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Objective To optimize the extraction conditions for total flavonoids from Cibotium barometz by response surface meth-od(RSM). Methods According to the center combination of Box-Benhnken,using the RSM,the effects of ethanol concentration,the ratio of solid to liquid,the extraction time,and the extraction frequency were studied by central composite design. Results The opti-mal conditions of extraction were as follows:60％ethanol,the ratio of solid to liquid 1:40,refluxing and extracting twice,and 1.5 h for each time. Conclusion The actual extraction yield was 1.44％. The method of extraction has higher extraction efficiency than other methods and can provide a basis for the industrial production of the total flavonoids from S. barometz.
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Objective: To investigate the chemical constituents from the rhizomes of Cibotium barometz. Methods: Macroporous adsorption resin, column chromatography, and preparative HPLC were carried out to isolate and purify the chemical compounds in the 50% ethanol extracts from the rhizomes of C. barometz, and their structures were elucidated by spectroscopic and chemical analyses. The activities of compounds 1 and 2 were tested on in vitro assays of anti-inflammatory, anti-tumor, neuro-protection, anti-diabetes, estrogen receptor agonism, and estrogen receptor antagonism. Results: Nine compounds were isolated and identified as protocatechuic acid-4-O-(6'-O-protocatechuoyl)-β-D-pyranoglucoside (1), resveratrol (2), p-hydroxycinnamic acid (3), p-hydroxycinnamic aldehyde (4), C-veratroylglycol (5), 4-methyl-1,2-dihydroxybenzene (6), protocatechualdehyde (7), p-hydroxybenzoic acid (8), and caffeic acid (9). Compound 1 showed anti-inflammatory activity at the concentration of 10, 1, and 0.1 μmol/L with inhibition rates of 75.3%, 72.0%, and 58.0% to NO release of BAW cells, respectively, and exhibited promising hepatoprotective activity against APAP-induced acute liver damage of HepG 2 cells in vitro as effective as bicyclol. Compound 2 showed anti-inflammatory activity at the concentration of 1 μmol/L with inhabition rate of 50.0%. There were no activity on other assays, such as anti-tumor, estrogen receptor agonism, and estrogen receptor antagonism. Conclusion: Compound 1 is a new compound named biprotocatechuicoside with promising anti-inflammatory and hepatoprotective activity, and compounds 2-5 are isolated from the plants of Cibotium Kaulf. for the first time.
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OBJECTIVE:To compare the differences in the contents of water-soluble total protein,total phenolic acid and total polysaccharides among the water decoctions of crude Cibotium barometz and processed products and to illuminate the effect of pro-cessing on 3 kinds of components of C. barometz. METHODS:UV-visible spectrophotometry was adopted to determine the con-tents of water-soluble total protein,total phenolic acid and total polysaccharides in the water decoction of crude C. barometz and 4 processed products,namely sand-scorch C. barometz,yellow wine C. barometz,salt C. barometz and steamed C. barometz,at wavelengths of 590,760 and 489 nm respectively. RESULTS:The contents of water-soluble total protein in 5 samples were 4.03%,3.32%,3.13%,3.33% and 3.49%,those of total phenolic acid therein were 0.25%,1.34%,1.38%,2.34% and 1.41%,and those of total polysaccharides therein were 28.56%,36.06%,45.21%,49.60% and 49.01%,respectively. CONCLU-SIONS:All above processing methods have an effect to some degree on the contents of the 3 kinds of components of C. barometz, where the contents of water-soluble total protein are lower after processing,while those of total phenolic acid and total polysaccha-rides are higher thereafter.
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Objective: To study the chemical constituents from Cibotium barometz continuously. Methods: Column chromatography and HPLC were carried out to isolate and purify the chemical compounds in the ethanol extract from C. barometz, and their structures were elucidated on the basis of spectroscopy. Results: Six compounds isolated from C. barometz were identified as 6-O-protocatechuoyl-D-glucopyranose (1), 3-O-caffeoyl-D-glucopyranose (2), 1-O-caffeoyl-β-D-glucopyranose (3), caffeic acid (4), protocatechuic acid (5), and p-hydroxybenzoic acid (6). Conclusion: Compounds 1-3 and 6 are isolated from C. barometz for the first time, and compound 1 is a novel penolic glucoside named cibotiumbaroside D.
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Objective: To research on the sublimation and volatile components of Cibotium barometz and to explore its irritant ingredient sources during the processing. Methods: Using the simulation of sand iron, sublimation, steam distillation, and volatile oil extractor to collect the sublimation and volatile components from C. barometz, and using UPLC/Q-TOF-MS for qualitative analysis. Results: A total of 34 compounds were checked out by the four methods and 26 out of them were recommended molecular formula and named, mainly including organic acids, organic acid esters, alkaloids, purines, pyrimidines, small molecular aldehyde ketones, polyphenols, steroids, single terpenes, etc. The molecular volume of the compounds is concentrated within 100-400 ranges. Conclusion: Citramalic acid, 6-gingerol, and 5-methylfurfural have the irritation, which should be the produced irritant components during C. barometz processing.
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Objective: It was to investigate the content alteration of the tannins in cibot rhizome and its processed samples. Method: The casein method was used to determine the content of tannins. Results: The content of tannins decreased after the medicinal material was processed. Conclusion: Processing may decrease the content of tannins in cibot rhizome. The raw was better than the other processed samples if the tannins were used as active constituents.