Osmoregulatory Appraisal of Some Osmoprotectants on Hydrolytic Activities of Some Enzymes on Seeds of Two Water Stressed Cultivars of Sorghum bicolor (Moench)

Enzymes play significant roles in metabolic processes of seeds. Therefore, this study evaluated osmoregulatory potential of some osmoprotectants on activities of some hydrolytic enzymes in the seeds of two cultivars (SOSAT.C-88 and CV. LCIC 9702) of sorghum bicolor. Matured seeds of the two cultivars were harvested and prepared for alpha, beta, total amylase and proteinase activities assay. The osmoprotectants produced significant variations on the enzymes at 10 and 14 days (DA) of 8 weeks after treatments (WAT). Seeds of well-watered SOSAT.C-88 produced higher alpha (2.10 IU/ml), beta (1.70 IU/ml) and total amylase activities (3.30 IU/ml) at 14 days (DA). Higher alpha (2.01 IU/ml and total amylase activities (2.61 IU/ml) were recorded in the seeds of CV. LCIC 9702 well-watered at 14 days DA 8WAT. Furthermore, total amylase activities (3.87 IU/ml) were recorded in the seeds produced by CV. LCIC 9702 well-watered at 14 days DA. Significant increase was noticed in beta (1.14 IU/ml) and alpha amylase (1.58 IU/ml) in the seeds of CV. LCIC 9702 treated with mycorrhiza. CV. LCIC 9702 well watered produced highest proteinase activities (1.57 U/ml) while least of the parameters were recorded in SOSAT.C-88 and CV. LCIC 9702 droughted. In conclusion, the osmoprotectants had regulatory effects on the activities of hydrolytic enzymes therefore the use of the osmoprotectants in farming should be encouraged.

Effectiveness of mussels (Mytella falcata) in malnourished children's recovery living in the slums in Maceió, Alagoas / Eficácia do sururu (Mytella falcata) na recuperação de crianças desnutridas, moradoras de favelas de Maceió, Alagoas

Abstract Objectives: to assess the efficacy of mussels (Mytella falcata) in malnourished children's recovery. Methods: 64 chronically malnourished children were accompanied for 12 months and attended at the Centro Recuperação e Educação Nutricional (Recovery Center and Educational Nutrition). The children were paired by age and malnutrition level forming three groups, which they received a balanced diet for nutritional recovery differing only on protein source. The group was offered (1) preparation of red meat, group (2) preparation of mussels and group (3) preparation of mussels in coconut milk. Results: the anthropometric assessment revealed that the children obtained a mean increase in the Z score in A-I indice of 0.70 for the group who red meat, 0.62 for the group who had mussels and 0.57 the group who had mussels cooked in coconut milk (p<0,05). An observation was made on a reduction in the prevalence of anemia with 22,8% (p=0,002), 27.8% (p=0.,007) and 42.4% (p 0.001) in groups 1, 2 and 3, respectively. Conclusion: the preparation of mussels cooked in or not in coconut milk can be an effective substitution for meat in combating child malnutrition and anemia and may be included in the children's institutions menus and in the programs that aim for children's nutritional recovery.

Resumo Objetivos: avaliar a eficácia do sururu (Mytella falcata) na recuperação de crianças desnutridas. Métodos: foram acompanhadas por 12 meses 64 crianças desnutridas crônicas atendidas no Centro Recuperação e Educação Nutricional. As crianças foram pareadas por idade e grau de desnutrição formando 3 grupos, os quais receberam dieta balanceada para a recuperação nutricional diferindo apenas na fonte de proteína. Foi ofertada ao grupo (1) preparação de carne bovina, ao grupo (2) preparação de sururu e ao grupo (3) uma preparação de sururu com leite de coco. Resultados: a avaliação antropométrica revelou que as crianças obtiveram um incremento médio em escore Z no índice A-I de 0,70 para o grupo da carne, 0,62 no grupo que recebeu sururu e 0,57 no grupo que recebeu sururu com leite de coco (pD0,05). Constatou-se ainda redução da prevalência da anemia nos grupos com percentual de redução de 22,8% (p=0,002), 27,8% (p=0,007) e 42,4% (pD0,001) nos grupos 1, 2 e 3, respectivamente. Conclusões: a preparação sururu com ou sem leite de coco pode ser um substituto eficaz da carne bovina no combate a desnutrição infantil e da anemia, podendo ser incluída no cardápio de instituições infantis e em programas que visem à recuperação nutricional de crianças.

6 weeks consumption of pure fresh coconut milk caused up-regulation of eNOS and CSE protein expression in middle-aged male rats

Coconut milk (CCM) has been an important cooking ingredient in the Asia-Pacific region since ancient time. Due to its high content of saturated fatty acids, it has been considered atherogenic. We have tested if chronic consumption of fresh coconut milk by middle-aged male rat affects vascular function, plasma glucose and lipid profiles. Compared to control, CCM caused lower maximal contraction to phenylephrine of thoracic aortic rings and increased relaxation to acetylcholine that was abolished by N G-nitro-L-arginine (L-NA) or disruption of the endothelium. DL-propargylglycine caused slight increase in baseline tension of L-NA treated aortic rings of CCM-treated rats and produced higher contractile response of the aortic rings to low concentrations of phenylephrine. The aortic eNOS- and cystathionine-γ-lyase(CSE) proteins expression of the CCM-treated rats were also higher than in controls. Except for lower fasting plasma glucose there were no changes in blood chemistry for the CCM treated rats. CCM consumption caused up-regulation of eNOS and CSE protein expression which resulted in increased production of NO and H2S from the blood vessels with attenuation of vasocontraction to phenylephrine and increased relaxation to acetylcholine. These novel benefits may be expected to reduce the development of cardiovascular risk factors in the aging rat

Effect of picloram, additives and plant growth regulators on somatic embryogenesis of Phyla nodiflora (L.) Greene

The present study describes the plant regeneration via somatic embryogenesis in suspension culture derived from the leaf and stem explants of Phyla nodiflora. The medium type, plant growth regulators, complex extract (coconut milk and malt extract) and anti-oxidant (activated charcoal, ascorbic acid, Polyvinylpyrrolidone and citric acid) markedly influenced the embryo regeneration of P. nodiflora. MS with 2,4-D and activated charcoal (10 mg/L) gave the highest stimulation of embryogenic callus growth. Optimized callus was transfered into suspension culture, which showed the globular, heart shaped embryos in MS with 2,4-D + BA + picloram (0.1 mg/L), coconut milk (10 ml/L), citric acid (100 mg/L) on 6th subcultures. Further development stages such as torpedo and cotyledonary stage embryos and fostered maturation of embryos were observed at 8th and 10th subculture. However, the high frequency embryo germination and plantlet (45 plants/20 mg cotyledonary stages embryos) formation was obtained in half-strength MS medium without growth regulators from cotyledonary embryos. All the plantlets established in the field exhibited morphological characters similar to those of the mother plant.