methods A total of 60 BALB/c mice were randomly and equally divided into 5 groups:anti-dinitrophenol IgE monoclonal antibody (anti-DNP IgE) + 2,4-dinitrofluorobenzene (DNFB) group and anti-DNP IgE + trimellitic anhydride (TMA) group both injected with anti-DNP IgE via tail veins firstly,followed by topical treatment with DNFB and TMA respectively on the ears at 24 hours after the injection,DNFB group,TMA group and normal saline (NS) group all injected with NS via the tail vein firstly,followed by topical treatment with DNFB,TMA and NS on the ears 24 hours after the injection.In the following 14 days,mice were observed daily for the appearance of wheals and for scratching behavior.All the mice were sacrificed at the end of the study followed by determination of the percentage of degranulated mast cells and spleen index as well as observation of pathological changes.Results Wheals were observed in all the mice (12/12) in the anti-DNP IgE + DNFB group,some mice (8/12) in the anti-DNP IgE + TMA group,but not observed in any mice in the other 3 groups.Compared with the NS group,both the anti-DNP IgE + DNFB group and anti-DNP IgE + TMA group showed a significant increase in the percentage of degranulated mast cells (70.21％ ± 26.01％ and 54.25％ ± 39.57％ vs.14.45％ ±6.79％,F=14.41,P=0.000),spleen index (7.54 ± 1.56 and 7.87 ± 1.18 vs.5.37 ± 1.16,F=4.29,P=0.004) and scratching frequency ((31.58 ± 3.58)/h and (22.17 ± 3.81)/h vs.(2.00 ± 0.85)/h at 30 minutes,F =437.86,P ＜ 0.01).Conclusion A stable mouse model for immunological contact urticaria can be established quickly by sensitization with anti-DNP IgE and challenge with DNFB.
RESUMO
0.99. The recovery rates were 72.25% -109.0% with the relative standard deviation (RSD) of 1.82% -11.61% . Conclusion The method is fast,simple,sensitive and needs no organic solvent and it is applicable to the determination of organochlorine pesticides and chlorobenzene compounds in water.
RESUMO
In this study,we isolated and characterized chlorobenzene degrading bacteria from the effluent and sludge samples of one chemical plant.Minimal medium supplemented with chlorobenzene as sole car-bon source was used during the enrichment and domestication process.Seven major bacterial isolates were obtained and purified.Their 16S rRNA genes were amplified by PCR for sequencing and their identities were determined with homology comparisons.Five of the seven isolates belong to Actinomycetales in-cluding Kocuria KD139,Rhodococcus KD140,Rhodococcus KD142,Arthrobacter KD230,and Ar-throbacter KD232;one is classified as Bacillus d KD178;and another one as Stenotrophomonas KD237.The phylogenetic tree was also constructed based on the analysis of 16S rRNA gene sequences.Chloro-benzene concentrations were quantified with gas chromatography to investigate the bio-degradation rates of the isolated strains.Stenotrophomonas KD237 degraded 60.78% chlorobenzene in the minimal medium within 24 h.