Neuroprotective mechanisms of cocaine-and amphetamine-regulated transcript peptides in brain ischemia / 国际脑血管病杂志

Cocaine-and amphetamine-regulated transcript(CART) peptides are endogenous neurotransmitters with important roles in a number of physiological and pathological processes in vivo.Many studies suggested that CART is widely distributed in the central nervous system,and it has some central protective effects.This article reviews the recent progress in research on the protective effect of CART on cerebral ischemia and its mechanisms.

Effect of cocaine-amphetamine-regulated transcript peptide on the content of 4-hydroxy-2-noneral and infarct volume after cerebral ischenia/reperfusion in mice / 国际脑血管病杂志

Objective To investigate the effect of cocaine-amphetamine-regulated transcript peptide (CART) on the content of 4-hydroxy-2-noneral (HNE) and infarct volume after cerebral ischemia/reperfusion in mice.Methods A total of 96 healthy male mice were randomly divided into four groups:ischemia/reperfusion (n =27),CART (n =27),normal saline control (n =27) and sham operation (n =15) groups.A middle cerebral artery occlusion (MCAO) model was induced.Two hours after MCAO,CART 55-102 and equivalent normal saline were injected respectively via the tail veins of mice in the CART group and the normal saline control group,and then they were injected every other 24 hour.The neurological scores,infarct volume and the HNE content of lipid metabolism of oxidative stress were performed and detected respectively at 12,24,48 and 72hours after reperfusion.Results CART could significantly improve the neurological deficit scores (all P ＜0.05) and reduce infarct volume (all P＜0.05) at different time points after ischemia/reperfusion.The content of HNE was upregulated (all P＜0.05) at different points after referfusion.CART could significantly down-regulate the increased HNE levd in brain after ischemia (all P＜0.05).Conclusions CART may protect ischemic brain injury in mice by inhibiting lipid peroxidation.

Effect of CART55-102 protein vaccine on morphine analgesia and tolerance / 第二军医大学学报

Objective: To study the effect of cocaine- and amphetamine-regulated transcript (CART) protein vaccine on morphine analgesia and tolerance. Methods: The expression plasmid pGEX-4T3-CART was constructed by gene cloning. The CART protein was purified by glutathione s-transferase (GST)-affinity chromatography. The experiment included 6 groups: blank control, normal saline (NS) GST + Freund's adjuvant, and CART protein vaccine (5 μg, 10 μg and 20 μg) groups. After immunization for twice, all groups were tested in hot plate. Morphine analgesia effect was evaluated through s. c. injection with 6 mg/kg morphine solution, calculated by MPE%. Then morphine tolerance model was established, and the tolerance to morphine was tested by s. c. injection with 6 mg/kg morphine 12 h after the last injection. Results: CART vaccine itself had no pronounced effect on the pain threshold (P>0.05). CART vaccine at 10 μg significantly depressed the analgesic effect of morphine analgesia (P<0.05). Compared with NS group, vaccine groups showed a potential antagonizing tolerance effect, especially in the 10 μg group, with the MPE% significantly increased (P<0. 05). Conclusion: CART vaccine itself has no influence on the pain response; however, it can impair the analgesia effect of morphine and can antagonize the analgesia tolerance to morphine.

Effect of CART55-102 protein vaccine on morphine analgesia and tolerance / 第二军医大学学报

0.05).CART vaccine at 10?g significantly depressed the analgesic effect of morphine analgesia (P