Summary and analysis on external quality assessment results of clinical immunology during 2009 - 2013 / 国际检验医学杂志

Objective To perform the summary and analysis on the external quality assessment(EQA) results of the clinical im‐munology during 2009 - 2013 to find out the possible problems existing in the laboratory work of the clinical immunology for con‐tinuously improve the quality of clinical immunological detections and ensuring the accuracy of clinical immunological test results . Methods The results of the external quality assessment in the clinical Immunology participating in the National Center for Clinical and Laboratory and the Guangxi Center for Clinical Laboratory during 2009 - 2013 were performed the statistical analysis and sum‐mary .Results 600 samples were detected during these 5 years .The performance was worst in 2012 ,the annual coincidence rate of the results was 98% ;and which in other 4 years was 100% ;each 1 sample of result in the two items of anti‐HCV and anti‐TP was unconformity during these 5 years ,the total coincidence rate was 98 .6% ;the total coincidence rates of HBsAg ,HBsAb ,HBeAg , HBeAb ,HBcAb and anti‐HIV were 100% .Conclusion By participating in the external quality assessment of the clinical immunolo‐gy and retrospectively analyzing the results ,the possible problems would be found and some effective improvement measures could be formulated in time for improving the quality of examinations and ensuring to provide reliable laboratory data for clinic .

Teaching practice of “paper type test report” / 中国现代医生

Objective To assess the teaching effect of "paper type test report" teaching mode in medical laboratory specialty undergraduate class. Methods In the teaching of "Clinical Immunology Examination" and "Clinical Thera-peutic Drug Monitoring Technology", the medical laboratory specialty undergraduate class of Grade 2009 were divided into test group(n=28) and control group(n=27)at random,"paper type test report"and traditional teaching modes were applied respectively. The teaching effects were evaluated by experiment examination result, practice result, graduation thesis and class theory achievement, etc. Results Graduation thesis scores of experimental group were significantly bet-ter than that of the control group (P<0.05). In the teaching of"the Clinical Therapeutic Drug Monitoring Technology", the experimental results, the experimental results, the theory exam overall rating scores were better than control group, and the differences were statistically significant (P<0.05). Conclusion Teaching model of "paper type test re-port"plays a positive role in improving of students' writing ability and consolidating of theory knowledge ,etc.

Practice of case-based PBL teaching model in clinical immunology and laboratory teaching / 中华医学教育探索杂志

Objective To train medical students' competence of independent thinking and problem solving and to improve the quality of teaching.MethodsThe case-based PBL teaching model was introduced to teaching practice for 25 undergraduates majoring in medical laboratory.The teaching process included pre-class mobilization,question,self-study,discussion and summary.Effectiveness of teaching was evaluated with questionnaire.Results For these medical students,the enthusiasm of learning ( 83.3％ ),ability of self-learning and information seeking ( 79.2％ ),ability of discussing and problem solving ( 79.2％ ),ability of scientific thinking and clinical reasoning ( 79.2％ ) were significantly improved.Teaching satisfaction rate of case-based PBL teaching model was up to 95.8％.ConclusionBetter results of teaching were achieved and the case-based PBL teaching model is worth spreading.

Identification of Major and Minor Allergens of Black Tiger Prawn (Penaeus monodon) and King Prawn (Penaeus latisulcatus)

Background: Prawns and shrimp are a frequent cause of seafood allergy mediated by IgE antibodies. Penaeus monodon and Penaeus latisulcatus, commonly known as black tiger prawn and king prawn, respectively, are among the most frequently consumed prawns in Malaysia. The aim of thi s study was to identify the IgE-binding proteins of these 2 prawn species. Methods: Raw and boiled prawn extracts were prepared and then resolved by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). IgE-immunoblotting was then performed using sera from patients with positive skin prick tests to the raw prawn extracts. Results: SDS-PAGE analysis of the raw extracts of both prawn species revealed 23 protein bands; the boiled extracts yielded fewer protein bands. The bands in the range of 40 to 100 kDa were sensitive to heat and therefore were not found in the boiled extracts. Immunoblot of raw extracts of black tiger prawns and king prawns yielded 14 and 11 IgE-binding proteins, respectively, with molecular weights of between 15 and 200 kDa. Proteins at 36, 42, and 49 kDa were detected as the major allergens in both species of prawns. A protein of 75 kDa was also identified as a major allergen in black tiger prawns. Other potential allergens were also observed at various molecular masses. Conclusion: Proteins of 36, 42, and 49 kDa were identified as the major allergens of both species of prawns. The 36 and 42 kDa proteins are hypothesised to be tropomyosin and arginine kinase, respectively. A high molecular weight protein of 75 kDa was found to be an additional major allergen in black tiger prawns.

Nosocomial Infection in Clinical Immunology Laboratory:Risk Factors and Control Strategies / 中华医院感染学杂志

OBJECTIVE To explore the risk factors and control strategies of nosocomial infection in clinical immunology laboratory.METHODS The positive detection rates of HBV marker and antibodies to HAV,HCV,HEV,HIV,TP,and TB in total of 91 877 samples in clinical immunology laboratory during Jul 2004 and Jul 2005 were retrospectively surveyed and statistically analyzed.RESULTS The number of positive specimens of HBV marker and antibodies to HAV,HCV,HEV,HIV,TP,and TB were respectively 8 376,7,26,24,107,3,522,and 52 and the positive detection rates of these items were respectively 16.68%,0.43%,0.20%,2.22%,6.49%,0.03%,4.75%,and 4.61%.CONCLUSIONS It is very important to understand the infection sources and risk factors in clinical immunology laboratory in order to strengthen management of hospital infection and protection of occupational exposure.

The Experiences of Teaching Clinical Immunology / 中华医学教育探索杂志

Clinical immunology should display the advanced and applied characteristics of immunology,and show the speciality of clinical practice.This course is a bridge between fundamental immunology and clinical teaching so that the medicos should get better foundation for the future learning.

Construction and application of clinical immunology and inspection of net information / 中华医学教育探索杂志

Objective To connect the content of clinical immunology and inspection with network,so that it is more convenient for medical students to understand and study this course,and the learners’interest in learning is aroused.Methods FrontPage 2003 web page authoring software,as well as animation software(flash) and picture editing software(Photoshop) were used to make a vivid image of the teaching content be shown through the web page,and effectiveness evaluation was done through peer review and students survey about the software.Results Using FrontPage to create web pages and change knowledge into learning pattern.got good results in studetns’self-study,and the"online self-test"section was added,which would more effectively check study effect.There were good student feedbacks.Conclusion Applying this learning pattern to study will play a very good role in deepening students’understanding of curriculum,improving their study efficiency and stimulating their interest in learning.

Adverse drug reactions of glucocorticoid to asthmatic patients at the department of allergology and clinical immunology in Bach Mai Hospital (1998-2002)

GC is wonderful drug to prevent and control bronchial asthma. Methods: 1026 inpatients with asthma presented at the Department of Allergy and Clinical Immunology were included in this study. Cross - sectional surveys and retrospective studies. Objectives: Study on situation of using GC of asthmatic patients. Describe adverse drug reactions of glucocorticoid to asthmatic in - patients at the Department of Allergy and Clinical Immunology. Results: All of 1026 patients used Gc. Prednisolon, solu - medrol, depersolon and pulmicort were most used medications. There were 295 asthmatic patients have side effects (28.75%) with 24 types of symptom, 15 diseases and syndromes are resulted: Cushing syndrome, gastric ulcer, diabetes, hypertension, ponosis, retarded development... Almost systemic organs of the patients were influenced negatively by Gc. It has relationship among the way to use, the time in used GC and adverse drug reactions of GC. Conclusions: GC caused many adverse drug reactions to asthmatic patients.

Study on allergic reactions due to specific for gout's treatment at the department of allergology and clinical immunology of Bach Mai hospital

Author studied 21 allergic patients due to specific for Gout'streatment from 1995 to 2001 at the Department of Allergology and Clinical Immunology of Bach Mai Hospital with method "cross- sectional surveys". Results showed that: - Allopurinol, colchicin and both go together are specific for Gout'streatment and It caused of. allergic reactions of 21 patients. - Main symptoms of allergic patients of specific for Gout'streatment were erythema, itching and fever The main clinical form is Erythrodermie; Allergic reations were mostly nappened late (after 24 hours); Elevated ESR, white blood cell, uremia, SGOT SGPT and proteinuria are main changes of tests. -It takes a long time to treat allergic patients (13,54 :t 10,17 days); Solution of methyl-prednisolon and dimedrol are common medications to treat this patients; No allergic patient is died due to the specific for Gout'streatment at The Departma

Application of test-driven teaching in clinical immunology course / 中华医学教育探索杂志

The application of test-driven methods in clinical immunology course can arouse students'interest,and give full play of their go-aheadism and creativity.Test-driven teaching methods surpasses the traditional teaching methods.

Exploration and practice on the model curricula development of clinical immunology examination / 中华医学教育探索杂志

The construction of the model curricula is the important branch of teaching quality engineering. The key to the development of the model curricula lies in the reform of the teaching model. As required to the national standards of the model curricula and to the purpose of innovative educational philosophy,with the years of continuous exploration and practice,the clinical immunology examination curriculum has kept developing from the teaching staff,teaching condition,content of course,teaching method and means etc.,which has brought about satisfactory teaching effects. Thus it is forming the model curricula with special features.

Quantitative analysis for individual baseline drift for immunoglobulin and complement within 24 h / 中国免疫学杂志

Objective:To analyze quantitatively individual baseline drift of immunoglobulin and complement within 24 h.Methods:Blood samples were drawn from adult volunteers on day 1 and day 2 at 7.00 am.Immunoglobulin and complement including IgG,IgA,IgM and C3,C4 were measured with automatic immunobiochemistry analyzer.The rate of changes between two times of measured value,baseline drift,could be calculated.Results:The individual baseline drifts of measured values were from 6.12% to 7.97% (mean=7.22%) in 5 immunological parameters.There were significations between two groups within 5 immunological parameters (P